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Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1
Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico
Doctorado en Desarrollo de Productos Bióticos, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P. O. Box 24, Yautepec 62730, Morelos, Mexico
* Author to whom correspondence should be addressed.
Received: 22 April 2013; in revised form: 29 May 2013 / Accepted: 6 June 2013 / Published: 10 July 2013
Abstract: Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants’ leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25R)-5α-spirostan-3β,6α,23α-triol-3,6-di-O-β-D-glucopyranoside (cantalasaponin-1) through 1H- and 13C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.
Keywords: Agavaceae; A. angustifolia Haw; A. tequilana Weber; A. americana; cantalasaponin-1; inflammation
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Monterrosas-Brisson, N.; Ocampo, M.L.A.; Jiménez-Ferrer, E.; Jiménez-Aparicio, A.R.; Zamilpa, A.; Gonzalez-Cortazar, M.; Tortoriello, J.; Herrera-Ruiz, M. Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1. Molecules 2013, 18, 8136-8146.
Monterrosas-Brisson N, Ocampo MLA, Jiménez-Ferrer E, Jiménez-Aparicio AR, Zamilpa A, Gonzalez-Cortazar M, Tortoriello J, Herrera-Ruiz M. Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1. Molecules. 2013; 18(7):8136-8146.
Monterrosas-Brisson, Nayeli; Ocampo, Martha L.A.; Jiménez-Ferrer, Enrique; Jiménez-Aparicio, Antonio R.; Zamilpa, Alejandro; Gonzalez-Cortazar, Manases; Tortoriello, Jaime; Herrera-Ruiz, Maribel. 2013. "Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1." Molecules 18, no. 7: 8136-8146.