Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis
AbstractThe objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. The protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a β-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.
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Filho, R.E.M.; Bandeira, S.P.; Brillhante, R.S.N.; Rocha, M.F.G.; Vasconcelos, I.M.; Pereira, M.L.; Castelo-Branco, D.S.C.M.; Rocha, F.A.C.; Camargo, Z.P.; Ramos, M.V.; Cordeiro, R.A.; Sidrim, J.J.C. Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis. Molecules 2012, 17, 7854-7863.
Filho REM, Bandeira SP, Brillhante RSN, Rocha MFG, Vasconcelos IM, Pereira ML, Castelo-Branco DSCM, Rocha FAC, Camargo ZP, Ramos MV, Cordeiro RA, Sidrim JJC. Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis. Molecules. 2012; 17(7):7854-7863.Chicago/Turabian Style
Filho, Renato Evando Moreira; Bandeira, Silviane Praciano; Brillhante, Raimunda Sâmia Nogueira; Rocha, Marcos Fábio Gadelha; Vasconcelos, Ilka Maria; Pereira, Mirella Leite; Castelo-Branco, Débora de Souza Collares Maia; Rocha, Francisco Airton Castro; Camargo, Zoilo Pires de; Ramos, Marcio Viana; Cordeiro, Rossana de Aguiar; Sidrim, José Júlio Costa. 2012. "Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis." Molecules 17, no. 7: 7854-7863.