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Molecules 2013, 18(10), 12441-12463; doi:10.3390/molecules181012441
Article

Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates

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1 Jozef Stefan International Postgraduate School, Jamova 39, Ljubljana 1000, Slovenia 2 Anti Doping Laboratory Qatar, Doha, 27775, State of Qatar 3 Rudjer Boskovic Institute, Bijenicka 54, Zagreb 10000, Croatia 4 Plasma Laboratory, Institute Jozef Stefan, Jamova 39, Ljubljana 1000, Slovenia 5 Laboratory for Characterization and Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, Maribor 2000, Slovenia
* Author to whom correspondence should be addressed.
Received: 2 August 2013 / Revised: 30 September 2013 / Accepted: 8 October 2013 / Published: 10 October 2013
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Abstract

Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
Keywords: oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM) oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Recek, N.; Jaganjac, M.; Kolar, M.; Milkovic, L.; Mozetič, M.; Stana-Kleinschek, K.; Vesel, A. Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates. Molecules 2013, 18, 12441-12463.

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