Next Article in Journal
Self Assembled Films of Porphyrins with Amine Groups at Different Positions: Influence of Their Orientation on the Corrosion Inhibition and the Electrocatalytic Activity
Previous Article in Journal
Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2012, 17(7), 7810-7823; doi:10.3390/molecules17077810

Molecular Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene from Juglans regia

1
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China
2
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China
3
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
4
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, Hubei, China
*
Authors to whom correspondence should be addressed.
Received: 29 May 2012 / Revised: 11 June 2012 / Accepted: 11 June 2012 / Published: 26 June 2012
Download PDF [629 KB, uploaded 18 June 2014]

Abstract

Phenylalanine ammonia-lyase (PAL) is the first key enzyme of the phenypropanoid pathway. A full-length cDNA of PAL gene was isolated from Juglans regia for the first time, and designated as JrPAL. The full-length cDNA of the JrPAL gene contained a 1935bp open reading frame encoding a 645-amino-acid protein with a calculated molecular weight of about 70.4 kD and isoelectric point (pI) of 6.7. The deduced JrPAL protein showed high identities with other plant PALs. Molecular modeling of JrPAL showed that the 3D model of JrPAL was similar to that of PAL protein from Petroselinum crispum (PcPAL), implying that JrPAL may have similar functions with PcPAL. Phylogenetic tree analysis revealed that JrPAL shared the same evolutionary ancestor of other PALs and had a closer relationship with other angiosperm species. Transcription analysis revealed that JrPAL was expressed in all tested tissues including roots, stems, and leaves, with the highest transcription level being found in roots. Expression profiling analyses by real-time PCR revealed that JrPAL expression was induced by a variety of abiotic and biotic stresses, including UV-B, wounding, cold, abscisic acid and salicylic acid.
Keywords: phenylalanine ammonia-lyase (PAL); Juglans regia; molecular cloning; expression pattern phenylalanine ammonia-lyase (PAL); Juglans regia; molecular cloning; expression pattern
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Xu, F.; Deng, G.; Cheng, S.; Zhang, W.; Huang, X.; Li, L.; Cheng, H.; Rong, X.; Li, J. Molecular Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene from Juglans regia. Molecules 2012, 17, 7810-7823.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top