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Molecules 2012, 17(7), 8617-8632; doi:10.3390/molecules17078617
Article

Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish

1
, 2,* , 1
, 1
, 1
, 1,* , 3
 and 3
1 Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Street, Nanjing 210028, China 2 State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, China 3 Biology & Medicine Science and Technology Industry Park of Weifang Hi-Tech Development Zone, Crossroads of Jiankang East Street and Gaoxin Road 2, Weifang 261061, China
* Authors to whom correspondence should be addressed.
Received: 25 June 2012 / Revised: 9 July 2012 / Accepted: 10 July 2012 / Published: 18 July 2012
(This article belongs to the Section Medicinal Chemistry)
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Abstract

The study aimed to investigate the potential of zebrafish in imitating mammal phase I metabolism of natural compounds. Three diterpenoid quinones from Radix Salvia miltiorrhiza, namely tanshinone IIA (TIIA), cryptotanshinone (Cry) and tanshinone I (TI) were selected as model compounds, and their metabolites mediated by zebrafish were characterized using a high-performance liquid chromatography coupled ion-trap mass spectrometry (HPLC/IT-MSn) method with electrospray ionization in positive mode. The separation was performed with a Zorbax C-18 column using a binary gradient elution of 0.05% formic acid acetonitrile/0.05% formic acid water. According to the MS spectra and after comparison with reference standards and literature reports, hydroxylation, dehydrogenation or D-ring hydrolysis metabolites of TIIA and Cry but not of TI were characterized, which coincided with those reported using regular in vivo or in vitro metabolic analysis methods, thus verifying that zebrafish can successfully imitate mammalian phase I metabolism which instills further confidence in using zebrafish as a novel and prospective metabolism model.
Keywords: zebrafish; phase I metabolism; tanshinone IIA; cryptotanshinone; tanshinone I; Radix Salvia miltiorrhiza zebrafish; phase I metabolism; tanshinone IIA; cryptotanshinone; tanshinone I; Radix Salvia miltiorrhiza
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wei, Y.; Li, P.; Wang, C.; Peng, Y.; Shu, L.; Jia, X.; Ma, W.; Wang, B. Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish. Molecules 2012, 17, 8617-8632.

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