Protective Mechanisms of Guanosine from Solanum lycopersicum on Agonist-Induced Platelet Activation: Role of sCD40L
Abstract
:1. Introduction
2. Results and Discussion
2.1. Bioassay-Guided Isolation of Antiplatelet Compound
2.2. Identification of the Antiplatelet Compound
2.3. Effects of Guanosine on Platelet Function
2.4. Inhibitory Effect of Guanosine on Platelet Spreading on Immobilized Collagen
2.5. Guanosine Reduces Platelet Adhesion and Aggregation under Flow Conditions
2.6. Inhibitory Effect of Guanosine on Levels of sCD40L
3. Experimental
3.1. Chemicals and Reagents
3.2. Processing Material
3.3. Bioassay-Guided Isolation of Bioactive Compound
3.4. Chemical Identification of Antiplatelet Compound
3.4.1. Spectral Scanning
3.4.2. NMR Analysis
3.4.3. HPLC Analysis
3.5. Preparation of Human Platelet Suspensions
3.6. Measurement of Platelet Secretion
3.7. Platelet Spreading Assay
3.8. Measurement of Platelet Aggregation
3.9. Analysis of Platelet Adhesion and Aggregation under Controlled Flow
3.10. Measurement of sCD40L Levels
3.11. Statistical Analysis
4. Conclusion
Acknowledgments
Conflicts of Interest
References
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Fuentes, E.; Alarcón, M.; Astudillo, L.; Valenzuela, C.; Gutiérrez, M.; Palomo, I. Protective Mechanisms of Guanosine from Solanum lycopersicum on Agonist-Induced Platelet Activation: Role of sCD40L. Molecules 2013, 18, 8120-8135. https://doi.org/10.3390/molecules18078120
Fuentes E, Alarcón M, Astudillo L, Valenzuela C, Gutiérrez M, Palomo I. Protective Mechanisms of Guanosine from Solanum lycopersicum on Agonist-Induced Platelet Activation: Role of sCD40L. Molecules. 2013; 18(7):8120-8135. https://doi.org/10.3390/molecules18078120
Chicago/Turabian StyleFuentes, Eduardo, Marcelo Alarcón, Luis Astudillo, Claudio Valenzuela, Margarita Gutiérrez, and Iván Palomo. 2013. "Protective Mechanisms of Guanosine from Solanum lycopersicum on Agonist-Induced Platelet Activation: Role of sCD40L" Molecules 18, no. 7: 8120-8135. https://doi.org/10.3390/molecules18078120