Abstract: Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity. Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.
Keywords: Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix); Fabaceae; Astragali Radix; anti-inflammation; splenocyte proliferation; daunoblastina-induced leucopenia mice
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Huang, G.-C.; Lee, C.-J.; Wang, K.-T.; Weng, B.-C.; Chien, T.-Y.; Tseng, S.-H.; Wang, C.-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules 2013, 18, 14862-14875.
Huang G-C, Lee C-J, Wang K-T, Weng B-C, Chien T-Y, Tseng S-H, Wang C-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules. 2013; 18(12):14862-14875.
Huang, Guan-Cheng; Lee, Chia-Jung; Wang, Kun-Teng; Weng, Bor-Chun; Chien, Ting-Yi; Tseng, Sung-Hui; Wang, Ching-Chiung. 2013. "Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia." Molecules 18, no. 12: 14862-14875.