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Molecules 2013, 18(10), 12441-12463; doi:10.3390/molecules181012441
Article

Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates

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Received: 2 August 2013 / Revised: 30 September 2013 / Accepted: 8 October 2013 / Published: 10 October 2013
Download PDF [2443 KB, 18 June 2014; original version 18 June 2014]

Abstract

Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
Keywords: oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM) oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Recek, N.; Jaganjac, M.; Kolar, M.; Milkovic, L.; Mozetič, M.; Stana-Kleinschek, K.; Vesel, A. Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates. Molecules 2013, 18, 12441-12463.

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