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Molecules 2014, 19(1), 1328-1343; doi:10.3390/molecules19011328
Article

EGF Receptor-Dependent Mechanism May be Involved in the Tamm–Horsfall Glycoprotein-Enhanced PMN Phagocytosis via Activating Rho Family and MAPK Signaling Pathway

1,†
, 2,†
, 3
, 3
, 4
, 5
, 6
 and 2,6,*
Received: 3 December 2013; in revised form: 13 January 2014 / Accepted: 16 January 2014 / Published: 21 January 2014
(This article belongs to the Special Issue Oligosaccharides and Glyco-Conjugates)
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Abstract: Our previous studies showed that urinary Tamm–Horsfall glycoprotein (THP) potently enhanced polymorphonuclear neutrophil (PMN) phagocytosis. However, the domain structure(s), signaling pathway and the intracellular events responsible for THP-enhanced PMN phagocytosis remain to be elucidated. THP was purified from normal human urine. The human promyelocytic leukemia cell line HL-60 was induced to differentiate into PMNs by all-trans retinoid acid. Pretreatment with different MAPK and PI3K inhibitors was used to delineate signaling pathways in THP-enhanced PMN phagocytosis. Phosphorylation of molecules responsible for PMN phagocytosis induced by bacterial lipopolysaccharide (LPS), THP, or human recombinant epidermal growth factor (EGF) was evaluated by western blot. A p38 MAPK inhibitor, SB203580, effectively inhibited both spontaneous and LPS- and THP-induced PMN phagocytosis. Both THP and LPS enhanced the expression of the Rho family proteins Cdc42 and Rac that may lead to F-actin re-arrangement. Further studies suggested that THP and EGF enhance PMN and differentiated HL-60 cell phagocytosis in a similar pattern. Furthermore, the EGF receptor inhibitor GW2974 significantly suppressed THP- and EGF-enhanced PMN phagocytosis and p38 and ERK1/2 phosphorylation in differentiated HL-60 cells. We conclude that EGF receptor-dependent signaling may be involved in THP-enhanced PMN phagocytosis by activating Rho family and MAP kinase.
Keywords: Tamm-Horsfall glycoprotein; phagocytosis; EGF-like domains; Rho family; MAP kinase Tamm-Horsfall glycoprotein; phagocytosis; EGF-like domains; Rho family; MAP kinase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, K.-J.; Siao, S.-C.; Wu, C.-H.; Shen, C.-Y.; Wu, T.-H.; Tsai, C.-Y.; Hsieh, S.-C.; Yu, C.-L. EGF Receptor-Dependent Mechanism May be Involved in the Tamm–Horsfall Glycoprotein-Enhanced PMN Phagocytosis via Activating Rho Family and MAPK Signaling Pathway. Molecules 2014, 19, 1328-1343.

AMA Style

Li K-J, Siao S-C, Wu C-H, Shen C-Y, Wu T-H, Tsai C-Y, Hsieh S-C, Yu C-L. EGF Receptor-Dependent Mechanism May be Involved in the Tamm–Horsfall Glycoprotein-Enhanced PMN Phagocytosis via Activating Rho Family and MAPK Signaling Pathway. Molecules. 2014; 19(1):1328-1343.

Chicago/Turabian Style

Li, Ko-Jen; Siao, Sue-Cien; Wu, Cheng-Han; Shen, Chieh-Yu; Wu, Tsai-Hung; Tsai, Chang-Youh; Hsieh, Song-Chou; Yu, Chia-Li. 2014. "EGF Receptor-Dependent Mechanism May be Involved in the Tamm–Horsfall Glycoprotein-Enhanced PMN Phagocytosis via Activating Rho Family and MAPK Signaling Pathway." Molecules 19, no. 1: 1328-1343.



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