Molecules 2013, 18(4), 3859-3871; doi:10.3390/molecules18043859
Article

HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex paraguariensis Beverages and On-Line Evaluation of Individual Antioxidant Activity

1, 2, 3 and 1,* email
Received: 27 December 2012; in revised form: 19 March 2013 / Accepted: 20 March 2013 / Published: 28 March 2013
(This article belongs to the Special Issue Methods in Polyphenol Analysis)
Download PDF [318 KB, uploaded 18 June 2014]
Abstract: “Chimarrão” and “tererê” are maté (dried, toasted and milled Ilex paraguariensis leaves and stemlets) beverages widely consumed in South America. This paper describes the application of HPLC-DAD-ESI/MS method for the identification and quantification of caffeoylquinic acids (CQA), flavonol glycosides and purine alkaloids in these beverages. The beverage samples were prepared from commercial lots of maté from Southern Brazil. The caffeoylquinic acids, 4,5-diCQA, 3-CQA, 5-CQA, and 4-CQA were the major compounds, having 238–289, 153–242, 183–263, and 123–188 μg/mL, respectively, for chimarrão and 206–265, 122–218, 164–209, 103–169 μg/mL, respectively, for tererê. Caffeine also had high amounts while glycosides of quercetin and kaempferol were found at much lower levels. The individual antioxidant activity was also determined by an on-line system that measured their ABTS•+ radical scavenging activity, showing that the antioxidant capacity was not proportional to the concentrations of the phenolic compounds. 3-CQA, quercetina-3-O-ramnosylglucoside, and quercetina-3-O-glucoside were the major contributors to the antioxidant capacity, although the quercetin glycosides had concentrations less than 10 times that of 3-CQA.
Keywords: beverage; Ilex paraguarienses; phenolic compounds; HPLC-DAD-ESI-MS; antioxidant capacity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Peres, R.G.; Tonin, F.G.; Tavares, M.F.M.; Rodriguez-Amaya, D.B. HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex paraguariensis Beverages and On-Line Evaluation of Individual Antioxidant Activity. Molecules 2013, 18, 3859-3871.

AMA Style

Peres RG, Tonin FG, Tavares MFM, Rodriguez-Amaya DB. HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex paraguariensis Beverages and On-Line Evaluation of Individual Antioxidant Activity. Molecules. 2013; 18(4):3859-3871.

Chicago/Turabian Style

Peres, Renato G.; Tonin, Fernando G.; Tavares, Marina F.M.; Rodriguez-Amaya, Delia B. 2013. "HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex paraguariensis Beverages and On-Line Evaluation of Individual Antioxidant Activity." Molecules 18, no. 4: 3859-3871.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert