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Molecules 2014, 19(2), 2299-2329; doi:10.3390/molecules19022299
Published: 21 February 2014
Abstract: Carnosinases are Xaa-His dipeptidases that play diverse functions throughout all kingdoms of life. Human isoforms of carnosinase (CN1 and CN2) under appropriate conditions catalyze the hydrolysis of the dipeptides carnosine (β-alanyl-l-histidine) and homocarnosine (γ-aminobutyryl-l-histidine). Alterations of serum carnosinase (CN1) activity has been associated with several pathological conditions, such as neurological disorders, chronic diseases and cancer. For this reason the use of carnosinase levels as a biomarker in cerebrospinal fluid (CSF) has been questioned. The hydrolysis of imidazole-related dipeptides in prokaryotes and eukaryotes is also catalyzed by aminoacyl-histidine dipeptidases like PepD (EC 188.8.131.52), PepV (EC 184.108.40.206) and anserinase (EC 220.127.116.11). The review deals with the structure and function of this class of enzymes in physiological and pathological conditions. The main substrates of these enzymes, i.e., carnosine, homocarnosine and anserine (β-alanyl-3-methyl-l-histidine) will also be described.
Carnosinases are Xaa-His dipeptidases which belong to the metallopeptidase family M20 A of the metallopeptidase H clan, that play diverse functions throughout all kingdoms of life, ranging from a general role in the hydrolysis of late products of protein degradation to specific biochemical functions in protein maturation, tissue repair, and cell-cycle control . The existence of an enzyme with the ability to hydrolyze the peptide bond in carnosine (β-alanyl-l-histidine, CAR) was first demonstrated by Hanson and Smith . They named the enzyme carnosinase and classified it as a metal-ion-activated aminopeptidase, the activators being Mn2+ and Zn2+. More than 30 years later, Lenney et al. published the first molecular and functional characterization of human tissue  and serum carnosinase  Only in 2003 the gene sequences coding for the human serum (CNDP1 or CN1) and tissue (CNDP2 or CN2) carnosinase were identified . This paper represents a milestone for the subsequent research about carnosinase. The same authors, a joint team of academic and industrial researchers, previously applied for two patents concerning the potential application of the human carnosinase isoforms, as indicative of the scientific and commercial interest that these enzyme might have.
The dyshomeostasis of carnosinase expression and activity cause several physiological dysfunctions and diseases, such as diabetes, ischemia, neurological diseases, wound healing, ocular diseases, etc. Most of them are due to the related dyshomeostasis of the main carnosinase substrate, carnosine.
Carnosine was the first peptide ever isolated from natural material by Gulewitsch and Amiradzibi . The natural compound has been found to be widely distributed in several animal tissues. The wide range of protective properties (pH-buffering properties, antioxidant agent wound healing promoter, ion-chelating agent, especially for CuII and ZnII) makes carnosine of great interest for scientific and commercial uses, especially in the neuroprotection of oxidative stress-driven disorders .
Several reviews about the physiological role, the biological proprieties and the structural action of carnosine have been published , but none on the important proprieties of the carnosine-degrading enzymes, taking also into account that increased levels of carnosinase have been found in aging , whereas some neurological disorders, such as mild dementia, are characterized by reduced carnosinase levels .
This review deals with the structure and function of this class of enzymes in physiological and pathological conditions. Detailed features of carnosine and other histidine-containing dipeptides will be also described.
2. Carnosinase Substrates
Carnosine (β-alanyl-l-histidine, CAR), anserine (β-alanyl-3-methyl-histidine, ANS) and homocarnosine (γ-aminobutyryl-l-histidine, HCAR) are the three most representative compounds of the histidine dipeptides (Figure 1), widely distributed in mammals in different amounts, depending on the species and the tissue considered [11,12].
CAR is synthesized from β-alanine and l-histidine by an ATP-dependent carnosine synthase enzyme (EC 18.104.22.168) (Figure 2), which has been detected in the extracts of chicken muscle and mouse brain [13,14]. Moreover, CAR synthase activity has been detected in glia cells – particularly in oligodendrocytes – derived from the rat brain  and shown to also catalyze the synthesis of homocarnosine.
Two proteins encoded by different genes were shown to degrade carnosine in humans . The first one (CN1, EC 22.214.171.124), also named “human serum carnosinase”, breaks down both carnosine and homocarnosine and is found in serum and brain tissue with high specificity (Figure 3A). The second one (CN2, formerly named “human tissue carnosinase”, EC 126.96.36.199) is a Mn2+ dependent cytosolic enzyme ubiquitously expressed in human tissues. This enzyme is now named “cytosol nonspecific dipeptidase,” because it does not degrade homocarnosine and exhibits a rather broad specificity toward various dipeptides (Figure 3B). Recently, it was reported that a mammalian gene with unknown function, ATPGD1, encodes an enzyme capable of synthesizing CAR and HCAR that has 15- to 25-fold higher catalytic efficiency with β-alanine than with γ-aminobutyrate .
ANS is synthesized from the enzymatic condensation of β-alanine with Nτ-methylhistidine through carnosine synthase in the brain and lens , and hydrolyzed to histidine by anserinase (Xaa-methyl-His dipeptidase, EC 188.8.131.52) in the brain and eye [18,19]. However, the main synthetic pathway of ANS occurs by N-methylation of carnosine, a reaction catalyzed by carnosine N-methyltransferase .
Carnosine is found in low concentrations (below 100 nM) in blood and the cerebrospinal fluid (CSF). HCAR concentration is also low in blood, while its concentration in the brain is 100-fold larger than that of carnosine . Moreover, as for CAR, the cerebral content of HCAR is age-related, being three to six times larger in adults than in infants . Thus, it has been suggested to be a precursor for the neurotransmitter GABA, hence serving as an important inhibitory neuromodulator in human neocortex .
The tissue content of histidine dipeptides are regulated by the activities and localization of carnosine synthetase and carnosinases. In humans, the diet has been proposed to represent the main source of histidine dipeptides . The β-alanyl containing peptides (e.g., anserine and carnosine) are mainly found in mammalian skeletal muscle, whereas the γ-aminobutyryl-containing peptides (e.g., homocarnosine) are typical of the central nervous system. It remains unclear whether human muscle carnosine concentrations are stable . Whereas it has been described that muscle carnosine concentrations are not affected by age, fitness or clinical status , a change in muscular carnosine concentrations probably caused by physical activity and reduced meat intake has been reported [27,28]. Also oral ingestion of β-alanine, the rate-limiting precursor in carnosine synthesis, increases carnosine synthesis in the muscle .
2.1. Biological Role of Carnosinase Substrates
The biological function of CAR is not fully understood, though it has been shown to possess a number of important properties including proton buffering capacity , antioxidant activity [31,32] and chelating ability [33,34,35]. Several studies have also demonstrated that CAR inhibits metastasis  and non-enzymatic glycosylation of protein , prevents the formation of protein-protein cross links by reacting with protein-carbonyl groups , delays diabetic deterioration , and influences glucose metabolism . In the brain, CAR is primarily found in glial and ependymal cells [41,42], while peptide transporter 2 (PepT2) mediates the dipeptide transport in astrocytes . In recent years, the occurrence of CAR and its analogues homocarnosine and anserine in CNS and their age-related alterations [44,45] suggested a therapeutic potential in neurodegenerative diseases [46,47]. Carnosine has been shown to be neuroprotective because of its capacity to counteract both oxidative  and nitrosative stress [49,50] related to several pathological conditions [51,52], including ischemia [53,54,55]. Recently, it has been demonstrated that CAR not only prevents the up-regulation of iNos and the induction of both HO-1 and Hsp-70 following strong nitrosative conditions , but also possesses NO free-radical scavenging ability and NO-trapping capacity in cell-free experiments [57,58]. Furthermore, CAR has been found to protect cells from amyloid-β induced toxicity  by its ability to inhibit protein aggregation by perturbing the H-bond network in and around the central hydrophobic cluster  and to avoid the formation of glycation end-products . Interestingly, carnosine plasma levels have been found to be lower in patients with Alzheimer’s disease (AD) than in age-matched controls . Exogenously administered CAR can cross the blood-brain barrier , but its efficacy as drug is a challenge due to the presence of carnosinase enzymes acting as endogenous dipeptidases .
Carnosine acts as a natural inhibitor of the angiotensin converting enzyme . It restores erythrocyte deformability , and reduces the synthesis of matrix proteins such as fibronectin and collagen type VI of podocytes and mesangial cells . Although the function of carnosine is better described, many studies showed that also anserine seems to have several protective functions. Similar to carnosine, anserine was showed to affect renal sympathetic nerve activity , reduces blood glucose , increases the contribution of the non-bicarbonate buffering action and decreases the bicarbonate buffering action in blood , acts as effective trans-glycating agents in decomposition of aldose-derived Schiff bases , protects neuronal cells against reactive oxygen species , shows dose-dependent angiotensin converting enzyme inhibitory activity , acts as peroxyl radical scavenger to protect the protein modification  and reacts as quencher of cytotoxic carbonyls . Beside these similarities, there is some evidence that both dipeptides have also different functions. Whereas carnosine facilitates NO production in endothelial cells, anserine failed to increase NO production . Treatment with carnosine, but not anserine, was able to significantly reduce infarct volume and improve neurological functions . Further, an increase in the antitumor activity of doxorubicin  was only described for anserine. Beside these functions, the role of anserine in the kidney is not yet known and remains to be addressed.
Carnosine can reduce telomere shortening rate possibly by protecting telomeres from damage. Cells continuously grown in 20 mM carnosine exhibited a slower telomere shortening rate. The authors suggest that the reduction in telomere shortening rate and damages in telomeric DNA made an important contribution to the life-extension effect of carnosine .
Appreciable levels of l-carnosine have been found in transparent human lenses which are markedly depleted in mature cataracts . The concentration of carnosine in transparent crystalline lenses detected was about 25 µM. At different stages of cataract development, the level of carnosine fell, reaching about 5 µM. Research with N-acetylcarnosine (NAC), an ophthalmic prodrug of l-carnosine, demonstrates that it is effective not only in preventing cataracts but also in treating them. NAC has been shown to improve vision by partially reversing the development of the cataract, thus increasing the transmissivity of the lens to light .
The clinical evidence has also been reported that carnosine, that finds its way into the aqueous humor and the crystalline lens through the topically applied NAC admixed with carboxymethylcellulose, is able to reduce telomeric attrition in the lens epithelial cells. This is due to a diminution in the oxidative stress, thus preventing the expression of the senescent phenotype of the lens epithelial cells .
Carnosine and its related-dipeptides are also efficient metal chelating agents (mainly for copper(II) and zinc(II)). Their copper complexes exhibit superoxide dismutase (SOD)-like activity , whereas ZnII-carnosine complex (called Polaprezinc) is effective for the repair of ulcers and other lesions in the alimentary tract [81,82]. Noteworthy, carnosine complexed to zinc(II) is able to induce the expression of important anti-oxidative stress proteins and enzymes, such as heme oxygenase 1 , Hsp72 , Hsp70 . Although such studies have not been carried out in cerebral tissues, the involvement of these stress proteins in any type of both physiological and pathological oxidative stress-induced pathways , further emphasizes the crucial role of carnosine and its metal complexes in living systems.
Very recently, it has also been proven for the first time that carnosine is able to chelate the intracellular zinc(II) , whose mobilization was ROS-mediated. The scavenger activity of carnosine against ROS might represent a further protective effect on the reduction of ROS-mediated zinc(II) release.
For these reasons, it may act as neuroprotective agents in copper-dependent toxic conditions and as antioxidants in physiological and pathological conditions [7,88]. Indeed, the copper- and zinc- mediated neurotoxicity involved in several pathologies, such as amyotrophic lateral sclerosis, Alzheimer’s, Menkes’s, Parkinson’s, Pick’s and Wilson’s diseases [89,90], might be reduced or prevented by endogenous metal-chelating agents, such as CAR and its homologues [91,92]. Therefore, understanding the role of these endogenous compounds that are able to modulate copper availability and that have putative neuromodulatory and/or neuroprotective actions may help in the development of clinical approaches for the treatment of neuropathologies that involve metals and free radicals.
3. Human Carnosinases
The degradation of carnosine and related peptides has been firstly studied with extracts or partially purified enzyme preparations from porcine [2,93,94,95,96,97], murine [98,99], human  kidneys or rat brain . A cytosolic form previously named tissue carnosinase (EC 184.108.40.206) was first isolated from porcine kidney by Hanson and Smith  in 1949. These authors and Rosenberg [95,96] observed that carnosine-degrading activity in hog kidney extracts was stabilized and activated by Mn2+ ions and strongly inhibited by metal-chelating agents. Later, Lenney et al. [93,94] described two metal-dependent carnosinases from the same material (porcine kidney) which exhibit distinct differences in their substrate specificity. These enzymes apparently do not contain essential sulfhydryl groups since they are not inhibited by p-chloromercuribenzoate, differently from the carnosine-degrading enzyme from brain, reported by Kunze et al. , and from human kidney, described by Lenney et al. .
