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Molecules 2014, 19(10), 16373-16380; doi:10.3390/molecules191016373

The Effect of Freeze/Thaw Cycles on Reproducibility of Metabolic Profiling of Marine Microalgal Extracts Using Direct Infusion High-Resolution Mass Spectrometry (HR-MS)

1
Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, Tromsø N-9037, Norway
2
MabCent-SFI, UiT The Arctic University of Norway, Tromsø N-9037, Norway
3
Marbio, UiT The Arctic University of Norway, Tromsø N-9037, Norway
*
Author to whom correspondence should be addressed.
Received: 23 July 2014 / Revised: 3 October 2014 / Accepted: 9 October 2014 / Published: 13 October 2014
(This article belongs to the Section Metabolites)
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Abstract

During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at −78 °C (one freeze/thaw cycle), and after additional seven days at −20 °C (two freeze/thaw cycles). Repeated direct infusion high-resolution mass spectrometry analysis of microalgae extracts of the same sample showed that reproducibility was ca. 90% when a fresh (unfrozen) sample was analyzed. The overall reproducibility decreased further by ca. 10% after the first freeze/thaw-cycle, and after one more freeze/thaw cycle the reproducibility decreased further by ca. 7%. The decrease in reproducibility after freeze-thaw cycles could be attributed to sample degradation and not to instrument variability. View Full-Text
Keywords: extracts; metabolic profiling; mass spectrometry; freeze/thaw cycles; stability extracts; metabolic profiling; mass spectrometry; freeze/thaw cycles; stability
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Eilertsen, H.C.; Huseby, S.; Degerlund, M.; Eriksen, G.K.; Ingebrigtsen, R.A.; Hansen, E. The Effect of Freeze/Thaw Cycles on Reproducibility of Metabolic Profiling of Marine Microalgal Extracts Using Direct Infusion High-Resolution Mass Spectrometry (HR-MS). Molecules 2014, 19, 16373-16380.

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