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Molecules 2013, 18(12), 14862-14875; doi:10.3390/molecules181214862
Article

Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia

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1 Division of Hemato-Oncology, Department of Internal Medicine, Yuan's General Hospital, No.162 Cheng Kung 1st Road, Kaohsiung City 80249, Taiwan 2 Program of Health-Business Administration, School of Nursing, Fooyin University, No.151 Jinxue Road, Kaohsiung City 83102, Taiwan 3 Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, No.155 Section 2, Linong Street, Taipei City 11221, Taiwan 4 Department of Education and Research, Taipei City Hospital Renai Branch, No.10, Section 4, Renai Road, Taipei City 10629, Taiwan 5 School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan 6 Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No.300 Syuefu Road, Chiayi City 60004, Taiwan 7 School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
* Author to whom correspondence should be addressed.
Received: 30 September 2013 / Revised: 27 November 2013 / Accepted: 28 November 2013 / Published: 3 December 2013
(This article belongs to the Section Metabolites)
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Abstract

Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity.  Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.
Keywords: Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix); Fabaceae; Astragali Radix; anti-inflammation; splenocyte proliferation; daunoblastina-induced leucopenia mice Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix); Fabaceae; Astragali Radix; anti-inflammation; splenocyte proliferation; daunoblastina-induced leucopenia mice
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, G.-C.; Lee, C.-J.; Wang, K.-T.; Weng, B.-C.; Chien, T.-Y.; Tseng, S.-H.; Wang, C.-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules 2013, 18, 14862-14875.

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