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Molecules 2013, 18(11), 13435-13445; doi:10.3390/molecules181113435

Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability

1
Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G.Masaryka-5555, Zlín 76001, Czech Republic
2
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. sq. 275, Zlin 76272, Czech Republic
3
Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlin, nám. T.G.Masaryka-5555, Zlin 76272, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 30 August 2013 / Revised: 3 October 2013 / Accepted: 23 October 2013 / Published: 30 October 2013
(This article belongs to the Section Natural Products)
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Abstract

The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae), introduced Sage (Salvia pratensis, Lamiaceae), European elderberry (Sambucus nigra, Caprifoliaceae) and common dandelion (Taraxacum officinale, Asteraceae) were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen. View Full-Text
Keywords: edible flowers; polyphenolic extracts; atelocollagen thin films; cell viability; tissue engineering edible flowers; polyphenolic extracts; atelocollagen thin films; cell viability; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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López-García, J.; Kuceková, Z.; Humpolíček, P.; Mlček, J.; Sáha, P. Polyphenolic Extracts of Edible Flowers Incorporated onto Atelocollagen Matrices and Their Effect on Cell Viability. Molecules 2013, 18, 13435-13445.

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