The authors suggested, however, that “human tissue carnosinase” acts as a cytosolic nonspecific dipeptidase rather than a selective carnosinase based on its broad substrate specificity and the strong inhibition by bestatin . A secreted form of human carnosinase was first described by Perry et al.  in patients with carnosinemia and was first purified from human placenta . The enzyme was also isolated from human plasma and originally named human serum carnosinase (EC 220.127.116.11) . It was distinguished from its cytosolic counterpart because of its particular distribution in human plasma and brain, its unique capability to degrade homocarnosine, and absence in non-primate mammals except for the Syrian golden hamster .
In 2003, Teufel et al.  revealed the nucleotide sequences of two novel genes CNDP1 and CNDP2, coding for human serum carnosinase and human tissue carnosinase (also called cytosolic nonspecific dipeptidase), respectively. The two types of CAR-degrading enzymes (CN1 and CN2) have been identified and characterized in humans and mice [5,105]. Sequence-based alignments of human CN1 and human CN2 with mouse CN2 show sequence identities of 53 and 91%, respectively.
Both purified recombinant enzymes have been characterized for their pH optimum of hydrolysis, substrate specificity, inhibitors, and the effects of metal ions on enzyme activity. The pH activity curve of CN1 protein showed a rather broad maximum between pH 7.5 and 8.5 essentially as previously described for human carnosinase . A narrow pH optimum for carnosine degradation with a maximum around pH 9.5 has been reported for CN2. CN1 resulted insensitive to inhibition by bestatin , indicating that this enzyme represents the previously described serum carnosinase. In contrast, CN2 was sensitive to bestatin inhibition, required Mn2+ and dithiothreitol for enzymatic activity, and was inhibited by Zn2+ [5,105]. The substrate specificity of CN1 has been determined with Xaa-His dipeptides at pH 7.5 (Figure 3). Highest enzyme activity was found with carnosine, and only N-methylcarnosine, Ala-His, Gly-His, and homocarnosine served as substrate for this enzyme. Moreover, non Xaa-His dipeptides, as well as tripeptides containing histidine in central or C-terminal position, were not degraded by CN1 .
The substrate specificity of CN2 protein differed significantly. The enzyme did not hydrolyze carnosine but degraded dipeptides with good activity at pH 7.5 and in presence of 0.1 mM Mn2+. Only under non-physiological conditions (pH 9.5) carnosine served as substrate for CN2, but the relative dipeptidase activity with Xaa-His substrates like Leu-His, Ser-His, or Tyr-His was superior over carnosine degradation. Homocarnosine was not hydrolyzed at all. CN1 activity was completely abolished when three single point mutations (H133A, D166A, E201A), were introduced in CN1 with the aim of removing putative metal binding residues. 1,10-phenantroline also inhibited the enzyme in the low micromolar range .
Whereas at high concentrations (≥100 μM) cadmium activated CN1 protein, at low concentrations (0.1–3 μM) the metal ion strongly inhibited the enzyme. The saturation with Cd2+ ions had a strong effect on both the affinity and turnover of carnosine and homocarnosine degradation in the presence of 0.2 mM Cd2+, the kinetic parameters resulted 10-fold higher for both substrates as compared with conditions where cadmium was omitted. Other metal ions (Fe2+, Al3+, Co2+, Ni2+) with the exception of Cu2+, which strongly inhibited CN1 activity, had no effect on carnosine breakdown.
The selective activation of carnosinase by cadmium was described in the literature  as a criterion to distinguish between the different carnosine-splitting enzymes and can be used as evidence for a two-metal ion co-catalytic mechanism. Although the intrinsically bound metal ions in carnosinase are unknown, it has been suggested carnosinase is associated with Mn2+ and/or Zn2+ based on the homology to other enzymes of the M20 family of metalloproteases . This hypothesis allows to explain why the activity of carnosinase varies in the presence of cadmium. At low cadmium concentrations Mn2+ or Zn2+ in a binding site (site 1) could be replaced with this ion, and catalysis of carnosine degradation is inhibited, whereas enzyme activity is reconstituted if both metal binding sites (1 and 2) are occupied by cadmium ions. Cytosolic non-specific dipeptidase can also be activated by Mn2+, Co2+, and Cd2+, which may explain why in previous studies [4,106] human carnosinase (secreted form) could not be distinguished from the cytosolic nonspecific dipeptidase.
3.1. Biological Activities of Human Carnosinases
The biological function of both enzymes, although not yet well understood, may be quite different, as well. The expression pattern of CN1 differs in humans, mice, and rats: human CN1 is expressed mainly in the brain (particularly in pyramidal cells of the hypocampus) and liver, while CN1 is mainly expressed in the kidney and is not expressed in the brain of mice and rats .
Interestingly, fetal brain does not contain CN1 mRNA suggesting that CN1 gene expression is induced with age. This result explains why carnosinase activity was virtually undetectable in newborn humans and increased with age . CN1 levels increase with age up to about 15 years in serum . It has been also reported an increased expression and activity of carnosinase (CN1) in murine aging brain . In aged animals, CN1 expression was 3-fold higher in substantia nigra compared to all other brain regions examined (cortex, septum, striatum, hippocampus and cerebellum), which showed comparable activities (Figure 4). Senescent rats, as compared to aged, exhibited a significant increase in CN1 expression in all brain regions, but the cortex. Notably, the maximum induction was observed in the substantia nigra and hippocampus, which are brain regions highly vulnerable to oxidant injury and aging effects. This may have important patho-physiological implications, in light of the possibility that an increased expression and activity of CN1, by decreasing carnosine levels can result in a significant decrease in antioxidative potential.
4. Other Aminoacyl-Histidine Dipeptidases
Anserinase was discovered by Jones , who found that anserine (β-alanyl-1-methylhistidine) was hydrolyzed by this enzyme in skeletal muscle of G. callarias. Anserinase is activated by bivalent metal ions and has broad specificity, with ability to hydrolyze many kinds of substrates such as Na-acetylhistidine, N-acetylmethionine, anserine, carnosine, homocarnosine, alanylhistidine, glycyl-leucine and leucylglycine . Previously, it has been reported that this enzyme, purified from the brain of rainbow trout Oncorhynchus mykiss to apparent homogeneity, is a homodimeric protein with a subunit of 55 kDa . It is commonly believed that anserinase is universally distributed in poikilothermic animals containing Na-acetylhistidine in their tissues . Molecular identification of anserinase  revealed that it is a member of the M20 metallopeptidase subfamily, to which CN1 and CN2 belong.
Another two carnosine-cleaving enzymes, peptidase V and peptidase D, are known from bacterial species. Peptidase V belongs to the same peptidase subfamily (M20A) as those of the three above mentioned vertebrate enzymes, while peptidase D (EC. 18.104.22.168) belongs to a different subfamily M20C. Some crystal structure studies of these bacterial peptidases [113,114,115] as well as mouse CN2  have shown that subunits of all the carnosine-cleaving M20 enzymes comprise a catalytic domain containing divalent metal ions including Zn2+ and Mn2+ at active site. Although enzyme activity of CN1 has never been detected in non-mammalian species as stated above, Partmann  reported a carnosine-cleaving enzyme activity in the skeletal muscle of European eel (Anguilla anguilla). No specific information describing the carnosine-cleaving enzyme(s) in eel muscle, however, is available. In fish, it has been known that skeletal muscles of two species of gadids, Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus), are a rich source of Xaa-methyl-His dipeptidase, which can hydrolyze muscle anserine and is originally called ‘anserinase’ . Thereafter, anserinase was demonstrated to have a broad specificity not only for many dipeptides including carnosine but also for several N-acetylated amino acids including N-acetyl-l-His, which is a physiological substrate for the enzyme in fish brain and eye [19,110]. A novel gene named ANSN, coding for anserinase, was identified from Nile tilapia (Oreochromis niloticus) .
Recently , two different carnosine-cleaving enzymes were identified from the skeletal muscle of Japanese eel; one was carnosine dipeptidase I (encoded by CNDP1) having narrow specificity and another was Xaa-methyl-His dipeptidase (encoded by ANSN) having broad specificity. Both genes are strongly expressed in the liver, rather than in the skeletal muscle. This represents the first report on the identification of the dipeptidase encoded by CNDP1 from a non-mammal and may provide valuable insights into the phylogenetic history of the metallopeptidase subfamily M20A in vertebrate evolution (Figure 5).
5. Structural Features of Carnosinases
Based on the similarity in primary sequences, CN1 and CN2 have been classified as metallopeptidases belonging to the M20 family of clanMH. Of these, the crystal structures of PepV from Lactobacillus delbrueckii , CPG2 (carboxypeptidase G2) from Pseudomonas spp.  and PepD from V. alginolyticus [113,121] have been reported until now. The crystal structures of mouse CN2 complexed with bestatin together with Zn2+ and Mn2+ have also been obtained  (Table 1).
|Table 1. Structural and functional properties of several carnosinases.|
|Main substrate||Main Inhibitors|
|CN1||Serum carnosinase||Homo sapiens||Brain, serum, liver||56.8||Cd2+|
|CN2||Cytosolic carnosinase||Homo sapiens||Ubiquitous||52.9||Mn2+||9.5||Xaa-His||Bestatin|
|ANSN||Anserinase||Oncorhynchus mykiss||Brain, eyes||55.0||Co2+, Zn2+||Anserine||Bestatin|
|PepV||Peptidase V||Lactobacillus delbrueckii||Cytoplasm||52.0||Carnosine||Phenantroline|
|PepD||Peptidase D||E. Coli||Cytoplasm||52.9||Co2+||9.0||Xaa-His||Metal chelators|
PepV and CPG2 share 17% and 18% sequence identities with mouse CN2, respectively. Sequence-based alignment of PepD with proteins from the metallopeptidase H Clan shows low sequence identities and similarities in the range of 7%–20% and 13%–34%, respectively [114,115,122,123,124,125,126,127]. Nevertheless, sequence analysis revealed that putative active site residues for catalysis are conserved in PepD and related di-zinc enzymes in the M20 family [114,115].
PepV, PepD and CPG2 are composed of two domains: one catalytic domain with two Zn2+ ions at the active center and one noncatalytic domain known as the lid domain or the dimerization domain. The dimerization domain of PepD and CPG2 provide the surface for the same interactions to form a homodimer structure, whereas PepV is present as a monomer due to the different structural features of the lid domain (Figure 6A). Furthermore, the crystal structure of a member of the M28 family of dinuclear zinc aminopeptidases from Aeromonas proteolytica (AAP)  is similar to the catalytic domain of PepV and CPG2. However, AAP does not have a non-catalytic domain and is present as a monomer in solution (Figure 6B).
Domain A of CN2 is structurally similar to the catalytic domains of PepV, PepD, CPG2, and AAP. This domain provides binding sites for two metal ions and a substrate to form the main part of the active site. Although the overall sequence homology was lower than 20%, metal ion ligands (His99, Asp132, Glu167, Asp195, and His445) and so-called “catalytic Glu” (Glu166) of CN2 were conserved in M20/M28 family proteins, including PepV, CPG2, and AAP, except that Asp195 is substituted with Glu in AAP.
The two metal ions are coordinated octahedrally by domain A residues and bestatin both in the Mn2+ and Zn2+ complexes. Metal 1 is coordinated by an imidazole nitrogen of His445, a carboxylate oxygen of Asp132, two carboxylate oxygens of Glu167, and two backbone carbonyl oxygens of bestatin. On the other hand, Metal 2 is coordinated by a carboxylate oxygen of Asp132, an imidazole nitrogen of His99, and two carboxylate oxygens of Asp195 together with a hydroxyl oxygen and the N-terminal nitrogen of bestatin. Each carboxylate oxygen of Asp132 coordinates to metals 1 and 2, respectively, to form a bridge-like structure between the two metals (Figure 7).
Similar to PepV, CPG2, and AAP, domain A of CN2 contains one cis-peptide bond between Asp132 and Asp133, this bond appears to be necessary to force the metal-bridging carboxylate such that it conforms to the correct geometry.
