Molecules 2014, 19(3), 3149-3159; doi:10.3390/molecules19033149
Article

5-Azacytidine Induces Anoikis, Inhibits Mammosphere Formation and Reduces Metalloproteinase 9 Activity in MCF-7 Human Breast Cancer Cells

1,2,†email, 3,†email, 3email, 3email, 4email, 5email, 6,7,* email and 1,3,8,* email
Received: 24 January 2014; in revised form: 7 March 2014 / Accepted: 7 March 2014 / Published: 13 March 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Cancer stem cells are a subset of cancer cells that initiate the growth of tumors. Low levels of cancer stem cells also exist in established cancer cell lines, and can be enriched in serum-free tumorsphere cultures. Since cancer stem cells have been reported to be resilient to common chemotherapeutic drugs in comparison to regular cancer cells, screening for compounds selectively targeting cancer stem cells may provide an effective therapeutic strategy. We found that 5-azacytidine (5-AzaC) selectively induced anoikis of MCF-7 in suspension cultures with an EC50 of 8.014 µM, and effectively inhibited tumorsphere formation, as well as the migration and matrix metalloproteinases-9 (MMP-9) activity of MCF-7 cells. Furthermore, 5-AzaC and radiation collaboratively inhibited MCF-7 tumorsphere formation at clinically relevant radiation doses. Investigating the underlying mechanism may provide insight into signaling pathways crucial for cancer stem cell survival and pave the way to novel potential therapeutic targets.
Keywords: cancer stem cell; breast cancer; anoikis
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MDPI and ACS Style

Chang, H.-W.; Wang, H.-C.; Chen, C.-Y.; Hung, T.-W.; Hou, M.-F.; Yuan, S.-S.F.; Huang, C.-J.; Tseng, C.-N. 5-Azacytidine Induces Anoikis, Inhibits Mammosphere Formation and Reduces Metalloproteinase 9 Activity in MCF-7 Human Breast Cancer Cells. Molecules 2014, 19, 3149-3159.

AMA Style

Chang H-W, Wang H-C, Chen C-Y, Hung T-W, Hou M-F, Yuan S-SF, Huang C-J, Tseng C-N. 5-Azacytidine Induces Anoikis, Inhibits Mammosphere Formation and Reduces Metalloproteinase 9 Activity in MCF-7 Human Breast Cancer Cells. Molecules. 2014; 19(3):3149-3159.

Chicago/Turabian Style

Chang, Hsueh-Wei; Wang, Hui-Chun; Chen, Chiau-Yi; Hung, Ting-Wei; Hou, Ming-Feng; Yuan, Shyng-Shiou F.; Huang, Chih-Jen; Tseng, Chao-Neng. 2014. "5-Azacytidine Induces Anoikis, Inhibits Mammosphere Formation and Reduces Metalloproteinase 9 Activity in MCF-7 Human Breast Cancer Cells." Molecules 19, no. 3: 3149-3159.


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