Molecules 2013, 18(12), 14862-14875; doi:10.3390/molecules181214862
Article

Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia

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Received: 30 September 2013; in revised form: 27 November 2013 / Accepted: 28 November 2013 / Published: 3 December 2013
(This article belongs to the Section Metabolites)
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Abstract: Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity.  Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.
Keywords: Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix); Fabaceae; Astragali Radix; anti-inflammation; splenocyte proliferation; daunoblastina-induced leucopenia mice
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Huang, G.-C.; Lee, C.-J.; Wang, K.-T.; Weng, B.-C.; Chien, T.-Y.; Tseng, S.-H.; Wang, C.-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules 2013, 18, 14862-14875.

AMA Style

Huang G-C, Lee C-J, Wang K-T, Weng B-C, Chien T-Y, Tseng S-H, Wang C-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules. 2013; 18(12):14862-14875.

Chicago/Turabian Style

Huang, Guan-Cheng; Lee, Chia-Jung; Wang, Kun-Teng; Weng, Bor-Chun; Chien, Ting-Yi; Tseng, Sung-Hui; Wang, Ching-Chiung. 2013. "Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia." Molecules 18, no. 12: 14862-14875.


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