The metal ion selectivity of CN2 results completely different from those of M28 family metallopeptidases. CN2 requires Mn2+ selectively for its catalytic activity and is not activated by Zn2+, Cu2+, or Mg2+ , whereas all of the M28 family metallopeptidases are activated by Zn2+ . Metal ion selectivity of AAP has been reported in detail; it is activated by Zn2+, Co2+, Ni2+, and Cu2+, whereas it is not activated by Mg2+ or Mn2+ . A comparison of the active center of CN2 with those of M28 metallopeptidase family proteins reveals that the ligand residues and catalytic Glu are located in the same sites. The only remarkable difference between the active centers of CN2 and M28 peptidases is the orientation of the main chain oxygen atom of Asp195. This arrangement of Asp oxygen is unique to CN2 and PepV, whereas it does not exist in any structure of the M28 family reported. In the CN2-bestatin complex, it has a hydrogen bonding interaction with the N-terminal nitrogen atom of bestatin. This suggests that the N-terminal nitrogen of bound carnosine will probably also interact with the oxygen. Furthermore, the different orientation of the main chain oxygen atom of Asp195 could result in a differential selectivity of metal ions in addition to substrate specificity.
In the catalytic domain in PepD  His80, Asp119, Glu150, Asp173, and His461 are involved in metal binding, whereas Asp82 and Glu149 are necessary for catalysis. The N- and C-terminal ends are located on the top of the catalytic domain, opposite to the lid domain and the active site. Optimal activation of apo-PepD has been observed with various divalent metal ions, including Mn2+, Co2+, Ni2+, Cu2+, and Cd2+ . Previous studies have shown that the addition of Co2+ ions to apo-PepD increases the enzymatic activity by a factor of ~1.4, compared with that of the wild-type PepD containing Zn2+. Moreover, Zn2+ did not inhibit Co2+-loaded PepD activity. Substitution of Zn2+ with Mg2+ resulted in an approximate 80% restoration of the optimal enzymatic activity. The di-zinc center is situated on the surface of the cleft between the catalytic and lid domains and, thus, it is solvent-accessible. The crystal structure of PepD also reveals that several functional residues interact and fix two zinc ions (Zn1 and Zn2). Zn1 is coordinated by one of the carboxylate oxygens of Asp119, Nε2 from His461, and a single putative water molecule bound by hydrogen bonding with the carboxylate group of Glu149. Zn2 is coordinated by Nε2 of His80, the other carboxylate oxygen of Asp119, and two carboxylate oxygens of Asp173. Asp119 is positioned as a bridging ligand between the two zinc ions. Notably, this residue is followed by an asparagine residue through a cis peptide bond as observed in many di-zinc-dependent enzymes of the M20/M28 family . In PepV, the carboxylate oxygens of Glu154 point inward to Zn1, whereas in PepD the carboxylate oxygens of Glu150 point away from the Zn1. Thus, the role of metal ion binding for Glu150 in PepD remains ambiguous.
Currently, the crystal structures of the M20 family of proteins have been reported for two different (open and closed) conformations. When the protein is crystallized in a free form, the catalytic and lid domains are in an orientation that exposes the active site to bulky water; whereas when the protein is complexed with an inhibitor, a closed conformation has been observed. The structure of PepV complexed with an inhibitor showed that it has a closed conformation similar to that of CN2 complexed with bestatin .
In the PepV-inhibitor complex, a fixed “bridging” water molecule was found to be located between the two zinc ions and close to the carboxylate group of the catalytic Glu153, which corresponds to Glu149 of PepD and has been proposed as necessary for substrate hydrolysis. Upon binding of the substrate, the water molecule results placed between the zinc ions and the carbonyl residue of the scissile peptide bond. Then, an attacking hydroxyl ion nucleophile is able to be subsequently generated through activation of the water molecule by both the zinc ions and transfer of the resultant proton to the Glu153. Proximal to the Glu153 of PepV is the conserved metal-binding residue, Glu154, which utilizes its carboxylate oxygen to bind to the zinc ion. The carboxylate oxygen of Glu154 of PepV is directed toward the Zn1. Nevertheless, the structural analysis of PepD in an open conformation  revealed that the carboxylate oxygen of the corresponding Glu150 residue of PepD is directed away from the Zn1. It has been suggested that dipeptidases of M20 families can change their conformation from opened to closed during enzymatic catalysis . The conformational change could be achieved by a movement of the catalytic and lid domains, as observed both for the PepD structure  and for the CN2 one . It has been speculated that upon substrate binding, the PepD protein may change the metal ions’ coordination and/or its protein conformation [113,116]; the carboxylate oxygen of Glu150 would be subsequently swung toward Zn1 and would push the Glu149-bound water molecule toward Zn2, effectively bridging the water between the two zinc ions. However, the precise molecular interactions between the enzyme active site and the substrate or inhibitor still await final x-ray structure determination. The di-zinc binding amino acid residues, His80, Asp119, Glu150, and His461, are conserved among all of the proteins compared, but the Asp173 was found to be replaced by Glu in CPG2 and hACy1. This finding is consistent with the observation reported by Lindner et al.  that all homologs with proven aminopeptidase or dipeptidase specificity contain an aspartic acid, whereas a glutamic acid residue has been identified in the same position in Acyl1/M20 family members that exhibit either aminoacylase or carboxypeptidase specificity.
Three residues, Asn217, His269, and Arg350 have been identified , within the lid domain of PepV, that are putatively involved in the substrate C-terminal and/or transition state binding through hydrogen bonding. Due to the different topology of the β-sheet order, a simple primary sequence alignment was not able to identify the corresponding residues in the lid domain of PepD, except for Arg369, which aligned with Arg350 of PepV. This residue also superimposed with Arg324, in the small domains of the dimeric CPG2. A structure-based sequence alignment has then been used to identify the other equivalent residues in PepD. A structure superimposition but inversed sequence order, in which the Asn217 and His269 residues of PepV superimposed with the Asn260 and His219 residues of PepD, has been found. Asn260 is conserved among PepV, CPG2, but is substituted by Thr in human CN1 and mouse CN2. Remarkably, the His219, Asn260, and Arg369 residues are located on the same side of the lid domain for both PepD dimers, whereas the corresponding residues are located on the opposite side of the lid domain of the same monomer for CPG2 and related dimeric proteins. Therefore, the Arg288 from the lid domain of one monomer interacts with the His229 and Asn275 from the lid domain of the other monomer in CPG2; in contrast, the Arg369 from the lid domain of the PepD monomer interacts with the Asn260 and His219 from the lid domain of the same monomer.
Site-directed mutagenesis experiments have been carried out to test the roles of these equivalent lid domain residues. Arg369 to Ala mutation resulted in complete loss of the enzymatic activity for hydrolyzing l-carnosine, whereas the Asn260 to Ala mutation decreased the catalytic activity to almost half. Interestingly, the His219 to Ala mutation did not affect the enzymatic activity significantly, yielding only a slight increase in activity of ~10% as compared with the wild-type PepD. In PepV, Arg350 results placed near the C terminus of the bound inhibitor (2.7 Å) but is too far away from the zinc ions, indicating a role in substrate binding but not in catalysis. The replacement of Arg with Ala might disrupt the hydrogen bond network between the Arg369 side chain and Asn260 Nδ with the carboxylate group of the substrate. In the case of PepV, Jozic et al.  have argued that domain flexibility is required to allow substrate access, in the case of PepV. A significant opening of the protein conformation would clearly benefit access of the peptides to the active site cavity. It is conceivable that even the whole lid domain might move away from its site to allow for easier substrate access and product egress. Therefore, although the Arg369 guanidinium side chain and the Asn260 Nδ within the active site of PepD are both away from the zinc ion, a conformational change between the open and closed states might contribute to the movement of both Arg369 and Asn260 upon substrate binding and subsequent transition state stabilization. Furthermore, binding of the His219 in PepD to the substrate likely persists during the conformational change between the open and closed states and contributes to transition-state stabilization through an electrostatic interaction between His219 and the free carboxyl group of the ligand, as shown in the PepV-inhibitor complex.
Recently, a crystal structure of CN1 complexed with zinc(II) was deposited in the Protein Data Bank. The protein has been crystallized in dimer form. The catalytic domain seems very similar to that reported for other carnosinases, although no paper has been published yet about these data. A Zn2+ ion is coordinated by the imidazole nitrogen of His106, the carboxylate oxygen of Asp202. The other zinc(II) ion is coordinated by a carboxylate oxygen of Glu174, an imidazole nitrogen of His452. Each carboxylate oxygen of Asp139 coordinates to both metal ions to form a bridge-like structure between the two metals (Figure 8).
The structures of carnosinases allow for a better knowledge of the molecular recognition of carnosine by carnosinase which might support the rational design of stable derivatives which conserve the peptide bond. Aldini et al.  suggested a putative complex between carnosine and human serum carnosinase and indicated the key interactions stabilized by the zinc ions with both the carboxylate and the carbonyl oxygen atom (Figure 9). This last contact should polarize the carbonyl group playing an essential catalytic role common of hydrolases. The protonated amino group of carnosine should interact with the carboxylates of Asp116 and Glu451, while the nitrogen atoms of the imidazole ring could elicit H-bonds with Leu254 and Thr424, thus providing a rationale for the specificity of serum carnosinase toward histidine-containing dipeptides and for the lower activity of serum carnosinase toward carnosine analogs with a substituted nitrogen in the imidazole ring, though anserine is, however, hydrolyzed by the enzyme.
6. Carnosinase-Related Diseases
Case studies of carnosinase deficiency have reported symptoms that include progressive mental deficiency (MD), non-progressive mental retardation, developmental delay, spastic paraplegia, seizures, neurosensory hearing loss, retinitis pigmentosa, and progressive dementia [132,133,134]. Reduced activity levels of CNDP1 have been found in other neurological disorders including Parkinson’s disease, multiple sclerosis, and following a cerebrovascular event [135,136]. On the contrary, CNDP2 is overexpressed in the substantia nigra in Parkinson’s disease . Serum carnosinase levels have also been shown to decrease during cardiopulmonary bypass surgery  and it may be a neuroprotective mechanism, as carnosine may reduce neurotoxicity through its antioxidant capacity . Several reasons have been suggested for altered carnosinase levels in healthy humans or with neurological disorders:
damage to carnosinase-producing cells, though no relationship was found between size of the infarct and carnosinase activity ;
genetic factors, as suggested by the fact that deletion distal to 18q21.3 was found in a child with serum carnosinase deficiency  (the gene for serum carnosinase is located on 18q22.3 ); moreover a locus on chromosome 18 was identified for familial AD in Caribbean Hispanics  and an association between the allelic variation of this gene and carnosinase activity has been shown . In this study, patients with MD may have always had low carnosinase activity and then susceptible to dementia.
down-regulation of carnosinase mRNA, as shown for the AD marker neural thread protein (AD7CNTP). It is up regulated in the brains and CSF of patients with AD compared to controls .
regular physical activity, associated with increased carnosinase activity and with a decreased risk of vascular dementia in women .
Many studies have focused on genetic association of CN1 with human diabetic nephropathy in the past several years after CNDP1 had been confirmed as a susceptibility locus [67,147,148,149,150,151,152,153,154,155,156,157,158]. The susceptibility to diabetic nephropathy is strongly associated with a polymorphism in the CNDP1 gene . The CNDP1 polymorphism affects CN1 secretion. A low secretion is associated with smaller numbers (5-Leu) of a Leu-repeat in the signal peptide of human CNDP1. Diabetic patients homozygous for 5-Leu type of CNDP1 are protected against diabetic nephropathy, because carnosine acts as a protective factor against adverse effects of high glucose levels on renal cells. Recently, N-glycosylation was found essential for appropriate secretion and enzyme activity, in addition to the Leu-repeat polymorphism of CN1 .
The physiological mechanism for the protective effect of (CTG)5 homozygosity puts forward that (1) a CNDP1 genetic predisposition leads to low serum carnosinase activity ; (2) low carnosinase activity promotes higher concentrations of circulating carnosine; and (3) high circulating carnosine levels protect against hyperglycemia- induced cytotoxic metabolites resulting from oxidative stress and glycation. Although the association between the CNDP1 polymorphism and diabetic nephropathy have been confirmed in an independent study in European Americans , other studies did not show an association in type 1 diabetic patients  or showed that the association in type 2 diabetic patients is sex specific . Inconsistent findings may be explained by differences in ethnicity  or, alternatively, by assuming that protection from diabetic nephropathy afforded by (CTG)5 homozygosity in CNDP1 may be masked by additional risk haplotypes [152,156].
Furthermore, while the expression of CN1 in transgenic db/db mice produced elevations in the blood glucose and hemoglobin A1c levels and a reduction in the blood insulin level, administration of CAR increased serum fasting insulin levels in db/db mice, and a significant correlation was observed between serum CAR levels and β-cell mass in the pancreas .
