Molecules 2013, 18(2), 2222-2235; doi:10.3390/molecules18022222
Article

Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products

Fei Ma 1,2,3,4,† email, Ran Chen 1,2,3,4,† email, Peiwu Li 1,2,3,4,* email, Qi Zhang 1,2,3,* email, Wen Zhang 1,2,3,5 email and Xiaofeng Hu 1,2,5 email
1 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China 2 Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China 3 Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China 4 Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China 5 Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China † These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 13 December 2012; in revised form: 24 January 2013 / Accepted: 28 January 2013 / Published: 11 February 2013
PDF Full-text Download PDF Full-Text [451 KB, Updated Version, uploaded 18 February 2013 12:23 CET]
The original version is still available [452 KB, uploaded 11 February 2013 09:23 CET]
Abstract: This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg−1, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg−1, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg−1.
Keywords: aflatoxins; immunoaffinity column; amino-silica gel; microparticle conjugate; agri-products; HPLC

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Ma, F.; Chen, R.; Li, P.; Zhang, Q.; Zhang, W.; Hu, X. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules 2013, 18, 2222-2235.

AMA Style

Ma F, Chen R, Li P, Zhang Q, Zhang W, Hu X. Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products. Molecules. 2013; 18(2):2222-2235.

Chicago/Turabian Style

Ma, Fei; Chen, Ran; Li, Peiwu; Zhang, Qi; Zhang, Wen; Hu, Xiaofeng. 2013. "Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products." Molecules 18, no. 2: 2222-2235.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert