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4862F, a New Inhibitor of HIV-1 Protease, from the Culture of Streptomyces I03A-04862
AbstractWe have isolated an extraordinary pentapeptide, called 4862F, from the culture broth of Streptomyces albosporus I03A-04862 by Diaion HP-20 macroporous adsorbent resin column, ODS-A and Sephadex LH-20 chromatography, followed by preparative HPLC. This peptide shows inhibitory activity against HIV-1 protease. The structure was elucidated by spectroscopic approaches, including ESI-MS and various NMR methods. Absolute configuration of the amino acid residues in 4862F was defined using Marfey’s method, and the structure was identified as N,N,N-(trimethylated)-Tyr-L-Leu-L-Val-L-Leu-(dehydrated)-His. The peptide 4862F displays inhibitory activity against HIV-1 protease, with IC50 values of 15.26 nM, using a fluorescence-based assay.
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Liu, X.; Gan, M.; Dong, B.; Zhang, T.; Li, Y.; Zhang, Y.; Fan, X.; Wu, Y.; Bai, S.; Chen, M.; Yu, L.; Tao, P.; Jiang, W.; Si, S. 4862F, a New Inhibitor of HIV-1 Protease, from the Culture of Streptomyces I03A-04862. Molecules 2013, 18, 236-243.View more citation formats
Liu X, Gan M, Dong B, Zhang T, Li Y, Zhang Y, Fan X, Wu Y, Bai S, Chen M, Yu L, Tao P, Jiang W, Si S. 4862F, a New Inhibitor of HIV-1 Protease, from the Culture of Streptomyces I03A-04862. Molecules. 2013; 18(1):236-243.Chicago/Turabian Style
Liu, Xiao; Gan, Maoluo; Dong, Biao; Zhang, Tian; Li, Yongzhen; Zhang, Yuqin; Fan, Xiuyong; Wu, Yexiang; Bai, Shuoke; Chen, Minghua; Yu, Liyan; Tao, Peizhen; Jiang, Wei; Si, Shuyi. 2013. "4862F, a New Inhibitor of HIV-1 Protease, from the Culture of Streptomyces I03A-04862." Molecules 18, no. 1: 236-243.