Marked alterations of renal carnosine metabolism in diabetic mice and their correlation with CN1 activity have recently been shown . Renal CN1 activity is increased whereas tissue anserine concentrations are reduced ten-fold. Treatment with carnosine normalizes renal CN1 activity and renal anserine concentrations. Moreover, exogenous carnosine lowered blood glucose levels, proteinuria and renal vascular permeability. Increased CN1 activity in the diabetic mice might be the consequence of hyperglycemia due to the poor glucose control, in accordance with the finding that hyperglycemia enhances CN1 secretion and enzyme activity . Carnosine treatment of db/db mice did not affect renal tissue carnosine concentrations, but normalized anserine levels. Because of anserine can be formed by methylation of carnosine via carnosine-N-methyl transferase [20,160], renal anserine increases in carnosine treated mice and accumulates, whereas exogenous carnosine is metabolized to anserine or hydrolyzed by CN1. In carnosine-treated diabetic mice, the decrease of CN1 activity may be due to lowered blood glucose levels that reduced N-glycosylation of CN1  and/or the enzymatic inhibition of CN1 activity by anserine .
As a summary of all the carnosinase-related diseases, most of them are related to the effects that carnosinase overexpression has on the carnosine concentration. For this reason, diseases and disorders induced by carnosine dyshomeostasis (ischemia, neurological diseases, wound healing, ocular diseases, etc.), previously described in this review, may be related to variations of carnosinase levels.
Carnosinase as a Biomarker
Among all the diseases, dementia has emerged as a major clinical, societal and economic problem, especially in industrialized societies. Dementia is also a common feature of many neurodegenerative diseases as Alzheimer’s, Parkinson’s and Huntington’s diseases . Early and accurate diagnosis is desirable, as current therapies are most effective in the early stages and also because it could allow the cognitively aware patient to deal with future issues in medical care, safety, and legal matters.
Taking AD as an example, abundant evidence suggests the existence of a “preclinical” stage that can start 10–15 years before a subject can be diagnosed, where an individual is cognitively normal but is developing extensive pathological changes in the brain, particularly the build-up of amyloid plaques. It will therefore be important to have biomarkers that can identify individuals with preclinical AD or at the earliest clinical stages, in order to prevent or stem the synaptic and neuronal losses associated with cognitive impairment, seen the inadequacy of clinical examination to this purpose.
Numerous plasma and serum markers for dementia have been identified, but they lack the sensitivity and specificity needed for diagnosis and do not allow to monitor the progress of the disease, response to therapy or to predict outcome. Instead, cerebrospinal fluid (CSF) is a potentially rich source for AD biomarkers , as its composition is rapidly and directly influenced by the biochemical changes in the brain.
Indeed, a few CSF biomarkers have already been identified for clinical and even preclinical AD, the most studied proteins being Aβ42 (amyloid β), total tau, and phosphorylated tau species . Among them, human serum carnosinase has also been proposed as a novel biomarker in CSF [163,164]. Although serum carnosinase activity has not shown significant differences between AD and controls, a difference between AD and mixed dementia (including vascular dementia) has been reported . To our knowledge, an evaluation of plasma or CN1 concentrations in the context of AD has not yet been performed or reported. For this reason, further assessment of the potential of these and other proteins as candidate AD biomarkers in plasma or serum remains an important task for future studies.
Very recently, reduced levels of CNDP1 were observed in 8 out of 10 plasma specimens of patients with glioblastoma (GBM) . Carnosine is also reported to have anti-growth property and has been discussed for its therapeutic potential against tumors including GMB . Therefore, reduced levels of serum CNDP1 in the plasma of GMB patients may be important in the maintenance of carnosine levels and bioavailability of the dipeptide as a drug for GBM .
Human serum carnosinase has also been proposed as a novel specific biomarker for ischemic brain tissue damage during and after two different extracorporeal circulation systems . Five minutes after sternum closure, the serum carnosinase activity in the minimized perfusion circuit group remained almost stable and was significantly higher as compared with the conventional circulatory perfusion bypass group, even after correction for hemodilution. This stability is due to a well-preserved oxygen capacity in the brain.
7. Substrate Derivatives of Carnosine and Its Related Dipeptides
A large amount of scientific papers published of carnosine and carnosinase point out the paradox aspect of this biological system, i.e., notwithstanding the beneficial role of endogenous and administrated carnosine, the enzymatic hydrolysis of the dipeptide is very rapid in the serum and in the intracellular environment of some tissues by CN1 and CN2, respectively.
Several attempts have been made to overcome this drawback, but almost all of them could be classified within two main strategies: the β-alanine implementation and the carnosine derivatization.
The first approach is being pursued in the field of the sport medicine with a number of results [167,168,169]. A large number of carnosine derivatives have been synthesized and structurally characterized [130,170].
The main goals reached by the carnosine functionalization have been: (a) avoiding or, at least, reducing the carnosinase hydrolysis; (b) conferring a lipophilic character with the aim to aid the BBB-crossing; (c) enhancing or, at least, maintaining the beneficial effects of the dipeptide; (d) counterbalancing the side effects of the grafted compound and (e) aiming at the targeted delivery.
Carnosine has been conjugated with several molecules, such as carbohydrates (cyclodextrins, trehalose, glucose, lactose, galactose) [34,171,172,173], antioxidants (Trolox, ascorbic acid) [174,175], drugs (l-Dopa) , physiological cofactors (lipoic acid, biotin) [177,178,179] and many aliphatic and aromatic moieties [180,181,182,183].
The carnosinase resistance has been reported for most of all the carnosine conjugates. The effect against oxidative stress, as well as the ability to bind several metal ions, mainly copper(II) and zinc(II), has largely been exploited [34,171,172,184].
One of the newest potential solutions consists in the use of the d-carnosine enantiomer (d-Car). d-Car is not hydrolyzed by the carnosinases and so its concentration could be maintained in the serum . Moreover, it is able to cross the blood-brain barrier and to maintain the same quenching activity of L-carnosine (l-Car) in vitro ; hence, its use has been suggested for the treatment or prevention of oxidative stress-induced disorders [88,185]. Indeed, despite its behavior against oxidative- and nitrosative-induced damages, d-car is less bioavailable than l-Car because it is not recognized by PepT1 of the colon. Thus, as planned for l-Car, increasing attention has recently been paid to the functionalization of d-Car. Several hydrophilic [171,172] and lipophilic  molecules have been conjugated to d-Car with the aim of increasing the bioavailability of d-Car.
Chemical derivatization of homocarnosine and anserine has also been carried out not for enhancing or maintaining the beneficial effects of the dipeptide and aiming at the targeted delivery. HCAR and ANS were grafted on the upper and lower rims of β-cyclodextrin [186,187]. All these glycoside derivatives have higher antioxidant effects than those of the corresponding free histidine-containing dipeptides towards oxidation of human LDL and their copper(II) complexes catalyze the dismutation of superoxide anion (SOD-like activity) . The HCAR-trehalose conjugate  maintains the copper(II) affinity of homocarnosine, shows SOD-like activity, inhibits the action of serum carnosinase on the hydrolysis of carnosine and, interestingly, it also delays the amyloid-β aggregation.
8. Concluding Remarks
Carnosinase and carnosine, as well as other aminoacyl-histidine dipeptidases and imidazole-containing dipeptides, are largely studied in terms of their structure, function and alteration in pathological conditions. Their localization mainly in cerebral tissues has made these molecular systems very attractive for the scientific community because understanding their physiological functions might reasonably be crucial for the development of new strategies to cure or prevent widespread brain-related diseases. The proposal of serum carnosinase as a promising biomarker of AD in CSF  represent an important starting point in this direction.
Diabetes is another important disease in which the carnosine-carnosinase system may play a crucial role. Therefore, CN1 is a potential target for pharmacological interventions aimed at manipulating glucose metabolism. Since only approximately one-third of the entire human population is homozygous for the “low-risk” CNDP1 allele, this approach has the potential for a broad applicability to diabetic patients .The paradox aspect of the carnosine-carnosinase system, i.e., the rapid hydrolysis of the dipeptide in human serum despite the beneficial effects of administrated carnosine well ascertained in physiological and pathological conditions, needs to be overcome and the chemical derivatization has been proposed as a promising strategy to increase the bioavailability of this dipeptide.
We thank MIUR (PRIN 2010MZJARJ, FIRB RINAME RBA-P114AMK and FIRB MERIT RBN-E08HWLZ) for financial support.
Enrico Rizzarelli designed the study and wrote the manuscript together with Francesco Bellia. Graziella Vecchio critically revised it and gave scientific advisory.
Conflicts of Interest
The authors declare no conflict of interest.
- Chen, S.L.; Marino, T.; Fang, W.H.; Russo, N.; Himo, F. Peptide hydrolysis by the binuclear zinc enzyme aminopeptidase from Aeromonas proteolytica: A density functional theory study. J. Phys. Chem. B 2008, 112, 2494–2500. [Google Scholar] [CrossRef]
- Hanson, H.T.; Smith, E.L. Carnosinase: An enzyme of swine kidney. J. Biol. Chem. 1949, 179, 789–801. [Google Scholar]
- Lenney, J.F.; Peppers, S.C.; Kucera-Orallo, C.M.; George, R.P. Characterization of human tissue carnosinase. Biochem. J. 1985, 228, 653–660. [Google Scholar]
- Jackson, M.C.; Kucera, C.M.; Lenney, J.F. Purification and properties of human serum carnosinase. Clin. Chim. Acta 1991, 196, 193–205. [Google Scholar] [CrossRef]
- Teufel, M.; Saudek, V.; Ledig, J.P.; Bernhardt, A.; Boularand, S.; Carreau, A.; Cairns, N.J.; Carter, C.; Cowley, D.J.; Duverger, D.; et al. Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase. J. Biol. Chem. 2003, 278, 6521–6531. [Google Scholar] [CrossRef]
- Gulewitsch, W.; Amiradzibi, S. Carnosine, a new organic base from meat extracts. Ber. Dtsch. Chem. Ges. 1900, 33, 1902–1903. [Google Scholar] [CrossRef]
- Bellia, F.; Vecchio, G.; Cuzzocrea, S.; Calabrese, V.; Rizzarelli, E. Neuroprotective features of carnosine in oxidative driven diseases. Mol. Aspects Med. 2011, 32, 258–266. [Google Scholar] [CrossRef]
- Boldyrev, A.A.; Aldini, G.; Derave, W. Physiology and pathophysiology of carnosine. Physiol. Rev. 2013, 93, 1803–1845. [Google Scholar]
- Bellia, F.; Calabrese, V.; Guarino, F.; Cavallaro, M.; Cornelius, C.; de Pinto, V.; Rizzarelli, E. Carnosinase levels in aging brain: Redox state induction and cellular stress response. Antioxid. Redox Signal. 2009, 11, 2759–2775. [Google Scholar] [CrossRef]
- Gautam, P.; Nair, S.C.; Gupta, M.K.; Sharma, R.; Polisetty, R.V.; Uppin, M.S.; Sundaram, C.; Puligopu, A.K.; Ankathi, P.; Purohit, A.K.; et al. Proteins with altered levels in plasma from glioblastoma patients as revealed by iTRAQ-based quantitative proteomic analysis. PLoS One 2012, 7, e46153. [Google Scholar] [CrossRef]
- Boldyrev, A.A.; Severin, S.E. The histidine-containing dipeptides, carnosine and anserine: Distribution, properties and biological significance. Adv. Enzym. Regul. 1990, 30, 175–194. [Google Scholar] [CrossRef]
- Neidle, A.; Kandera, J. Carnosine--an olfactory bulb peptide. Brain Res. 1974, 80, 359–364. [Google Scholar] [CrossRef]
- Horinishi, H.; Grillo, M.; Margolis, F.L. Purification and characterization of carnosine synthetase from mouse olfactory bulbs. J. Neurochem. 1978, 31, 909–919. [Google Scholar] [CrossRef]
- Kalyankar, G.D.; Meister, A. Enzymatic synthesis of carnosine and related beta-alanyl and gamma-aminobutyryl peptides. J. Biol. Chem. 1959, 234, 3210–3218. [Google Scholar]
- Hoffmann, A.M.; Bakardjiev, A.; Bauer, K. Carnosine-synthesis in cultures of rat glial cells is restricted to oligodendrocytes and carnosine uptake to astrocytes. Neurosci. Lett. 1996, 215, 29–32. [Google Scholar] [CrossRef]
- Drozak, J.; Veiga-da-Cunha, M.; Vertommen, D.; Stroobant, V.; van Schaftingen, E. Molecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1). J. Biol. Chem. 2010, 285, 9346–9356. [Google Scholar]
- Yamada, S.; Tanaka, Y.; Furuichi, M. Partial purification and characterization of histidine acetyltransferase in brain of Nile tilapia (Oreochromis niloticus). Biochim. Biophys. Acta 1995, 1245, 239–247. [Google Scholar] [CrossRef]
- Baslow, M.H.; Lenney, J.F. Alpha-N-acetyl-l-histidine amidohydrolase activity from the brain of the skipjack tuna Katsuwonus pelamis. Can. J. Biochem. 1967, 45, 337–340. [Google Scholar] [CrossRef]
- Lenney, J.F.; Baslow, M.H.; Sugiyama, G.H. Similarity of tuna N-acetylhistidine deacetylase and cod fish anserinase. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 1978, 61, 253–258. [Google Scholar] [CrossRef]
- Drozak, J.; Chrobok, L.; Poleszak, O.; Jagielski, A.K.; Derlacz, R. Molecular identification of carnosine N-methyltransferase as chicken histamine N-methyltransferase-like protein (HNMT-Like). PLoS One 2013, 8, e64805. [Google Scholar]
- Gjessing, L.R.; Lunde, H.A.; Mørkrid, L.; Lenney, J.F.; Sjaastad, O. Inborn errors of carnosine and homocarnosine metabolism. J. Neural Transm. Suppl. 1990, 29, 91–106. [Google Scholar]
- Petroff, O.A.C. GABA and glutamate in the human brain. Neuroscientist 2002, 8, 562–573. [Google Scholar] [CrossRef]
- Petroff, O.A.C.; Hyder, F.; Rothman, D.L.; Mattson, R.H. Homocarnosine and seizure control in juvenile myoclonic epilepsy and complex partial seizures. Neurology 2001, 56, 709–715. [Google Scholar]
- Gil-Agusti, M.; Esteve-Romero, J.; Carda-Broch, S. Anserine and carnosine determination in meat samples by pure micellar liquid chromatography. J. Chromatogr. A 2008, 1189, 444–450. [Google Scholar]
- Baguet, A.; Reyngoudt, H.; Pottier, A.; Everaert, I.; Callens, S.; Achten, E.; Derave, W. Carnosine loading and washout in human skeletal muscles. J. Appl. Physiol. 2009, 106, 837–842. [Google Scholar] [CrossRef]
- Kim, H.J. Comparison of the carnosine and taurine contents of vastus lateralis of elderly Korean males, with impaired glucose tolerance, and young elite Korean swimmers. Amino Acids 2009, 36, 359–363. [Google Scholar] [CrossRef]
- Stuerenburg, H.J.; Kunze, K. Concentrations of free carnosine (a putative membrane-protective antioxidant) in human muscle biopsies and rat muscles. Arch. Gerontol. Geriatr. 1999, 29, 107–113. [Google Scholar] [CrossRef]
- Tallon, M.J.; Harris, R.C.; Maffulli, N.; Tarnopolsky, M.A. Carnosine, taurine and enzyme activities of human skeletal muscle fibres from elderly subjects with osteoarthritis and young moderately active subjects. Biogerontology 2007, 8, 129–137. [Google Scholar] [CrossRef]
- Harris, R.C.; Tallon, M.J.; Dunnett, M.; Boobis, L.; Coakley, J.; Kim, H.J.; Fallowfield, J.L.; Hill, C.A.; Sale, C.; Wise, J.A. The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids 2006, 30, 279–289. [Google Scholar] [CrossRef]
- Smith, E.C. The buffering of muscle in rigor; protein, phosphate and carnosine. J. Physiol. 1938, 92, 336–343. [Google Scholar]
- Aruoma, O.I.; Laughton, M.J.; Halliwell, B. Carnosine, homocarnosine and anserine: Could they act as antioxidants in vivo? Biochem. J. 1989, 264, 863–869. [Google Scholar]
- Kohen, R.; Misgav, R.; Ginsburg, I. The SOD like activity of copper:carnosine, copper:anserine and copper:homocarnosine complexes. Free Radic. Res. Commun. 1991, 12, 179–185. [Google Scholar] [CrossRef]
- Baran, E.J. Metal complexes of carnosine. Biochemistry (Moscow) 2000, 65, 789–797. [Google Scholar]
- Bonomo, R.P.; Bruno, V.; Conte, E.; de Guidi, G.; la Mendola, D.; Maccarrone, G.; Nicoletti, F.; Rizzarelli, E.; Sortino, S.; Vecchio, G. Potentiometric, spectroscopic and antioxidant activity studies of SOD mimics containing carnosine. Dalton Trans. 2003, 4406–4415. [Google Scholar] [CrossRef]
- Cuzzocrea, S.; Genovese, T.; Failla, M.; Vecchio, G.; Fruciano, M.; Mazzon, E.; di Paola, R.; Muia, C.; la Rosa, C.; Crimi, N.; et al. Protective effect of orally administered carnosine on bleomycin-induced lung injury. Am. J. Physiol. Lung Cell. Mol. Physiol. 2007, 292, L1095–L1104. [Google Scholar]
- Chuang, C.H.; Hu, M.L. L-carnosine inhibits metastasis of SK-Hep-1 cells by inhibition of matrix metaoproteinase-9 expression and induction of an antimetastatic gene, nm23-H1. Nutr. Cancer 2008, 60, 526–533. [Google Scholar] [CrossRef]
- Hipkiss, A.R.; Michaelis, J.; Syrris, P. Non-enzymatic glycosylation of the dipeptide L-carnosine, a potential anti-protein-cross-linking agent. FEBS Lett. 1995, 371, 81–85. [Google Scholar]
- Hipkiss, A.R. Carnosine and protein carbonyl groups: A possible relationship. Biochemistry (Moscow) 2000, 65, 771–778. [Google Scholar]
- Lee, Y.T.; Hsu, C.C.; Lin, M.H.; Liu, K.S.; Yin, M.C. Histidine and carnosine delay diabetic deterioration in mice and protect human low density lipoprotein against oxidation and glycation. Eur. J. Pharmacol. 2005, 513, 145–150. [Google Scholar]
- Sauerhofer, S.; Yuan, G.; Braun, G.S.; Deinzer, M.; Neumaier, M.; Gretz, N.; Floege, J.; Kriz, W.; van der Woude, F.; Moeller, M.J. L-carnosine, a substrate of carnosinase-1, influences glucose metabolism. Diabetes 2007, 56, 2425–2432. [Google Scholar]
- Biffo, S.; DeLucia, R.; Mulatero, B.; Margolis, F.; Fasolo, A. Carnosine-, calcitonin gene-related peptide- and tyrosine hydroxylase-immunoreactivity in the mouse olfactory bulb following peripheral denervation. Brain Res. 1990, 528, 353–357. [Google Scholar]
- Bonfanti, L.; Peretto, P.; de Marchis, S.; Fasolo, A. Carnosine-related dipeptides in the mammalian brain. Prog. Neurobiol. 1999, 59, 333–353. [Google Scholar] [CrossRef]
- Xiang, J.; Hu, Y.; Smith, D.E.; Keep, R.F. PEPT2-mediated transport of 5-aminolevulinic acid and carnosine in astrocytes. Brain Res. 2006, 1122, 18–23. [Google Scholar] [CrossRef]
- Hipkiss, A.R. Glycation, ageing and carnosine: Are carnivorous diets beneficial? Mech. Ageing Dev. 2005, 126, 1034–1039. [Google Scholar] [CrossRef]
- Huang, Y.; Duan, J.; Chen, H.; Chen, M.; Chen, G. Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis 2005, 26, 593–599. [Google Scholar] [CrossRef]
- Hipkiss, A.R. Could carnosine suppress zinc-mediated proteasome inhibition and neurodegeneration? Therapeutic potential of a non-toxic but non-patentable dipeptide. Biogerontology 2005, 6, 147–149. [Google Scholar] [CrossRef]
- Hipkiss, A.R. Could carnosine or related structures suppress Alzheimer’s disease? J. Alzheimers Dis. 2007, 11, 229–240. [Google Scholar]
- La Mendola, D.; Sortino, S.; Vecchio, G.; Rizzarelli, E. Synthesis of new carnosine derivatives of b-cyclodextrin and their hydroxyl radical scavenger ability. Helv. Chim. Acta 2002, 85, 1633–1643. [Google Scholar] [CrossRef]
- Fontana, M.; Pinnen, F.; Lucente, G.; Pecci, L. Prevention of peroxynitrite-dependent damage by carnosine and related sulphonamido pseudodipeptides. Cell. Mol. Life Sci. 2002, 59, 546–551. [Google Scholar] [CrossRef]
- Severina, I.S.; Bussygina, O.G.; Pyatakova, N.V. Carnosine as a regulator of soluble guanylate cyclase. Biochemistry (Moscow) 2000, 65, 783–788. [Google Scholar]
- Dukic-Stefanovic, S.; Schinzel, R.; Riederer, P.; Munch, G. AGES in brain ageing: AGE-inhibitors as neuroprotective and anti-dementia drugs? Biogerontology 2001, 2, 19–34. [Google Scholar] [CrossRef]
- Pubill, D.; Verdaguer, E.; Sureda, F.X.; Camins, A.; Pallas, M.; Camarasa, J.; Escubedo, E. Carnosine prevents methamphetamine-induced gliosis but not dopamine terminal loss in rats. Eur. J. Pharmacol. 2002, 448, 165–168. [Google Scholar] [CrossRef]
- Dobrota, D.; Fedorova, T.; Stvolinsky, S.; Babusikova, E.; Likavcanova, K.; Drgova, A.; Strapkova, A.; Boldyrev, A. Carnosine protects the brain of rats and Mongolian gerbils against ischemic injury: After-stroke-effect. Neurochem. Res. 2005, 30, 1283–1288. [Google Scholar]
- Stvolinsky, S.; Kukley, M.; Dobrota, D.; Mezesova, V.; Boldyrev, A. Carnosine protects rats under global ischemia. Brain Res. Bull. 2000, 53, 445–448. [Google Scholar] [CrossRef]
- Tang, S.C.; Arumugam, T.V.; Cutler, R.G.; Jo, D.G.; Magnus, T.; Chan, S.L.; Mughal, M.R.; Telljohann, R.S.; Nassar, M.; Ouyang, X.; et al. Neuroprotective actions of a histidine analogue in models of ischemic stroke. J. Neurochem. 2007, 101, 729–736. [Google Scholar] [CrossRef]
