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Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection
College of Enology, Northwest A&F University, Yangling 712100, Shaanxi, China
Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, Shaanxi, China
College of Plant Science, Tarim University, Alaer 843300, Xinjiang, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 September 2013; in revised form: 7 November 2013 / Accepted: 13 November 2013 / Published: 18 November 2013
Abstract: The paper described a novel chromatographic method for the simultaneous determination of phenolic compounds such as gallic, protocatechuic, vanillic, caffeic, syringic, p-coumaric and salicylic acid, (+)-catechin, (‒)-epicatechin, rutin, morin, quercetin, coumarin and trans-resveratrol at their maximum absorbance wavelengths (MAW) employing reverse-phase high performance liquid chromatography combined with DAD and UV detection via detection wavelength switching. The method was based on MAW acquisition by DAD and quantification by UV. The separation process was performed on a Shim-Pack VP-ODS C18 column (250 mm × 4.6 mm, 5 μm) held at 30 °C, utilizing 3.0% acetic acid and acetonitrile as mobile phase at a flow rate of 0.8 mL/min in the gradient elution mode. The method was fully validated in terms of linearity (r2 > 0.9990, 10‒350 mg/L), precision (both intra-day and inter-day RSD < 4.22%), accuracy (97.31%‒104.66%), specificity, robustness (0.59% < RSD < 2.86%), limit of detection and quantification. The switching method significantly improved the sensitivities of most phenolics studied in comparison with the standard constant wavelength detection (280 nm). The proposed method has been successfully applied to the determination of 14 phenolic compounds in 89 varieties of one-year-old Chinese grape one-year-canes. Grape canes contain many phenolics, especially trans-resveratrol, (‒)-epicatechin, and (+)-catechin.
Keywords: HPLC-DAD-UV; wavelength switching; phenolic compounds; grape canes
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MDPI and ACS Style
Zhang, A.; Wan, L.; Wu, C.; Fang, Y.; Han, G.; Li, H.; Zhang, Z.; Wang, H. Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection. Molecules 2013, 18, 14241-14257.
Zhang A, Wan L, Wu C, Fang Y, Han G, Li H, Zhang Z, Wang H. Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection. Molecules. 2013; 18(11):14241-14257.
Zhang, Ang; Wan, Li; Wu, Cuiyun; Fang, Yulin; Han, Guomin; Li, Hua; Zhang, Zhenwen; Wang, Hua. 2013. "Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection." Molecules 18, no. 11: 14241-14257.