In Vitro and In Vivo Antiangiogenic Activity of Caged Polyprenylated Xanthones Isolated from Garcinia hanburyi Hook. f.
Abstract
:1. Introduction
2. Results and Discussion
Xanthone | IC50 (μM) | ||||
---|---|---|---|---|---|
HeLa | A549 | HCT-116 | HepG-2 | HUVEC | |
1 | 1.59 | 1.55 | 0.64 | 0.78 | 0.72 |
2 | 11.50 | 4.76 | 4.76 | 6.35 | 4.12 |
3 | 13.78 | 14.23 | 8.89 | 13.34 | 6.14 |
4 | 7.58 | 11.30 | 4.49 | 10.21 | 2.48 |
5 | 5.33 | 6.43 | 2.76 | 5.97 | 2.39 |
6 | 4.07 | 4.88 | 1.47 | 2.44 | 2.12 |
7 | 3.26 | 4.88 | 1.30 | 6.51 | 1.04 |
8 | 4.77 | 2.86 | 1.59 | 6.37 | 0.91 |
9 | 6.35 | 12.69 | 11.74 | 6.35 | 1.73 |
10 | 2.60 | 2.05 | 1.20 | 1.00 | 0.90 |
11 | 2.95 | 1.80 | 1.35 | 1.15 | 1.60 |
Xanthone | Antiangiogenic Phenotype | |||||
---|---|---|---|---|---|---|
1 μM | 2 μM | 4 μM | 8 μM | 16 μM | 32 μM | |
1 | √ | √ | ||||
2 | × | × | ||||
3 | × | × | √ | |||
4 | × | × | × | × | ||
5 | × | × | × | |||
6 | × | × | × | × | × | × |
7 | × | × | × | × | √ | √ |
8 | × | × | × | × | ||
9 | × | × | √ | |||
10 | × | × | × | × | ||
11 | × | × | × | × |
Xanthone | Death Rate | |||||
---|---|---|---|---|---|---|
1 μM | 2 μM | 4 μM | 8 μM | 16 μM | 32 μM | |
1 | 0 | 1/8 | 8/8 | |||
2 | 0 | 0 | 8/8 | |||
3 | 0 | 0 | 8/8 | |||
4 | 0 | 0 | 0 | |||
5 | 0 | 0 | 8/8 | |||
6 | 0 | 1/8 | 4/8 | |||
7 | 0 | 0 | 0 | |||
8 | 0 | 1/8 | 6/8 | |||
9 | 0 | 0 | 8/8 | |||
10 | 0 | 0 | 0 | 0 | ||
11 | 0 | 0 | 0 | 0 |
Xanthone | Heart Rate (per 10 s) | |||||
---|---|---|---|---|---|---|
1 μM | 2 μM | 4 μM | 8 μM | 16 μM | 32 μM | |
1 | 8 | 3 | 0 | |||
2 | 21 | 18 | 0 | |||
3 | 8 | 6 | 0 | |||
4 | 21 | 17 | 13 | |||
5 | 13 | 10 | 8 | |||
6 | 17 | 14 | 8 | |||
7 | 6 | 6 | 5 | |||
8 | 17 | 13 | 6 | |||
9 | 8 | 5 | 0 | |||
10 | 20 | 17 | 16 | 16 | ||
11 | 21 | 21 | 21 | 20 |
3. Experimental
3.1. Plant Material
3.2. Isolation
3.3. MTT Assay
3.4. Antiangiogenic Activity Assay on Blood Vessel Formation in Zebrafish Embryos
3.5. HUVEC Wound Migration Assay
4. Conclusion
Acknowledgments
Conflicts of Interest
References
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Yang, J.; He, S.; Li, S.; Zhang, R.; Peng, A.; Chen, L. In Vitro and In Vivo Antiangiogenic Activity of Caged Polyprenylated Xanthones Isolated from Garcinia hanburyi Hook. f. Molecules 2013, 18, 15305-15313. https://doi.org/10.3390/molecules181215305
Yang J, He S, Li S, Zhang R, Peng A, Chen L. In Vitro and In Vivo Antiangiogenic Activity of Caged Polyprenylated Xanthones Isolated from Garcinia hanburyi Hook. f. Molecules. 2013; 18(12):15305-15313. https://doi.org/10.3390/molecules181215305
Chicago/Turabian StyleYang, Jianhong, Shichao He, Shucai Li, Ronghong Zhang, Aihua Peng, and Lijuan Chen. 2013. "In Vitro and In Vivo Antiangiogenic Activity of Caged Polyprenylated Xanthones Isolated from Garcinia hanburyi Hook. f." Molecules 18, no. 12: 15305-15313. https://doi.org/10.3390/molecules181215305