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Molecules 2014, 19(11), 19078-19096; doi:10.3390/molecules191119078

Composition and Antioxidant Activity of the Anthocyanins of the Fruit of Berberis heteropoda Schrenk

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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Received: 16 October 2014 / Revised: 28 October 2014 / Accepted: 3 November 2014 / Published: 19 November 2014
(This article belongs to the Special Issue Anthocyanins)
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Abstract

In present study, the anthocyanin composition and content of the fruit of B. heteropoda Schrenk were determined for the first time. The total anthocyanins were extracted from the fruit of B. heteropoda Schrenk using 0.5% HCl in 80% methanol and were then purified using an AB-8 macroporous resin column. The purified anthocyanin extract (PAE) was evaluated by high-performance liquid chromatography with a diode array detector (HPLC-DAD) and HPLC-high resolution-electrospray ionization-mass spectrometry (HPLC-HR-ESI-MS) under the same experimental conditions. The results revealed the presence of seven different anthocyanins. The major anthocyanins purified by preparative HPLC were confirmed to be delphinidin-3-O-glucopyranoside (30.3%), cyanidin-3-O-glucopyranoside (33.5%), petunidin-3-Ο-glucopyranoside (10.5%), peonidin-3-O-glucopyranoside (8.5%) and malvidin-3-O-glucopyranoside (13.8%) using HPLC-HR-ESI-MS and NMR spectroscopy. The total anthocyanin content was 2036.6 ± 2.2 mg/100 g of the fresh weight of B. heteropoda Schrenk fruit. In terms of its total reducing capacity assay, DPPH radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay and ABTS radical cation-scavenging activity assay, the PAE also showed potent antioxidant activity. The results are valuable for illuminating anthocyanins composition of B. heteropoda Schrenk and for further utilising them as a promising anthocyanin pigment source. This research enriched the chemical information of B. heteropoda Schrenk. View Full-Text
Keywords: Berberis heteropoda Schrenk; HPLC-DAD; HPLC-HR-ESI-MS; TAC; DPPH; FRAP; ABTS Berberis heteropoda Schrenk; HPLC-DAD; HPLC-HR-ESI-MS; TAC; DPPH; FRAP; ABTS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sun, L.-L.; Gao, W.; Zhang, M.-M.; Li, C.; Wang, A.-G.; Su, Y.-L.; Ji, T.-F. Composition and Antioxidant Activity of the Anthocyanins of the Fruit of Berberis heteropoda Schrenk. Molecules 2014, 19, 19078-19096.

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