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Molecules 2014, 19(2), 1635-1650; doi:10.3390/molecules19021635
Article

Simultaneous Determination of Fifteen Constituents of Jitai Tablet Using Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Electrospray Tandem Mass Spectrometry

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Received: 30 November 2013; in revised form: 24 January 2014 / Accepted: 24 January 2014 / Published: 28 January 2014
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Abstract: An ultra-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was developed for simultaneous determination of fifteen constituents in Jitai tablet (JTT), a complex Traditional Chinese Medicine prescription (TCMP) used in treating opiate addiction. Benefitting from a small particle size (1.8 µm) C18 column, accelerated analysis with satisfactory resolution, sensitivity and selectivity were achieved in a single run within 7 min with linear gradient elution of acetonitrile-0.1% (v/v) formic acid in water. The analytical signal was obtained by multiple reaction monitoring transitions via electrospray ionization source operating in both positive and negative ionization mode. The approach was validated for linearity, sensitivity, precision, repeatability, stability and recovery. All analytes showed good linearity over a wide concentration range (r > 0.99). The method limits ranged from 0.03 ng/mL to 19.35 ng/mL which are sensitive enough for quality control studies. The developed method was successfully applied to the simultaneous determination of fifteen constituents in JTT. In conclusion, our experimental results demonstrate that UHPLC-ESI-MS/MS is a useful approach for the overall quality assessment of complex TCMPs.
Keywords: Jitai tablet; UHPLC-ESI-MS/MS; quality evaluation; TCMP Jitai tablet; UHPLC-ESI-MS/MS; quality evaluation; TCMP
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, S.; Fu, P.; Liu, L.; Wang, L.; Peng, C.; Zhang, W.; Liu, R. Simultaneous Determination of Fifteen Constituents of Jitai Tablet Using Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Electrospray Tandem Mass Spectrometry. Molecules 2014, 19, 1635-1650.

AMA Style

Wang S, Fu P, Liu L, Wang L, Peng C, Zhang W, Liu R. Simultaneous Determination of Fifteen Constituents of Jitai Tablet Using Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Electrospray Tandem Mass Spectrometry. Molecules. 2014; 19(2):1635-1650.

Chicago/Turabian Style

Wang, Shuping; Fu, Peng; Liu, Lei; Wang, Lingling; Peng, Chengcheng; Zhang, Weidong; Liu, Runhui. 2014. "Simultaneous Determination of Fifteen Constituents of Jitai Tablet Using Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Electrospray Tandem Mass Spectrometry." Molecules 19, no. 2: 1635-1650.


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