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Metabolic Profiling of Lactococcus lactis Under Different Culture Conditions
Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
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Received: 25 May 2012; in revised form: 13 June 2012 / Accepted: 14 June 2012 / Published: 3 July 2012
Abstract: Gas chromatography mass spectrometry (GC-MS) and headspace gas chromatography mass spectrometry (HS/GC-MS) were used to study metabolites produced by Lactococcus lactis subsp. cremoris MG1363 grown at a temperature of 30 °C with and without agitation at 150 rpm, and at 37 °C without agitation. It was observed that L. lactis produced more organic acids under agitation. Primary alcohols, aldehydes, ketones and polyols were identified as the corresponding trimethylsilyl (TMS) derivatives, whereas amino acids and organic acids, including fatty acids, were detected through methyl chloroformate derivatization. HS analysis indicated that branched-chain methyl aldehydes, including 2-methylbutanal, 3-methylbutanal, and 2-methylpropanal are degdradation products of isoleucine, leucine or valine. Multivariate analysis (MVA) using partial least squares discriminant analysis (PLS-DA) revealed the major differences between treatments were due to changes of amino acids and fermentation products.
Keywords: Lactococcus lactis subsp. cremoris; metabolic profiling; GC-MS; derivatization
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Azizan, K.A.; Baharum, S.N.; Mohd Noor, N. Metabolic Profiling of Lactococcus lactis Under Different Culture Conditions. Molecules 2012, 17, 8022-8036.
Azizan KA, Baharum SN, Mohd Noor N. Metabolic Profiling of Lactococcus lactis Under Different Culture Conditions. Molecules. 2012; 17(7):8022-8036.
Azizan, Kamalrul Azlan; Baharum, Syarul Nataqain; Mohd Noor, Normah. 2012. "Metabolic Profiling of Lactococcus lactis Under Different Culture Conditions." Molecules 17, no. 7: 8022-8036.