Molecules 2013, 18(7), 7584-7599; doi:10.3390/molecules18077584
Article

Anxiolytic-Like and Antinociceptive Effects of 2(S)-Neoponcirin in Mice

1 Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Mexico D.F. C.P. 04960, Mexico 2 Laboratorio de Fitofarmacología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada Mexico-Xochimilco 101, Col. San Lorenzo Huipulco, Delegación Tlalpan, Mexico D.F, 14370, Mexico 3 Instituto de Química, Universidad Nacional Autónoma de Mexico, Coyoacan, Mexico D.F. 04510, Mexico
* Author to whom correspondence should be addressed.
Received: 9 May 2013; in revised form: 12 June 2013 / Accepted: 19 June 2013 / Published: 28 June 2013
PDF Full-text Download PDF Full-Text [300 KB, uploaded 28 June 2013 11:44 CEST]
Abstract: Study aims: 2(S)-neopincirin (NEO) is a constituent from of Clinopodium mexicanum, which is used in traditional Mexican herbal medicine for its tranquilizing and analgesic properties. This study investigated the anxiolytic-like, sedative and antinociceptive effects of NEO in several mice models. Material and methods: The anxiolytic-like effect was evaluated in the hole-board (HBT) and Open Field Tests (OFT); sedative effect was evaluated in sleeping time induced by sodium pentobarbital, and its antinociceptive actions were measured in the hot plate test. To evaluate if the GABA receptor could be involved in the anxiolytic-like effect produced by NEO, in independent experiments, the effects produced by co-administration of NEO plus muscimol (MUS) and NEO plus Pitrotoxin (PTX) were evaluated in the HBT. Results: NEO was isolated from Clinopodium mexicanum leaves. The NMR, MS and optic rotation data helped establish its identity as (2S)-5-hydroxy-4′-methoxyflavanone-7-O-{β-glucopyranosyl-(1→6)-β-rhamnoside}. NEO showed an anxiolytic-like effect and was able to counter the nociception induced by a thermal stimulus in a dose-dependent manner. PTX blocked the anxiolytic-like effect of NEO, while MUS was able to enhance it. Conclusions: The findings of present work demonstrated that NEO possesses anxiolytic-like and antinociceptive effects in mice. Such effects are not associated with changes in the locomotor activity. These results supported the notion that anxiolytic-like effect of NEO involves the participation of GABAergic system.
Keywords: neoponcirin; anxiolytic-like; Clinopodium mexicanum; toronjil de monte; analgesic; GABAergic system

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Cassani, J.; Araujo, A.G.E.; Martínez-Vázquez, M.; Manjarrez, N.; Moreno, J.; Estrada-Reyes, R. Anxiolytic-Like and Antinociceptive Effects of 2(S)-Neoponcirin in Mice. Molecules 2013, 18, 7584-7599.

AMA Style

Cassani J, Araujo AGE, Martínez-Vázquez M, Manjarrez N, Moreno J, Estrada-Reyes R. Anxiolytic-Like and Antinociceptive Effects of 2(S)-Neoponcirin in Mice. Molecules. 2013; 18(7):7584-7599.

Chicago/Turabian Style

Cassani, Julia; Araujo, Anna G.E.; Martínez-Vázquez, Mariano; Manjarrez, Norberto; Moreno, Julia; Estrada-Reyes, Rosa. 2013. "Anxiolytic-Like and Antinociceptive Effects of 2(S)-Neoponcirin in Mice." Molecules 18, no. 7: 7584-7599.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert