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Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates
Jozef Stefan International Postgraduate School, Jamova 39, Ljubljana 1000, Slovenia
Anti Doping Laboratory Qatar, Doha, 27775, State of Qatar
Rudjer Boskovic Institute, Bijenicka 54, Zagreb 10000, Croatia
Plasma Laboratory, Institute Jozef Stefan, Jamova 39, Ljubljana 1000, Slovenia
Laboratory for Characterization and Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, Maribor 2000, Slovenia
* Author to whom correspondence should be addressed.
Received: 2 August 2013; in revised form: 30 September 2013 / Accepted: 8 October 2013 / Published: 10 October 2013
Abstract: Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
Keywords: oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)
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Recek, N.; Jaganjac, M.; Kolar, M.; Milkovic, L.; Mozetič, M.; Stana-Kleinschek, K.; Vesel, A. Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates. Molecules 2013, 18, 12441-12463.
Recek N, Jaganjac M, Kolar M, Milkovic L, Mozetič M, Stana-Kleinschek K, Vesel A. Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates. Molecules. 2013; 18(10):12441-12463.
Recek, Nina; Jaganjac, Morana; Kolar, Metod; Milkovic, Lidija; Mozetič, Miran; Stana-Kleinschek, Karin; Vesel, Alenka. 2013. "Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates." Molecules 18, no. 10: 12441-12463.