- Calabrese, V.; Colombrita, C.; Guagliano, E.; Sapienza, M.; Ravagna, A.; Cardile, V.; Scapagnini, G.; Santoro, A.M.; Mangiameli, A.; Butterfield, D.A.; et al. Protective effect of carnosine during nitrosative stress in astroglial cell cultures. Neurochem. Res. 2005, 30, 797–807. [Google Scholar]
- Calabrese, V.; Mancuso, C.; Calvani, M.; Rizzarelli, E.; Butterfield, D.A.; Stella, A.M. Nitric oxide in the central nervous system: Neuroprotection versus neurotoxicity. Nat. Rev. Neurosci. 2007, 8, 766–775. [Google Scholar] [CrossRef]
- Nicoletti, V.G.; Santoro, A.M.; Grasso, G.; Vagliasindi, L.I.; Giuffrida, M.L.; Cuppari, C.; Purrello, V.S.; Stella, A.M.; Rizzarelli, E. Carnosine interaction with nitric oxide and astroglial cell protection. J. Neurosci. Res. 2007, 85, 2239–2245. [Google Scholar]
- Preston, J.E.; Hipkiss, A.R.; Himsworth, D.T.; Romero, I.A.; Abbott, J.N. Toxic effects of beta-amyloid(25–35) on immortalised rat brain endothelial cell: Protection by carnosine, homocarnosine and beta-alanine. Neurosci. Lett. 1998, 242, 105–108. [Google Scholar] [CrossRef]
- Attanasio, F.; Convertino, M.; Magno, A.; Caflisch, A.; Corazza, A.; Haridas, H.; Esposito, G.; Cataldo, S.; Pignataro, B.; Milardi, D.; et al. Carnosine inhibits Abeta(42) aggregation by perturbing the H-bond network in and around the central hydrophobic cluster. ChemBioChem 2013, 14, 583–592. [Google Scholar] [CrossRef]
- Hipkiss, A.R.; Preston, J.E.; Himsworth, D.T.; Worthington, V.C.; Keown, M.; Michaelis, J.; Lawrence, J.; Mateen, A.; Allende, L.; Eagles, P.A.; et al. Pluripotent protective effects of carnosine, a naturally occurring dipeptide. Ann. N. Y. Acad. Sci. 1998, 854, 37–53. [Google Scholar] [CrossRef]
- Fonteh, A.N.; Harrington, R.J.; Tsai, A.; Liao, P.; Harrington, M.G. Free amino acid and dipeptide changes in the body fluids from Alzheimer’s disease subjects. Amino Acids 2007, 32, 213–224. [Google Scholar] [CrossRef]
- Jin, C.L.; Yang, L.X.; Wu, X.H.; Li, Q.; Ding, M.P.; Fan, Y.Y.; Zhang, W.P.; Luo, J.H.; Chen, Z. Effects of carnosine on amygdaloid-kindled seizures in Sprague-Dawley rats. Neuroscience 2005, 135, 939–947. [Google Scholar] [CrossRef]
- Pegova, A.; Abe, H.; Boldyrev, A. Hydrolysis of carnosine and related compounds by mammalian carnosinases. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 2000, 127, 443–446. [Google Scholar] [CrossRef]
- Hou, W.C.; Chen, H.J.; Lin, Y.H. Antioxidant peptides with Angiotensin converting enzyme inhibitory activities and applications for Angiotensin converting enzyme purification. J. Agric. Food Inf. 2003, 51, 1706–1709. [Google Scholar] [CrossRef]
- Aydogan, S.; Yapislar, H.; Artis, S.; Aydogan, B. Impaired erythrocytes deformability in H(2)O(2)-induced oxidative stress: Protective effect of L-carnosine. Clin. Hemorheol. Microcirc. 2008, 39, 93–98. [Google Scholar]
- Janssen, B.; Hohenadel, D.; Brinkkoetter, P.; Peters, V.; Rind, N.; Fischer, C.; Rychlik, I.; Cerna, M.; Romzova, M.; de Heer, E.; et al. Carnosine as a protective factor in diabetic nephropathy: Association with a leucine repeat of the carnosinase gene CNDP1. Diabetes 2005, 54, 2320–2327. [Google Scholar] [CrossRef]
- Tanida, M.; Shen, J.; Kubomura, D.; Nagai, K. Effects of anserine on the renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats. Physiol. Res. 2010, 59, 177–185. [Google Scholar]
- Kubomura, D.; Matahira, Y.; Nagai, K.; Niijima, A. Effect of anserine ingestion on hyperglycemia and the autonomic nerves in rats and humans. Nutr. Neurosci. 2010, 13, 183–188. [Google Scholar] [CrossRef]
- Suzuki, Y.; Nakao, T.; Maemura, H.; Sato, M.; Kamahara, K.; Morimatsu, F.; Takamatsu, K. Carnosine and anserine ingestion enhances contribution of nonbicarbonate buffering. Med. Sci. Sports Exercise 2006, 38, 334–338. [Google Scholar]
- Szwergold, B.S. Carnosine and anserine act as effective transglycating agents in decomposition of aldose-derived Schiff bases. Biochem. Biophys. Res. Commun. 2005, 336, 36–41. [Google Scholar] [CrossRef]
- Boldyrev, A.; Bulygina, E.; Leinsoo, T.; Petrushanko, I.; Tsubone, S.; Abe, H. Protection of neuronal cells against reactive oxygen species by carnosine and related compounds. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 2004, 137, 81–88. [Google Scholar] [CrossRef]
- Kang, J.H.; Kim, K.S.; Choi, S.Y.; Kwon, H.Y.; Won, M.H.; Kang, T.C. Protective effects of carnosine, homocarnosine and anserine against peroxyl radical-mediated Cu,Zn-superoxide dismutase modification. Biochim. Biophys. Acta 2002, 1570, 89–96. [Google Scholar]
- Aldini, G.; Facino, R.M.; Beretta, G.; Carini, M. Carnosine and related dipeptides as quenchers of reactive carbonyl species: From structural studies to therapeutic perspectives. BioFactors 2005, 24, 77–87. [Google Scholar] [CrossRef]
- Min, J.; Senut, M.C.; Rajanikant, K.; Greenberg, E.; Bandagi, R.; Zemke, D.; Mousa, A.; Kassab, M.; Farooq, M.U.; Gupta, R.; et al. Differential neuroprotective effects of carnosine, anserine, and N-acetyl carnosine against permanent focal ischemia. J. Neurosci. Res. 2008, 86, 2984–2991. [Google Scholar]
- Sadzuka, Y.; Sonobe, T. Anserine induced advantage effects on the antitumor activity of doxorubicin. Food Chem. Toxicol. 2007, 45, 985–989. [Google Scholar] [CrossRef]
- Shao, L.; Li, Q.H.; Tan, Z. L-carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts. Biochem. Biophys. Res. Commun. 2004, 324, 931–936. [Google Scholar] [CrossRef]
- Boldyrev, A.A.; Dupin, A.M.; Bunin, A.; Babizhaev, M.A.; Severin, S.E. The antioxidative properties of carnosine, a natural histidine containing dipeptide. Biochem. Int. 1987, 15, 1105–1113. [Google Scholar]
- Babizhayev, M.A.; Kasus-Jacobi, A. State of the art clinical efficacy and safety evaluation of N-acetylcarnosine dipeptide ophthalmic prodrug. Principles for the delivery, self-bioactivation, molecular targets and interaction with a highly evolved histidyl-hydrazide structure in the treatment and therapeutic management of a group of sight-threatening eye diseases. Curr. Clin. Pharmacol. 2009, 4, 4–37. [Google Scholar] [CrossRef]
- Babizhayev, M.A.; Yegorov, Y.E. Telomere attrition in lens epithelial cells - a target for N-acetylcarnosine therapy. Front. Biosci. 2010, 15, 934–956. [Google Scholar] [CrossRef]
- Katayama, S.; Nishizawa, K.; Hirano, M.; Yamamura, S.; Momose, Y. Effect of polaprezinc on healing of acetic acid-induced stomatitis in hamsters. J. Pharm. Pharm. Sci. 2000, 3, 114–117. [Google Scholar]
- Matsukura, T.; Tanaka, H. Applicability of zinc complex of L-carnosine for medical use. Biochemistry (Moscow) 2000, 65, 817–823. [Google Scholar]
- Ueda, K.; Ueyama, T.; Oka, M.; Ito, T.; Tsuruo, Y.; Ichinose, M. Polaprezinc (Zinc L-carnosine) is a potent inducer of anti-oxidative stress enzyme, heme oxygenase (HO)-1 - a new mechanism of gastric mucosal protection. J. Pharmacol. Sci. 2009, 110, 285–294. [Google Scholar] [CrossRef]
- Odashima, M.; Otaka, M.; Jin, M.; Konishi, N.; Sato, T.; Kato, S.; Matsuhashi, T.; Nakamura, C.; Watanabe, S. Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine. Dig. Dis. Sci. 2002, 47, 2799–2804. [Google Scholar] [CrossRef]
- Ohata, S.; Moriyama, C.; Yamashita, A.; Nishida, T.; Kusumoto, C.; Mochida, S.; Minami, Y.; Nakada, J.; Shomori, K.; Inagaki, Y.; et al. Polaprezinc Protects Mice against Endotoxin Shock. J. Clin. Biochem. Nutr. 2010, 46, 234–243. [Google Scholar] [CrossRef]
- Kalmar, B.; Greensmith, L. Induction of heat shock proteins for protection against oxidative stress. Adv. Drug Del. Rev. 2009, 61, 310–318. [Google Scholar] [CrossRef]
- Corona, C.; Frazzini, V.; Silvestri, E.; Lattanzio, R.; la Sorda, R.; Piantelli, M.; Canzoniero, L.M.; Ciavardelli, D.; Rizzarelli, E.; Sensi, S.L. Effects of dietary supplementation of carnosine on mitochondrial dysfunction, amyloid pathology, and cognitive deficits in 3xTg-AD mice. PLoS One 2011, 6, e17971. [Google Scholar] [CrossRef]
- Di Paola, R.; Impellizzeri, D.; Salinaro, A.T.; Mazzon, E.; Bellia, F.; Cavallaro, M.; Cornelius, C.; Vecchio, G.; Calabrese, V.; Rizzarelli, E.; et al. Administration of carnosine in the treatment of acute spinal cord injury. Biochem. Pharmacol. 2011, 82, 1478–1489. [Google Scholar] [CrossRef]
- Barnham, K.J.; Bush, A.I. Metals in Alzheimer’s and Parkinson’s diseases. Curr. Opin. Chem. Biol. 2008, 12, 222–228. [Google Scholar] [CrossRef]
- Di Donato, M.; Hsu, H.F.; Narindrasorasak, S.; Que, L., Jr.; Sarkar, B. Copper-induced conformational changes in the N-terminal domain of the Wilson disease copper-transporting ATPase. Biochemistry (Moscow) 2000, 39, 1890–1896. [Google Scholar] [CrossRef]
- Fu, Q.; Dai, H.; Hu, W.; Fan, Y.; Shen, Y.; Zhang, W.; Chen, Z. Carnosine protects against Abeta42-induced neurotoxicity in differentiated rat PC12 cells. Cell. Mol. Neurobiol. 2008, 28, 307–316. [Google Scholar] [CrossRef]
- Trombley, P.Q.; Horning, M.S.; Blakemore, L.J. Interactions between carnosine and zinc and copper: Implications for neuromodulation and neuroprotection. Biochemistry (Moscow) 2000, 65, 807–816. [Google Scholar]
- Lenney, J.F. Specificity and distribution of mammalian carnosinase. Biochim. Biophys. Acta 1976, 429, 214–219. [Google Scholar] [CrossRef]
- Lenney, J.F.; Kan, S.C.; Siu, K.; Sugiyama, G.H. Homocarnosinase: A hog kidney dipeptidase with a broader specificity than carnosinase. Arch. Biochem. Biophys. 1977, 184, 257–266. [Google Scholar] [CrossRef]
- Rosenberg, A. The activation of carnosinase by divalent metal ions. Biochim. Biophys. Acta 1960, 45, 297–316. [Google Scholar] [CrossRef]
- Rosenberg, A. Purification and some properties of carnosinase of swine kidney. Arch. Biochem. Biophys. 1960, 88, 83–93. [Google Scholar] [CrossRef]
- Wolos, A.; Piekarska, K.; Glogowski, J.; Konieczka, I. Two molecular forms of swine kidney carnosinase. Int. J. Biochem. 1978, 9, 57–62. [Google Scholar] [CrossRef]
- Margolis, F.L.; Grillo, M.; Brown, C.E. Enzymatic and immunological evidence for two forms of carnosinase in the mouse. Biochim. Biophys. Acta 1979, 570, 311–323. [Google Scholar] [CrossRef]
- Margolis, F.L.; Grillo, M.; Grannot Reisfeld, N.; Farbman, A.I. Purification, characterization and immunocytochemical localization of mouse kidney carnosinase. Biochim. Biophys. Acta Protein Struct. Mol. Enzymol. 1983, 744, 237–248. [Google Scholar] [CrossRef]
- Kunze, N.; Kleinkauf, H.; Bauer, K. Characterization of two carnosine-degrading enzymes from rat brain. Partial purification and characterization of a carnosinase and a beta-alanyl-arginine hydrolase. Eur. J. Biochem. 1986, 160, 605–613. [Google Scholar] [CrossRef]
- Lenney, J.F. Human cytosolic carnosinase: Evidence of identity with prolinase, a non-specific dipeptidase. Biol. Chem. Hoppe-Seyler 1990, 371, 167–171. [Google Scholar] [CrossRef]
- Perry, T.L.; Hansen, S.; Love, D.L. Serum-carnosinase deficiency in carnosinaemia. Lancet 1968, 1, 1229–1230. [Google Scholar] [CrossRef]
