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Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
Faculdade de Farmácia, Universidade Federal da Bahia, Rua Barão de Jeremoabo, s/n, 41810-290, Salvador (BA), Brazil
Programa de Pós- graduação em Biotecnologia, Universidade Estadual de Feira de Santana, 44031-460, Feira de Santana (BA), Brazil
Instituto de Química, Universidade Federal da Bahia, Campus de Ondina, 40170290, Salvador (BA), Brazil
Empresa Baiana de Desenvolvimento Agrícola S.A, 40170-110, Salvador (BA), Brazil
Centro de Ciências da Saúde, Universidade Federal de Recôncavo Baiano, 44574-490, Santo Antonio de Jesus (BA), Brazil
* Author to whom correspondence should be addressed.
Received: 22 July 2013; in revised form: 10 August 2013 / Accepted: 22 August 2013 / Published: 26 August 2013
Abstract: Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, β-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and β-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments.
Keywords: Plectranthus ornatus; Lamiaceae; in vitro cultivation; HCA and PCA; HS-SPME; volatile compounds
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Passinho-Soares, H.C.; Meira, P.R.; David, J.P.; Mesquita, P.R.R.; Vale, A.E.; de M. Rodrigues, F.; de P. Pereira, P.A.; de Santana, J.R.F.; de Oliveira, F.S.; de Andrade, J.B.; David, J.M. Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae). Molecules 2013, 18, 10320-10333.
Passinho-Soares HC, Meira PR, David JP, Mesquita PRR, Vale AE, de M. Rodrigues F, de P. Pereira PA, de Santana JRF, de Oliveira FS, de Andrade JB, David JM. Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae). Molecules. 2013; 18(9):10320-10333.
Passinho-Soares, Helna C.; Meira, Paloma R.; David, Juceni P.; Mesquita, Paulo R.R.; Vale, Ademir E.; de M. Rodrigues, Frederico; de P. Pereira, Pedro A.; de Santana, José R.F.; de Oliveira, Fabio S.; de Andrade, Jailson B.; David, Jorge M. 2013. "Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)." Molecules 18, no. 9: 10320-10333.