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Molecules 2012, 17(7), 7854-7863; doi:10.3390/molecules17077854

Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis

1,* , 1, 1, 1,2, 3, 3, 1, 1, 4, 3, 1 and 1
1 Specialized Medical Mycology Center, School of Medicine, Federal University of Ceará, Fortaleza, CE, CEP, 60430-270, Brazil 2 Postgraduate Program in Veterinary Sciences, School of Veterinary Medicine, Ceará State University, Fortaleza, CE, CEP, 60740-000, Brazil 3 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Pici Campus, Fortaleza, CE, 60455-760, Brazil 4 Department of Microbiology, Immunology and Parasitology, São Paulo Federal University, São Paulo, 04021-001, Brazil
* Author to whom correspondence should be addressed.
Received: 23 March 2012 / Revised: 18 June 2012 / Accepted: 20 June 2012 / Published: 28 June 2012
(This article belongs to the Section Medicinal Chemistry)
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The objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. The protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a β-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.
Keywords: coccidioidomycosis; immunodiagnosis; antigens; β-glucosidase; glutamine coccidioidomycosis; immunodiagnosis; antigens; β-glucosidase; glutamine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Filho, R.E.M.; Bandeira, S.P.; Brillhante, R.S.N.; Rocha, M.F.G.; Vasconcelos, I.M.; Pereira, M.L.; Castelo-Branco, D.S.C.M.; Rocha, F.A.C.; Camargo, Z.P.; Ramos, M.V.; Cordeiro, R.A.; Sidrim, J.J.C. Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis. Molecules 2012, 17, 7854-7863.

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