- Zoch, E.; Muller, H. Demonstration and determination of earnosinase activity of the human placenta. Enzymologia 1971, 40, 199–208. [Google Scholar]
- Lenney, J.F.; George, R.P.; Weiss, A.M.; Kucera, C.M.; Chan, P.W.; Rinzler, G.S. Human serum carnosinase: Characterization, distinction from cellular carnosinase, and activation by cadmium. Clin. Chim. Acta 1982, 123, 221–231. [Google Scholar] [CrossRef]
- Otani, H.; Okumura, N.; Hashida-Okumura, A.; Nagai, K. Identification and characterization of a mouse dipeptidase that hydrolyzes L-carnosine. J. Biochem. 2005, 137, 167–175. [Google Scholar] [CrossRef]
- Murphey, W.H.; Patchen, L.; Lindmark, D.G. Carnosinase: A fluorometric assay and demonstration of two electrophoretic forms in human tissue extracts. Clin. Chim. Acta 1972, 42, 309–314. [Google Scholar] [CrossRef]
- Pandya, V.; Ekka, M.K.; Dutta, R.K.; Kumaran, S. Mass spectrometry assay for studying kinetic properties of dipeptidases: Characterization of human and yeast dipeptidases. Anal. Biochem. 2011, 418, 134–142. [Google Scholar] [CrossRef]
- Bando, K.; Shimotsuji, T.; Toyoshima, H. Fluorometric assay of human serum carnosinase activity in normal children, adults and patients with myopathy. Ann. Clin. Biochem. 1984, 21, 510–514. [Google Scholar] [CrossRef]
- Jones, N.R. The free amino acids of fish; 1-methylhistidine and beta-alanine liberation by skeletal muscle anserinase of codling (Gadus callarias). Biochem. J. 1955, 60, 81–87. [Google Scholar]
- Yamada, S.; Tanaka, Y.; Sameshima, M.; Furuichi, M. Properties of Nα-acetylhistidine deacetylase in brain of rainbow trout Oncorhynchus mykiss. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 1993, 106, 309–315. [Google Scholar] [CrossRef]
- Yamada, S.; Tanaka, Y.; Sameshima, M.; Furuichi, M. Effects of starvation and feeding on tissue Nα -acetylhistidine levels in Nile tilapia Oreochromis niloticus. Comp. Biochem. Physiol. A: Physiol. 1994, 109, 277–283. [Google Scholar] [CrossRef]
- Yamada, S.; Tanaka, Y.; Ando, S. Purification and sequence identification of anserinase. FEBS J. 2005, 272, 6001–6013. [Google Scholar] [CrossRef]
- Chang, C.Y.; Hsieh, Y.C.; Wang, T.Y.; Chen, Y.C.; Wang, Y.K.; Chiang, T.W.; Chen, Y.J.; Chang, C.H.; Chen, C.J.; Wu, T.K. Crystal structure and mutational analysis of aminoacylhistidine dipeptidase from Vibrio alginolyticus reveal a new architecture of M20 metallopeptidases. J. Biol. Chem. 2010, 285, 39500–39510. [Google Scholar] [CrossRef]
- Jozic, D.; Bourenkow, G.; Bartunik, H.; Scholze, H.; Dive, V.; Henrich, B.; Huber, R.; Bode, W.; Maskos, K. Crystal structure of the dinuclear zinc aminopeptidase PepV from Lactobacillus delbrueckii unravels its preference for dipeptides. Structure 2002, 10, 1097–1106. [Google Scholar] [CrossRef]
- Rowsell, S.; Pauptit, R.A.; Tucker, A.D.; Melton, R.G.; Blow, D.M.; Brick, P. Crystal structure of carboxypeptidase G2, a bacterial enzyme with applications in cancer therapy. Structure 1997, 5, 337–347. [Google Scholar] [CrossRef]
- Unno, H.; Yamashita, T.; Ujita, S.; Okumura, N.; Otani, H.; Okumura, A.; Nagai, K.; Kusunoki, M. Structural basis for substrate recognition and hydrolysis by mouse carnosinase CN2. J. Biol. Chem. 2008, 283, 27289–27299. [Google Scholar]
- Partmann, W. Eine Carnosine spaltende Enzymaktivität im Skelettmuskel des Aales. Arch. Fisch Wiss. 1976, 27, 55–62. [Google Scholar]
- Perez, B.S.; Jones, N.R. Effects of tetracycline antibiotics on the products of anserinase action in chill stored haddock (Gadus aeglefinus) muscle. J. Food Sci. 1962, 27, 69–72. [Google Scholar] [CrossRef]
- Oku, T.; Ando, S.; Tsai, H.C.; Yamashita, Y.; Ueno, H.; Shiozaki, K.; Nishi, R.; Yamada, S. Purification and identification of two carnosine-cleaving enzymes, carnosine dipeptidase I and Xaa-methyl-His dipeptidase, from Japanese eel (Anguilla japonica). Biochimie 2012, 94, 1281–1290. [Google Scholar] [CrossRef]
- Vongerichten, K.F.; Klein, J.R.; Matern, H.; Plapp, R. Cloning and nucleotide sequence analysis of pepV, a carnosinase gene from Lactobacillus delbrueckii subsp. lactis DSM 7290, and partial characterization of the enzyme. Microbiology 1994, 140, 2591–2600. [Google Scholar] [CrossRef]
- Chang, C.Y.; Hsieh, Y.C.; Wang, T.Y.; Chen, C.J.; Wu, T.K. Purification, crystallization and preliminary X-ray analysis of an aminoacylhistidine dipeptidase (PepD) from Vibrio alginolyticus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2009, 65, 216–218. [Google Scholar] [CrossRef]
- Chevrier, B.; Schalk, C.; D’Orchymont, H.; Rondeau, J.M.; Moras, D.; Tarnus, C. Crystal structure of Aeromonas proteolytica aminopeptidase: A prototypical member of the co-catalytic zinc enzyme family. Structure 1994, 2, 283–291. [Google Scholar] [CrossRef]
- Greenblatt, H.M.; Almog, O.; Maras, B.; Spungin-Bialik, A.; Barra, D.; Blumberg, S.; Shoham, G. Streptomyces griseus aminopeptidase: X-ray crystallographic structure at 1.75 A resolution. J. Mol. Biol. 1997, 265, 620–636. [Google Scholar] [CrossRef]
- Hakansson, K.; Miller, C.G. Structure of peptidase T from Salmonella typhimurium. Eur. J. Biochem. 2002, 269, 443–450. [Google Scholar] [CrossRef]
- Lindner, H.A.; Lunin, V.V.; Alary, A.; Hecker, R.; Cygler, M.; Menard, R. Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family. J. Biol. Chem. 2003, 278, 44496–44504. [Google Scholar]
- Lundgren, S.; Gojkovic, Z.; Piskur, J.; Dobritzsch, D. Yeast beta-alanine synthase shares a structural scaffold and origin with dizinc-dependent exopeptidases. J. Biol. Chem. 2003, 278, 51851–51862. [Google Scholar]
- Wang, T.Y.; Chen, Y.C.; Kao, L.W.; Chang, C.Y.; Wang, Y.K.; Liu, Y.H.; Feng, J.M.; Wu, T.K. Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus. FEBS J. 2008, 275, 5007–5020. [Google Scholar] [CrossRef]
- Rawlings, N.D.; Barrett, A.J. Evolutionary families of metallopeptidases. Methods Enzymol. 1995, 248, 183–228. [Google Scholar] [CrossRef]
- Bayliss, M.E.; Prescott, J.M. Modified activity of Aeromonas aminopeptidase: Metal ion substitutions and role of substrates. Biochemistry (Moscow) 1986, 25, 8113–8117. [Google Scholar] [CrossRef]
- Vistoli, G.; Carini, M.; Aldini, G. Transforming dietary peptides in promising lead compounds: The case of bioavailable carnosine analogs. Amino Acids 2012, 43, 111–126. [Google Scholar] [CrossRef]
- Vistoli, G.; Pedretti, A.; Cattaneo, M.; Aldini, G.; Testa, B. Homology modeling of human serum carnosinase, a potential medicinal target, and MD simulations of its allosteric activation by citrate. J. Med. Chem. 2006, 49, 3269–3277. [Google Scholar] [CrossRef]
- Cohen, M.; Hartlage, P.L.; Krawiecki, N. Serum carnosinase deficiency: A non-disabling phenotype? J. Ment. Defic. Res. 1985, 29, 383–389. [Google Scholar]
- Lunde, H.A.; Gjessing, L.R.; Sjaastad, O. Homocarnosinosis: Influence of dietary restriction of histidine. Neurochem. Res. 1986, 11, 825–838. [Google Scholar] [CrossRef]
- Balion, C.M.; Benson, C.; Raina, P.S.; Papaioannou, A.; Patterson, C.; Ismaila, A.S. Brain type carnosinase in dementia: A pilot study. BMC Neurol. 2007, 7, 38. [Google Scholar] [CrossRef]
- Butterworth, R.J.; Wassif, W.S.; Sherwood, R.A.; Gerges, A.; Poyser, K.H.; Garthwaite, J.; Peters, T.J.; Bath, P.M.W. Serum neuron-specific enolase, carnosinase, and their ratio in acute stroke: An enzymatic test for predicting outcome? Stroke 1996, 27, 2064–2068. [Google Scholar] [CrossRef]
- Wassif, W.S.; Sherwood, R.A.; Amir, A.; Idowu, B.; Summers, B.; Leigh, N.; Peters, T.J. Serum carnosinase activities in central nervous system disorders. Clin. Chim. Acta 1994, 225, 57–64. [Google Scholar] [CrossRef]
- Licker, V.; Côte, M.; Lobrinus, J.A.; Rodrigo, N.; Kövari, E.; Hochstrasser, D.F.; Turck, N.; Sanchez, J.C.; Burkhard, P.R. Proteomic profiling of the substantia nigra demonstrates CNDP2 overexpression in Parkinson’s disease. J. Proteomics 2012, 75, 4656–4667. [Google Scholar] [CrossRef]
- Schoen, P.; Everts, H.; de Boer, T.; van Oeveren, W. Serum carnosinase activity in plasma and serum: Validation of a method and values in cardiopulmonary bypass surgery. Clin. Chem. 2003, 49, 1930–1932. [Google Scholar] [CrossRef]
- Holdenrieder, S.; Lütjohann, D.; Geiger, S.; von Bergmann, K.; Stieber, P.; Hamann, G.F. Does brain specific 24S-hydroxycholesterol in plasma indicate the disruption of the blood-brain barrier in patients with ischemic stroke? Neurosci. Lett. 2004, 368, 201–204. [Google Scholar] [CrossRef]
- Kirk, J.; Plumb, J.; Mirakhur, M.; McQuaid, S. Tight junctional abnormality in multiple sclerosis white matter affects all calibres of vessel and is associated with blood-brain barrier leakage and active demyelination. J. Pathol. 2003, 201, 319–327. [Google Scholar] [CrossRef]
- Skoog, I.; Wallin, A.; Fredman, P.; Hesse, C.; Aevarsson, O.; Karlsson, I.; Gottfries, C.G.; Blennow, K. A population study on blood-brain barrier function in 85-year-olds: Relation to Alzheimer’s disease and vascular dementia. Neurology 1998, 50, 966–971. [Google Scholar] [CrossRef]
- Kumar-Singh, S.; Pirici, D.; McGowan, E.; Serneels, S.; Ceuterick, C.; Hardy, J.; Duff, K.; Dickson, D.; van Broeckhoven, C. Dense-core plaques in Tg2576 and PSAPP mouse models of Alzheimer’s disease are centered on vessel walls. Am. J. Pathol. 2005, 167, 527–543. [Google Scholar] [CrossRef]
- Willi, S.M.; Zhang, Y.; Hill, J.B.; Phelan, M.C.; Michaelis, R.C.; Holden, K.R. A deletion in the long arm of chromosome 18 in a child with serum carnosinase deficiency. Pediatr. Res. 1997, 41, 210–213. [Google Scholar]
- Lee, J.H.; Mayeux, R.; Mayo, D.; Mo, J.; Santana, V.; Williamson, J.; Flaquer, A.; Ciappa, A.; Rondon, H.; Estevez, P.; et al. Fine mapping of 10q and 18q for familial Alzheimer’s disease in Caribbean Hispanics. Mol. Psychiatry 2004, 9, 1042–1051. [Google Scholar] [CrossRef]
- Munzar, M.; Levy, S.; Rush, R.; Averback, P. Clinical study of a urinary competitve ELISA for neural thread protein in Alzheimer disease. Neurol. Clin. Neurophysiol. 2002, 2002, 2–8. [Google Scholar]
- Larson, E.B.; Wang, L.; Bowen, J.D.; McCormick, W.C.; Teri, L.; Crane, P.; Kukull, W. Exercise is associated with reduced risk for incident dementia among persons 65 years of age and older. Ann. Intern. Med. 2006, 144, 73–81. [Google Scholar] [CrossRef]
- Vionnet, N.; Tregouet, D.; Kazeem, G.; Gut, I.; Groop, P.H.; Tarnow, L.; Parving, H.H.; Hadjadj, S.; Forsblom, C.; Farrall, M.; et al. Analysis of 14 candidate genes for diabetic nephropathy on chromosome 3q in European populations: Strongest evidence for association with a variant in the promoter region of the adiponectin gene. Diabetes 2006, 55, 3166–3174. [Google Scholar] [CrossRef]
