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Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats
Postgraduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil
Nuclear Engineering Program, Military Institute of Engineering (IME), Rio de Janeiro, RJ, 22290-270, Brazil
Postgraduate Program, Institute of Nuclear and Energy Research of University of São Paulo (IPEN), São Paulo, SP, 05508-000, Brazil
Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil
Department of Industrial Pharmacy, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil
Department of Biomedical Sciences and Institute of Biomedicine (IBIOMED), University of León, León 24071, Spain
Brazilian Army Technological Center (CTEx), Chemical, Biological and Nuclear Defense Division, Rio de Janeiro, RJ, 23020-470, Brazil
Department of Physiology and Pharmacology, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil
* Author to whom correspondence should be addressed.
Received: 1 July 2013; in revised form: 18 September 2013 / Accepted: 18 September 2013 / Published: 30 September 2013
Abstract: The aim of this study was to evaluate the potential positive effect of black grape juice (BGJ) on lipid peroxidation considering Total Body Irradiation (TBI) in Wistar rats. As a potential feasible means of evaluation in situ, blood serum lactate dehydrogenase (LDH) levels were evaluated as a marker for heart damage from acute radiation syndrome (ARS). Twenty rats were divided into four groups, two of them being irradiated by gamma-rays from a Co-60 source. Animals were treated by gavage with 2 mL per day of BGJ or placebo for one week before and 4 days after 6 Gy whole body gamma-irradiation, when they were euthanasiated. LDH on serum and lipid peroxidation on heart tissue were evaluated. High concentration of metabolites from lipid peroxidation in heart, and high LDH level on serum were found only in gamma-irradiated group given placebo, mainly at the first 24 h after radiation. Phytochemical analysis of BGJ was performed by determining total phenolics, flavonoids, and tannins followed by a high-performance liquid chromatography (HPLC/DAD) analysis, which showed resveratrol as the major constituent. Results suggest that BGJ is a good protective candidate compound against heart damage from ARS and its effects suggest its use as a radiomodifier.
Keywords: acute radiation syndrome; black grape juice; heart; oxidative damage; lactate dehydrogenase; phenolics; flavonoids; HPLC-DAD
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de Freitas, R.B.; Boligon, A.A.; Rovani, B.T.; Piana, M.; de Brum, T.F.; da Silva Jesus, R.; Rother, F.C.; Alves, N.M.; Teixeira da Rocha, J.B.; Athayde, M.L.; Barrio, J.P.; de Andrade, E.R.; de Freitas Bauerman, L. Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats. Molecules 2013, 18, 12154-12167.
de Freitas RB, Boligon AA, Rovani BT, Piana M, de Brum TF, da Silva Jesus R, Rother FC, Alves NM, Teixeira da Rocha JB, Athayde ML, Barrio JP, de Andrade ER, de Freitas Bauerman L. Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats. Molecules. 2013; 18(10):12154-12167.
de Freitas, Robson B.; Boligon, Aline A.; Rovani, Bruno T.; Piana, Mariana; de Brum, Thiele F.; da Silva Jesus, Roberta; Rother, Fagner C.; Alves, Nelson M.; Teixeira da Rocha, João B.; Athayde, Margareth L.; Barrio, Juan P.; de Andrade, Edson R.; de Freitas Bauerman, Liliane. 2013. "Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats." Molecules 18, no. 10: 12154-12167.