Molecules 2013, 18(10), 12896-12908; doi:10.3390/molecules181012896
Article

Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by High-Speed Counter-Current Chromatography

1, 1, 1, 1, 1, 2, 1 and 1,* email
Received: 8 July 2013; in revised form: 29 September 2013 / Accepted: 12 October 2013 / Published: 16 October 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: High-speed counter-current chromatography (HSCCC) was applied for the first time for the preparative separation of spirobisnaphthalenes from a crude extract of the endophytic fungus Berkleasmium sp. Dzf12, associated with the medicinal plant Dioscorea zingiberensis. Six spirobisnaphthalenes were successfully separated by HSCCC with a two-phase solvent system composed of n-hexane-chloroform-methanol-water (1.5:3.0:2.5:2.0, v/v). About 18.0 mg of diepoxin k (1), 245.7 mg of palmarumycin C13 (2), 42.4 mg of palmarumycin C16 (3), 42.2 mg of palmarumycin C15 (4), 32.6 mg of diepoxin δ (5), and 22.3 mg of diepoxin γ (6) with purities of 56.82, 71.39, 76.57, 75.86, 91.01 and 82.48%, respectively, as determined by high-performance liquid chromatography (HPLC), were obtained from 500 mg of the crude extract in a one-step elution within 7 h of separation procedure by HSCCC. The purified spirobisnaphthalenes were further structurally characterized by means of physicochemical and spectrometric analysis.
Keywords: preparative separation; spirobisnaphthalenes; endophytic fungus; Berkleasmium sp. Dzf12; high-speed counter-current chromatogrphy
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MDPI and ACS Style

Shan, T.; Lu, S.; Luo, C.; Luo, R.; Mou, Y.; Wang, M.; Peng, Y.; Zhou, L. Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by High-Speed Counter-Current Chromatography. Molecules 2013, 18, 12896-12908.

AMA Style

Shan T, Lu S, Luo C, Luo R, Mou Y, Wang M, Peng Y, Zhou L. Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by High-Speed Counter-Current Chromatography. Molecules. 2013; 18(10):12896-12908.

Chicago/Turabian Style

Shan, Tijiang; Lu, Shiqiong; Luo, Chao; Luo, Ruiya; Mou, Yan; Wang, Mingan; Peng, Youliang; Zhou, Ligang. 2013. "Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by High-Speed Counter-Current Chromatography." Molecules 18, no. 10: 12896-12908.

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