Molecules 2013, 18(12), 14935-14947; doi:10.3390/molecules181214935
Article

Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells

1,†email, 2,†email, 2,†email, 3,4email and 3,4,* email
1 Department of Science and Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 202032, China 2 Department of Oncology, Center Hospital of Nanyang, Nanyang, Henan 473000, China 3 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 202032, China 4 Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 202032, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 November 2013; in revised form: 27 November 2013 / Accepted: 27 November 2013 / Published: 4 December 2013
PDF Full-text Download PDF Full-Text [1571 KB, uploaded 4 December 2013 11:59 CET]
Abstract: Hepatocarcinogenesis is associated with epigenetic changes, including histone deacetylases (HDACs). Epigenetic modulation by HDAC inhibition is a potentially valuable approach for hepatocellular carcinoma treatment. In present study, we evaluated the anticancer effects of sodium valproate (SVP), a known HDAC inhibitor, in human hepatocarcinoma cells. The results showed SVP inhibited the proliferation of Bel-7402 cells in a dose-dependent manner. Low dose SVP treatment caused a large and flat morphology change, positive SA-β-gal staining, and G0/G1 phase cell cycle arrest in human hepatocarcinoma cells. Low dose SVP treatment also increased acetylation of histone H3 and H4 on p21 promoter, accompanied by up-regulation of p21 and down-regulation of RB phosphorylation. These observations suggested that a low dose of SVP could induce cell senescence in hepatocarcinoma cells, which might correlate with hyperacetylation of histone H3 and H4, up-regulation of p21, and inhibition of RB phosphorylation. Since the effective concentration inducing cell senescence in hepatocarcinoma cells is clinically available, whether a clinical dose of SVP could induce cell senescence in clinical hepatocarcinoma is worthy of further study.
Keywords: hepatocarcinoma; valproic acid sodium salt; cell senescence

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

An, H.-M.; Xue, Y.-F.; Shen, Y.-L.; Du, Q.; Hu, B. Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells. Molecules 2013, 18, 14935-14947.

AMA Style

An H-M, Xue Y-F, Shen Y-L, Du Q, Hu B. Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells. Molecules. 2013; 18(12):14935-14947.

Chicago/Turabian Style

An, Hong-Mei; Xue, Yong-Fei; Shen, Yan-Li; Du, Qin; Hu, Bing. 2013. "Sodium Valproate Induces Cell Senescence in Human Hepatocarcinoma Cells." Molecules 18, no. 12: 14935-14947.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert