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Molecules 2013, 18(2), 2222-2235; doi:10.3390/molecules18022222
Article

Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products

1,2,3,4,†
,
1,2,3,4,†
,
1,2,3,4,* , 1,2,3,* , 1,2,3,5
 and
1,2,5
1 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China 2 Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China 3 Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China 4 Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China 5 Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 13 December 2012 / Revised: 24 January 2013 / Accepted: 28 January 2013 / Published: 11 February 2013
Download PDF [451 KB, 18 June 2014; original version 18 June 2014]

Abstract

This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg−1, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg−1, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg−1.
Keywords: aflatoxins; immunoaffinity column; amino-silica gel; microparticle conjugate; agri-products; HPLC aflatoxins; immunoaffinity column; amino-silica gel; microparticle conjugate; agri-products; HPLC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ma, F.; Chen, R.; Li, P.; Zhang, Q.; Zhang, W.; Hu, X. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules 2013, 18, 2222-2235.

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