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Primary Investigation of the Preparation of Nanoparticles by Precipitation
Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1/3, 612 42 Brno, Czech Republic
Nanotechnology Centre, VSB—Technical University of Ostrava, 17. listopadu 15/2172, 708 33 Ostrava, Czech Republic
Institute of Chemical Technology, Faculty of Chemical Engineering, Technicka 5, 166 28 Prague 6, Czech Republic
Institute of Inorganic Chemistry, Academy of Science, 25068 Rez, Czech Republic
Research Institute for Pharmacy and Biochemistry, Lidicka 1879/48, 602 00 Brno, Czech Republic
* Authors to whom correspondence should be addressed.
Received: 20 August 2012; in revised form: 21 August 2012 / Accepted: 3 September 2012 / Published: 13 September 2012
Abstract: The absorption, distribution, biotransformation and excretion of a drug involve its transport across cell membranes. This process is essential and influenced by the characteristics of the drug, especially its molecular size and shape, solubility at the site of its absorption, relative lipid solubility, etc. One of the progressive ways for increasing bioavaibility is a nanoparticle preparation technique. Cholesterol, cholestenolone and pregnenolone acetate as model active pharmaceutical ingredients and some of the commonly used excipients as nanoparticle stabilizers were used in the investigated precipitation method that was modified and simplified and can be used as an effective and an affordable technique for the preparation of nanoparticles. All 120 prepared samples were analyzed by means of dynamic light scattering (Nanophox). The range of the particle size of the determined 100 nanoparticle samples was from 1 nm to 773 nm, whereas 82 samples contained nanoparticles of less than 200 nm. Relationships between solvents and used excipients and their amount are discussed.
Keywords: steroids; nanoparticles; precipitation; excipients; dynamic light scattering
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Vaculikova, E.; Grunwaldova, V.; Kral, V.; Dohnal, J.; Jampilek, J. Primary Investigation of the Preparation of Nanoparticles by Precipitation. Molecules 2012, 17, 11067-11078.
Vaculikova E, Grunwaldova V, Kral V, Dohnal J, Jampilek J. Primary Investigation of the Preparation of Nanoparticles by Precipitation. Molecules. 2012; 17(9):11067-11078.
Vaculikova, Eliska; Grunwaldova, Veronika; Kral, Vladimir; Dohnal, Jiri; Jampilek, Josef. 2012. "Primary Investigation of the Preparation of Nanoparticles by Precipitation." Molecules 17, no. 9: 11067-11078.