- Freedman, B.I.; Hicks, P.J.; Sale, M.M.; Pierson, E.D.; Langefeld, C.D.; Rich, S.S.; Xu, J.; McDonough, C.; Janssen, B.; Yard, B.A.; et al. A leucine repeat in the carnosinase gene CNDP1 is associated with diabetic end-stage renal disease in European Americans. Nephrol. Dial. Transplant. 2007, 22, 1131–1135. [Google Scholar] [CrossRef]
- Riedl, E.; Koeppel, H.; Brinkkoetter, P.; Sternik, P.; Steinbeisser, H.; Sauerhoefer, S.; Janssen, B.; van der Woude, F.J.; Yard, B.A. A CTG polymorphism in the CNDP1 gene determines the secretion of serum carnosinase in Cos-7-transfected cells. Diabetes 2007, 56, 2410–2413. [Google Scholar] [CrossRef]
- Iyengar, S.K.; Freedman, B.I.; Sedor, J.R. Mining the genome for susceptibility to diabetic nephropathy: The role of large-scale studies and consortia. Semin. Nephrol. 2007, 27, 208–222. [Google Scholar] [CrossRef]
- Wanic, K.; Placha, G.; Dunn, J.; Smiles, A.; Warram, J.H.; Krolewski, A.S. Exclusion of polymorphisms in carnosinase genes (CNDP1 and CNDP2) as a cause of diabetic nephropathy in type 1 diabetes: Results of large case-control and follow-up studies. Diabetes 2008, 57, 2547–2551. [Google Scholar] [CrossRef]
- McDonough, C.W.; Hicks, P.J.; Lu, L.; Langefeld, C.D.; Freedman, B.I.; Bowden, D.W. The influence of carnosinase gene polymorphisms on diabetic nephropathy risk in African-Americans. Hum. Genet. 2009, 126, 265–275. [Google Scholar] [CrossRef]
- Mooyaart, A.L.; Zutinic, A.; Bakker, S.J.L.; Grootendorst, D.C.; Kleefstra, N.; van Valkengoed, I.G.M.; Böhringer, S.; Bilo, H.J.G.; Dekker, F.W.; Bruijn, J.A.; et al. Association between CNDP1 genotype and diabetic nephropathy is sex specific. Diabetes 2010, 59, 1555–1559. [Google Scholar] [CrossRef]
- Riedl, E.; Koeppel, H.; Pfister, F.; Peters, V.; Sauerhoefer, S.; Sternik, P.; Brinkkoetter, P.; Zentgraf, H.; Navis, G.; Henning, R.H.; et al. N-glycosylation of carnosinase influences protein secretion and enzyme activity: Implications for hyperglycemia. Diabetes 2010, 59, 1984–1990. [Google Scholar] [CrossRef]
- Ahluwalia, T.S.; Lindholm, E.; Groop, L.C. Common variants in CNDP1 and CNDP2, and risk of nephropathy in type 2 diabetes. Diabetologia 2011, 54, 2295–2302. [Google Scholar] [CrossRef]
- Chakkera, H.A.; Hanson, R.L.; Kobes, S.; Millis, M.P.; Nelson, R.G.; Knowler, W.C.; Distefano, J.K. Association of variants in the carnosine peptidase 1 gene (CNDP1) with diabetic nephropathy in American Indians. Mol. Genet. Metab. 2011, 103, 185–190. [Google Scholar] [CrossRef]
- Everaert, I.; Mooyaart, A.; Baguet, A.; Zutinic, A.; Baelde, H.; Achten, E.; Taes, Y.; de Heer, E.; Derave, W. Vegetarianism, female gender and increasing age, but not CNDP1 genotype, are associated with reduced muscle carnosine levels in humans. Amino Acids 2011, 40, 1221–1229. [Google Scholar] [CrossRef]
- Peters, V.; Jansen, E.E.W.; Jakobs, C.; Riedl, E.; Janssen, B.; Yard, B.A.; Wedel, J.; Hoffmann, G.F.; Zschocke, J.; Gotthardt, D.; et al. Anserine inhibits carnosine degradation but in human serum carnosinase (CN1) is not correlated with histidine dipeptide concentration. Clin. Chim. Acta 2011, 412, 263–267. [Google Scholar] [CrossRef]
- Peters, V.; Schmitt, C.P.; Zschocke, J.; Gross, M.L.; Brismar, K.; Forsberg, E. Carnosine treatment largely prevents alterations of renal carnosine metabolism in diabetic mice. Amino Acids 2012, 42, 2411–2416. [Google Scholar] [CrossRef]
- McManus, I.R. Enzymic synthesis of anserine in skeletal muscle by N-methylation of carnosine. J. Biol. Chem. 1962, 237, 1207–1211. [Google Scholar]
- Bossy-Wetzel, E.; Schwarzenbacher, R.; Lipton, S.A. Molecular pathways to neurodegeneration. Nat. Med. 2004, 10 (Suppl.), S2–S9. [Google Scholar]
- Blennow, K.; Hampel, H.; Weiner, M.; Zetterberg, H. Cerebrospinal fluid and plasma biomarkers in Alzheimer disease. Nat. Rev. Neurol. 2010, 6, 131–144. [Google Scholar] [CrossRef]
- Hu, Y.; Hosseini, A.; Kauwe, J.S.K.; Gross, J.; Cairns, N.J.; Goate, A.M.; Fagan, A.M.; Townsend, R.R.; Holtzman, D.M. Identification and validation of novel CSF biomarkers for early stages of Alzheimer’s disease. Proteomics Clin. Appl. 2007, 1, 1373–1384. [Google Scholar] [CrossRef]
- Perrin, R.J.; Craig-Schapiro, R.; Malone, J.P.; Shah, A.R.; Gilmore, P.; Davis, A.E.; Roe, C.M.; Peskind, E.R.; Li, G.; Galasko, D.R.; et al. Identification and Validation of Novel Cerebrospinal Fluid Biomarkers for Staging Early Alzheimer’s Disease. PLoS One 2011, 6, e16032. [Google Scholar] [CrossRef]
- Renner, C.; Zemitzsch, N.; Fuchs, B.; Geiger, K.D.; Hermes, M.; Hengstler, J.; Gebhardt, R.; Meixensberger, J.; Gaunitz, F. Carnosine retards tumor growth in vivo in an NIH3T3-HER2/neu mouse model. Mol. Cancer 2010, 9, 2. [Google Scholar] [CrossRef]
- Pahari, D.R.; Gu, Y.J.; van Oeveren, W.; El-Essawi, A.; Harringer, W.; Brouwer, R.M. Effect of minimized perfusion circuit on brain injury markers carnosinase and brain-type fatty binding protein in coronary artery bypass grafting patients. Artif. Organs 2013, 37, 128–135. [Google Scholar] [CrossRef]
- Sale, C.; Artioli, G.G.; Gualano, B.; Saunders, B.; Hobson, R.M.; Harris, R.C. Carnosine: From exercise performance to health. Amino Acids 2013, 44, 1477–1491. [Google Scholar] [CrossRef]
- Derave, W.; Everaert, I.; Beeckman, S.; Baguet, A. Muscle carnosine metabolism and beta-alanine supplementation in relation to exercise and training. Sports Med. 2010, 40, 247–263. [Google Scholar] [CrossRef]
- Artioli, G.G.; Gualano, B.; Smith, A.; Stout, J.; Lancha, A.H., Jr. Role of beta-alanine supplementation on muscle carnosine and exercise performance. Med. Sci. Sports Exercise 2010, 42, 1162–1173. [Google Scholar]
- Bellia, F.; Vecchio, G.; Rizzarelli, E. Carnosine derivatives: New multifunctional drug-like molecules. Amino Acids 2012, 43, 153–163. [Google Scholar] [CrossRef]
- Grasso, G.I.; Bellia, F.; Arena, G.; Vecchio, G.; Rizzarelli, E. Noncovalent interaction-driven stereoselectivity of copper(II) complexes with cyclodextrin derivatives of L- and D-carnosine. Inorg. Chem. 2011, 50, 4917–4924. [Google Scholar] [CrossRef]
- Grasso, G.I.; Arena, G.; Bellia, F.; Maccarrone, G.; Parrinello, M.; Pietropaolo, A.; Vecchio, G.; Rizzarelli, E. Intramolecular weak interactions in the thermodynamic stereoselectivity of copper(II) complexes with carnosine-trehalose conjugates. Chem. Eur. J. 2011, 17, 9448–9455. [Google Scholar] [CrossRef]
- Lanza, V.; Bellia, F.; D’Agata, R.; Grasso, G.; Rizzarelli, E.; Vecchio, G. New glycoside derivatives of carnosine and analogs resistant to carnosinase hydrolysis: Synthesis and characterization of their copper(II) complexes. J. Inorg. Biochem. 2011, 105, 181–188. [Google Scholar] [CrossRef]
- Stvolinsky, S.L.; Bulygina, E.R.; Fedorova, T.N.; Meguro, K.; Sato, T.; Tyulina, O.V.; Abe, H.; Boldyrev, A.A. Biological activity of novel synthetic derivatives of carnosine. Cell. Mol. Neurobiol. 2010, 30, 395–404. [Google Scholar] [CrossRef]
- Astete, C.E.; Songe Meador, D.; Spivak, D.; Sabliov, C. Synthesis of vitamin E-carnosine (VECAR): New antioxidant molecule with potential application in atherosclerosis. Synth. Commun. 2013, 43, 1299–1313. [Google Scholar] [CrossRef]
- Sozio, P.; Iannitelli, A.; Cerasa, L.S.; Cacciatore, I.; Cornacchia, C.; Giorgioni, G.; Ricciutelli, M.; Nasuti, C.; Cantalamessa, F.; di Stefano, A. New L-dopa codrugs as potential antiparkinson agents. Arch. Pharm. 2008, 341, 412–417. [Google Scholar] [CrossRef]
- Nativi, C.; Gualdani, R.; Dragoni, E.; di Cesare Mannelli, L.; Sostegni, S.; Norcini, M.; Gabrielli, G.; la Marca, G.; Richichi, B.; Francesconi, O.; et al. A TRPA1 antagonist reverts oxaliplatin-induced neuropathic pain. Sci. Rep. 2013, 3. [Google Scholar] [CrossRef]
- Saada, M.C.; Montero, J.L.; Vullo, D.; Scozzafava, A.; Winum, J.Y.; Supuran, C.T. Carbonic anhydrase activators: Gold nanoparticles coated with derivatized histamine, histidine, and carnosine show enhanced activatory effects on several mammalian isoforms. J. Med. Chem. 2011, 54, 1170–1177. [Google Scholar]
- Bellia, F.; Oliveri, V.; Rizzarelli, E.; Vecchio, G. New derivative of carnosine for nanoparticle assemblies. Eur. J. Med. Chem. 2013, 70, 225–232. [Google Scholar] [CrossRef]
- Bertinaria, M.; Rolando, B.; Giorgis, M.; Montanaro, G.; Guglielmo, S.; Buonsanti, M.F.; Carabelli, V.; Gavello, D.; Daniele, P.G.; Fruttero, R.; et al. Synthesis, physicochemical characterization, and biological activities of new carnosine derivatives stable in human serum as potential neuroprotective agents. J. Med. Chem. 2011, 54, 611–621. [Google Scholar] [CrossRef]
- Supuran, C.T.; Briganti, F.; Tilli, S.; Chegwidden, W.R.; Scozzafava, A. Carbonic anhydrase inhibitors: Sulfonamides as antitumor agents? Biorg. Med. Chem. 2001, 9, 703–714. [Google Scholar] [CrossRef]
- Nielsen, C.U.; Supuran, C.T.; Scozzafava, A.; Frokjaer, S.; Steffansen, B.; Brodin, B. Transport characteristics of L-carnosine and the anticancer derivative 4-toluenesulfonylureido-carnosine in a human epithelial cell line. Pharm. Res. 2002, 19, 1337–1344. [Google Scholar] [CrossRef]
- Vistoli, G.; Orioli, M.; Pedretti, A.; Regazzoni, L.; Canevotti, R.; Negrisoli, G.; Carini, M.; Aldini, G. Design, synthesis, and evaluation of carnosine derivatives as selective and efficient sequestering agents of cytotoxic reactive carbonyl species. ChemMedChem 2009, 4, 967–975. [Google Scholar]
- Grasso, G.I.; Arena, G.; Bellia, F.; Rizzarelli, E.; Vecchio, G. Copper(II)-chelating homocarnosine glycoconjugate as a new multifunctional compound. J. Inorg. Biochem. 2014, 131, 56–63. [Google Scholar]
- Orioli, M.; Vistoli, G.; Regazzoni, L.; Pedretti, A.; Lapolla, A.; Rossoni, G.; Canevotti, R.; Gamberoni, L.; Previtali, M.; Carini, M.; et al. Design, synthesis, ADME properties, and pharmacological activities of beta-alanyl-d-histidine (d-carnosine) prodrugs with improved bioavailability. ChemMedChem 2011, 6, 1269–1282. [Google Scholar] [CrossRef]
- Amorini, A.M.; Bellia, F.; di Pietro, V.; Giardina, B.; la Mendola, D.; Lazzarino, G.; Sortino, S.; Tavazzi, B.; Rizzarelli, E.; Vecchio, G. Synthesis and antioxidant activity of new homocarnosine β-cyclodextrin conjugates. Eur. J. Med. Chem. 2007, 42, 910–920. [Google Scholar] [CrossRef]
- Bellia, F.; Amorini, A.M.; la Mendola, D.; Vecchio, G.; Tavazzi, B.; Giardina, B.; di Pietro, V.; Lazzarino, G.; Rizzarelli, E. New glycosidic derivatives of histidine-containing dipeptides with antioxidant properties and resistant to carnosinase activity. Eur. J. Med. Chem. 2008, 43, 373–380. [Google Scholar] [CrossRef]
- Bellia, F.; la Mendola, D.; Maccarrone, G.; Mineo, P.; Vitalini, D.; Scamporrino, E.; Sortino, S.; Vecchio, G.; Rizzarelli, E. Copper(II) complexes with β-cyclodextrin-homocarnosine conjugates and their antioxidant activity. Inorg. Chim. Acta 2007, 360, 945–954. [Google Scholar] [CrossRef]
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