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Molecules 2013, 18(10), 12909-12915; doi:10.3390/molecules181012909
Article

Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

1, 2, 1, 3 and 2,*
Received: 1 August 2013; in revised form: 10 October 2013 / Accepted: 13 October 2013 / Published: 17 October 2013
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Abstract: 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.
Keywords: click chemistry; cAMP; fluorescence; cAMP localization in living cells click chemistry; cAMP; fluorescence; cAMP localization in living cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ito, K.; Liu, H.; Komiyama, M.; Hayashi, T.; Xu, Y. Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions. Molecules 2013, 18, 12909-12915.

AMA Style

Ito K, Liu H, Komiyama M, Hayashi T, Xu Y. Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions. Molecules. 2013; 18(10):12909-12915.

Chicago/Turabian Style

Ito, Kenichiro; Liu, Hongshan; Komiyama, Makoto; Hayashi, Tetsuya; Xu, Yan. 2013. "Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions." Molecules 18, no. 10: 12909-12915.


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