Next Article in Journal
Femtosecond Laser Spectroscopy of the Rhodopsin Photochromic Reaction: A Concept for Ultrafast Optical Molecular Switch Creation (Ultrafast Reversible Photoreaction of Rhodopsin)
Previous Article in Journal
In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(11), 18332-18350; doi:10.3390/molecules191118332

Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba

1
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan
2
Department of Nursing, Hsin Sheng College of Medical Care and Management, Taoyuan 32544, Taiwan
3
Department of Nursing, Hungkuang University, Taichung 43302, Taiwan
4
Chinese Crude Drug Pharmacy, China Medical University Hospital, Taichung 40402, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 24 September 2014 / Revised: 3 November 2014 / Accepted: 6 November 2014 / Published: 10 November 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [568 KB, uploaded 10 November 2014]   |  

Abstract

This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities. View Full-Text
Keywords: Andrographis Herba; quantitative determination; chromatographic fingerprint; antioxidant activities; correlation analysis Andrographis Herba; quantitative determination; chromatographic fingerprint; antioxidant activities; correlation analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhao, Y.; Kao, C.-P.; Wu, K.-C.; Liao, C.-R.; Ho, Y.-L.; Chang, Y.-S. Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba. Molecules 2014, 19, 18332-18350.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top