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Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia
Division of Hemato-Oncology, Department of Internal Medicine, Yuan's General Hospital, No.162 Cheng Kung 1st Road, Kaohsiung City 80249, Taiwan
Program of Health-Business Administration, School of Nursing, Fooyin University, No.151 Jinxue Road, Kaohsiung City 83102, Taiwan
Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, No.155 Section 2, Linong Street, Taipei City 11221, Taiwan
Department of Education and Research, Taipei City Hospital Renai Branch, No.10, Section 4, Renai Road, Taipei City 10629, Taiwan
School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No.300 Syuefu Road, Chiayi City 60004, Taiwan
School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
* Author to whom correspondence should be addressed.
Received: 30 September 2013; in revised form: 27 November 2013 / Accepted: 28 November 2013 / Published: 3 December 2013
Abstract: Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity. Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.
Keywords: Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix); Fabaceae; Astragali Radix; anti-inflammation; splenocyte proliferation; daunoblastina-induced leucopenia mice
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MDPI and ACS Style
Huang, G.-C.; Lee, C.-J.; Wang, K.-T.; Weng, B.-C.; Chien, T.-Y.; Tseng, S.-H.; Wang, C.-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules 2013, 18, 14862-14875.
Huang G-C, Lee C-J, Wang K-T, Weng B-C, Chien T-Y, Tseng S-H, Wang C-C. Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia. Molecules. 2013; 18(12):14862-14875.
Huang, Guan-Cheng; Lee, Chia-Jung; Wang, Kun-Teng; Weng, Bor-Chun; Chien, Ting-Yi; Tseng, Sung-Hui; Wang, Ching-Chiung. 2013. "Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia." Molecules 18, no. 12: 14862-14875.