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Molecules 2013, 18(5), 5072-5090; doi:10.3390/molecules18055072
Article

Characterisation of Phenolic Compounds in South African Plum Fruits (Prunus salicina Lindl.) using HPLC Coupled with Diode-Array, Fluorescence, Mass Spectrometry and On-Line Antioxidant Detection

1
, 1,2
 and 2,*
Received: 7 April 2013; in revised form: 24 April 2013 / Accepted: 25 April 2013 / Published: 2 May 2013
(This article belongs to the Special Issue Phytochemicals: Analytical and Medicinal Chemistry)
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Abstract: Phenolic compounds are abundant secondary metabolites in plums, with potential health benefits believed to be due to their antioxidant activity, amongst others. Phenolic characterisation of South African Prunus salicina Lindl. plums is necessary to fully evaluate their potential health benefits. An HPLC method using diode-array detection (DAD) for quantification of phenolic compounds was improved and fluorescence detection (FLD) was added for quantification of flavan-3-ols. Validation of the HPLC-DAD-FLD method showed its suitability for quantification of 18 phenolic compounds, including flavan-3-ols using FLD, and phenolic acids, anthocyanins and flavonols using DAD. The method was suitable for characterisation of the phenolic composition of 11 South African plum cultivars and selections, including various types with yellow and red skin and flesh. The method was used in conjunction with mass spectrometry (MS) to identify 24 phenolic compounds. Neochlorogenic acid and cyanidin-3-O-glucoside were the major compounds in most of the plums, while cyanidin-3-O-glucoside was absent in Sun Breeze plums with yellow skin and flesh. Post-column on-line coupling of the ABTS•+ scavenging assay with HPLC-DAD enabled qualitative evaluation of the relative contribution of individual phenolic compounds to the antioxidant activity. The flavan-3-ols, neochlorogenic acid and cyanidin-3-O-glucoside displayed the largest antioxidant response peaks.
Keywords: diode-array detection; fluorescence detection; HPLC; mass spectrometry; method validation; on-line antioxidant activity; phenolic compound; plum fruit; Prunus salicina Lindl. diode-array detection; fluorescence detection; HPLC; mass spectrometry; method validation; on-line antioxidant activity; phenolic compound; plum fruit; Prunus salicina Lindl.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Venter, A.; Joubert, E.; de Beer, D. Characterisation of Phenolic Compounds in South African Plum Fruits (Prunus salicina Lindl.) using HPLC Coupled with Diode-Array, Fluorescence, Mass Spectrometry and On-Line Antioxidant Detection. Molecules 2013, 18, 5072-5090.

AMA Style

Venter A, Joubert E, de Beer D. Characterisation of Phenolic Compounds in South African Plum Fruits (Prunus salicina Lindl.) using HPLC Coupled with Diode-Array, Fluorescence, Mass Spectrometry and On-Line Antioxidant Detection. Molecules. 2013; 18(5):5072-5090.

Chicago/Turabian Style

Venter, Alet; Joubert, Elizabeth; de Beer, Dalene. 2013. "Characterisation of Phenolic Compounds in South African Plum Fruits (Prunus salicina Lindl.) using HPLC Coupled with Diode-Array, Fluorescence, Mass Spectrometry and On-Line Antioxidant Detection." Molecules 18, no. 5: 5072-5090.


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