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Molecules 2013, 18(1), 1138-1149; doi:10.3390/molecules18011138
Article

Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA

1,2,†, 1,3,†, 1, 1 and 1,*
Received: 3 December 2012; in revised form: 9 January 2013 / Accepted: 10 January 2013 / Published: 16 January 2013
(This article belongs to the Section Medicinal Chemistry)
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Abstract: The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal conditions, the immobilized trypsin displays maximum specific activity (49.8 μmol/min/g). The results also indicate that the immobilized trypsin exhibits better storage stability.
Keywords: SBA-15 mesoporous silica; trypsin; immobilization; specific activity; hydrolysis; BAPNA SBA-15 mesoporous silica; trypsin; immobilization; specific activity; hydrolysis; BAPNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, S.; Wu, Z.; Lu, M.; Wang, Z.; Li, Z. Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA. Molecules 2013, 18, 1138-1149.

AMA Style

Li S, Wu Z, Lu M, Wang Z, Li Z. Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA. Molecules. 2013; 18(1):1138-1149.

Chicago/Turabian Style

Li, Shanshan; Wu, Zhuofu; Lu, Ming; Wang, Zhi; Li, Zhengqiang. 2013. "Improvement of the Enzyme Performance of Trypsin via Adsorption in Mesoporous Silica SBA-15: Hydrolysis of BAPNA." Molecules 18, no. 1: 1138-1149.



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