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Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions
Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan
Division of Chemistry, Department of Medical Sciences Faculty of Medicine, University of Miyazaki 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, and Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889-1692, Japan
* Author to whom correspondence should be addressed.
Received: 1 August 2013; in revised form: 10 October 2013 / Accepted: 13 October 2013 / Published: 17 October 2013
Abstract: 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.
Keywords: click chemistry; cAMP; fluorescence; cAMP localization in living cells
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MDPI and ACS Style
Ito, K.; Liu, H.; Komiyama, M.; Hayashi, T.; Xu, Y. Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions. Molecules 2013, 18, 12909-12915.
Ito K, Liu H, Komiyama M, Hayashi T, Xu Y. Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions. Molecules. 2013; 18(10):12909-12915.
Ito, Kenichiro; Liu, Hongshan; Komiyama, Makoto; Hayashi, Tetsuya; Xu, Yan. 2013. "Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions." Molecules 18, no. 10: 12909-12915.