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Molecules 2014, 19(4), 4779-4790; doi:10.3390/molecules19044779

Optimization of Fermentation Conditions for the Production of the M23 Protease Pseudoalterin by Deep-Sea Pseudoalteromonas sp. CF6-2 with Artery Powder as an Inducer

1
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
2
Marine Biotechnology Research Center, Shandong University, Jinan 250100, China
3
Collaborative Innovation Center of Deep Sea Biology, Shandong University, Jinan 250100, China
*
Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 12 April 2014 / Accepted: 14 April 2014 / Published: 16 April 2014
(This article belongs to the Section Natural Products)
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Abstract

Proteases in the M23 family have specific activities toward elastin and bacterial peptidoglycan. The peptidoglycan-degrading property makes these proteases have potential as novel antimicrobials. Because M23 proteases cannot be maturely expressed in Escherichia coli, it is significant to improve the production of these enzymes in their wild strains. Pseudoalterin is a new M23 protease secreted by the deep-sea bacterium Pseudoalteromonas sp. CF6-2. In this study, the fermentation conditions of strain CF6-2 for pseudoalterin production were optimized using single factor experiments and response surface methodology to improve the enzyme yield. To reduce the fermentation cost, bovine artery powder instead of elastin was determined as a cheap and efficient inducer. Based on single factor experiments, artery powder content, culture temperature and culture time were determined as the main factors influencing pseudoalterin production and were further optimized by the central composite design. The optimal values of these factors were determined as: artery powder of 1.2%, culture temperature of 20.17 °C and culture time of 28.04 h. Under the optimized conditions, pseudoalterin production reached 100.02 ± 9.0 U/mL, more than twice of that before optimization. These results lay a good foundation for developing the biotechnological potential of pseudoalterin.
Keywords: pseudoalterin; fermentation; response surfaces methodology; M23 proteases; deep-sea bacterium pseudoalterin; fermentation; response surfaces methodology; M23 proteases; deep-sea bacterium
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhao, H.-L.; Yang, J.; Chen, X.-L.; Su, H.-N.; Zhang, X.-Y.; Huang, F.; Zhou, B.-C.; Xie, B.-B. Optimization of Fermentation Conditions for the Production of the M23 Protease Pseudoalterin by Deep-Sea Pseudoalteromonas sp. CF6-2 with Artery Powder as an Inducer. Molecules 2014, 19, 4779-4790.

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