Molecules 2013, 18(10), 12154-12167; doi:10.3390/molecules181012154

Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats

1, 1, 1, 1, 1, 1, 2, 3, 4, 5, 6, 2,7 and 8,* email
Received: 1 July 2013; in revised form: 18 September 2013 / Accepted: 18 September 2013 / Published: 30 September 2013
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.
Keywords: acute radiation syndrome; black grape juice; heart; oxidative damage; lactate dehydrogenase; phenolics; flavonoids; HPLC-DAD
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MDPI and ACS Style

de Freitas, R.B.; Boligon, A.A.; Rovani, B.T.; Piana, M.; de Brum, T.F.; da Silva Jesus, R.; Rother, F.C.; Alves, N.M.; Teixeira da Rocha, J.B.; Athayde, M.L.; Barrio, J.P.; de Andrade, E.R.; de Freitas Bauerman, L. Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats. Molecules 2013, 18, 12154-12167.

AMA Style

de Freitas RB, Boligon AA, Rovani BT, Piana M, de Brum TF, da Silva Jesus R, Rother FC, Alves NM, Teixeira da Rocha JB, Athayde ML, Barrio JP, de Andrade ER, de Freitas Bauerman L. Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats. Molecules. 2013; 18(10):12154-12167.

Chicago/Turabian Style

de Freitas, Robson B.; Boligon, Aline A.; Rovani, Bruno T.; Piana, Mariana; de Brum, Thiele F.; da Silva Jesus, Roberta; Rother, Fagner C.; Alves, Nelson M.; Teixeira da Rocha, João B.; Athayde, Margareth L.; Barrio, Juan P.; de Andrade, Edson R.; de Freitas Bauerman, Liliane. 2013. "Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats." Molecules 18, no. 10: 12154-12167.

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