Molecules 2013, 18(11), 14241-14257; doi:10.3390/molecules181114241
Article

Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection

1,2,†email, 1,2,†email, 3email, 1,2,* email, 1,2email, 1,2email, 1,2email and 1,2email
1 College of Enology, Northwest A&F University, Yangling 712100, Shaanxi, China 2 Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, Shaanxi, China 3 College of Plant Science, Tarim University, Alaer 843300, Xinjiang, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 18 September 2013; in revised form: 7 November 2013 / Accepted: 13 November 2013 / Published: 18 November 2013
PDF Full-text Download PDF Full-Text [594 KB, uploaded 18 November 2013 12:19 CET]
Abstract: The paper described a novel chromatographic method for the simultaneous determination of phenolic compounds such as gallic, protocatechuic, vanillic, caffeic, syringic, p-coumaric and salicylic acid, (+)-catechin, (‒)-epicatechin, rutin, morin, quercetin, coumarin and trans-resveratrol at their maximum absorbance wavelengths (MAW) employing reverse-phase high performance liquid chromatography combined with DAD and UV detection via detection wavelength switching. The method was based on MAW acquisition by DAD and quantification by UV. The separation process was performed on a Shim-Pack VP-ODS C18 column (250 mm × 4.6 mm, 5 μm) held at 30 °C, utilizing 3.0% acetic acid and acetonitrile as mobile phase at a flow rate of 0.8 mL/min in the gradient elution mode. The method was fully validated in terms of linearity (r2 > 0.9990, 10‒350 mg/L), precision (both intra-day and inter-day RSD < 4.22%), accuracy (97.31%‒104.66%), specificity, robustness (0.59% < RSD < 2.86%), limit of detection and quantification. The switching method significantly improved the sensitivities of most phenolics studied in comparison with the standard constant wavelength detection (280 nm). The proposed method has been successfully applied to the determination of 14 phenolic compounds in 89 varieties of one-year-old Chinese grape one-year-canes. Grape canes contain many phenolics, especially trans-resveratrol, (‒)-epicatechin, and (+)-catechin.
Keywords: HPLC-DAD-UV; wavelength switching; phenolic compounds; grape canes

Supplementary Files

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Zhang, A.; Wan, L.; Wu, C.; Fang, Y.; Han, G.; Li, H.; Zhang, Z.; Wang, H. Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection. Molecules 2013, 18, 14241-14257.

AMA Style

Zhang A, Wan L, Wu C, Fang Y, Han G, Li H, Zhang Z, Wang H. Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection. Molecules. 2013; 18(11):14241-14257.

Chicago/Turabian Style

Zhang, Ang; Wan, Li; Wu, Cuiyun; Fang, Yulin; Han, Guomin; Li, Hua; Zhang, Zhenwen; Wang, Hua. 2013. "Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection." Molecules 18, no. 11: 14241-14257.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert