Molecules 2012, 17(7), 8633-8646; doi:10.3390/molecules17078633
Article

Anti-Hepatitis B Virus Activity of Chickweed [Stellaria media (L.) Vill.] Extracts in HepG2.2.15 Cells

Lihua Ma 1,2 email, Jie Song 1 email, Yaqin Shi 1 email, Changmei Wang 1 email, Bin Chen 1 email, Donghao Xie 2 email and Xiaobin Jia 1,* email
1 Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Street, Nanjing 210028, China 2 School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, 212013, China
* Author to whom correspondence should be addressed.
Received: 22 May 2012; in revised form: 29 June 2012 / Accepted: 9 July 2012 / Published: 18 July 2012
(This article belongs to the Section Natural Products)
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Abstract: Stellaria media (Linn.) Villars is a traditional Chinese medicine that has been used for over 200 years, mainly for the treatment of dermatitis and other skin diseases. It has also been used as an anti-viral agent. All the fresh chickweed juice samples used in this study were prepared using macroporous resin and ultrafiltration technology. The anti-hepatitis B virus (HBV) activity of S. media was evaluated in vitro using the human HBV-transfected liver cell line HepG2.2.15. The concentrations of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in HepG2.2.15 cell culture medium were determined by enzyme-linked immunosorbent assay (ELISA) after S. media-n (SM-n) treatment for 6 or 9 days. HBV DNA was quantified using transcription-mediated amplification and real-time polymerase chain reaction. In HepG2.2.15 cells, 30 μg/mL SM-3 effectively suppressed the secretion of HBsAg and HBeAg with inhibition rates of 27.92% and 25.35% after 6 days of treatment, respectively. Consistent with the reduction in HBV antigens, SM-3 also reduced the level of HBV DNA in a dose-dependent manner. The characterization and quantitation of the chemical composition of SM-3 showed the presence of flavonoid C-glycosides, polysaccharides, and protein, which exhibited diverse antiviral activities. In conclusion, our results demonstrate that SM-3 possesses potential anti-HBV activity in vitro. This is the first report demonstrating the anti-HBV effects of S. media, which is currently under early development as a potential anti-HBV drug candidate.
Keywords: anti-hepatitis B virus activity; Stellaria media (L.) Vill.; flavonoid; polysaccharide

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MDPI and ACS Style

Ma, L.; Song, J.; Shi, Y.; Wang, C.; Chen, B.; Xie, D.; Jia, X. Anti-Hepatitis B Virus Activity of Chickweed [Stellaria media (L.) Vill.] Extracts in HepG2.2.15 Cells. Molecules 2012, 17, 8633-8646.

AMA Style

Ma L, Song J, Shi Y, Wang C, Chen B, Xie D, Jia X. Anti-Hepatitis B Virus Activity of Chickweed [Stellaria media (L.) Vill.] Extracts in HepG2.2.15 Cells. Molecules. 2012; 17(7):8633-8646.

Chicago/Turabian Style

Ma, Lihua; Song, Jie; Shi, Yaqin; Wang, Changmei; Chen, Bin; Xie, Donghao; Jia, Xiaobin. 2012. "Anti-Hepatitis B Virus Activity of Chickweed [Stellaria media (L.) Vill.] Extracts in HepG2.2.15 Cells." Molecules 17, no. 7: 8633-8646.

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