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Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation
State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Ministry of Education, No. 24 Tongjiaxiang, Nanjing 210009, China
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Received: 13 April 2012; in revised form: 13 June 2012 / Accepted: 21 June 2012 / Published: 26 June 2012
Abstract: A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb Dipsaci Radix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from Dipsaci Radix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from Dipsaci Radix.
Keywords: akebia saponin D; Dipsaci Radix; large-scale preparation; macroporous resin
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Cite This Article
MDPI and ACS Style
Wu, Y.; Ji, D.; Liu, Y.; Zhang, C.; Yang, Z. Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation. Molecules 2012, 17, 7798-7809.
Wu Y, Ji D, Liu Y, Zhang C, Yang Z. Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation. Molecules. 2012; 17(7):7798-7809.
Wu, Yue; Ji, De; Liu, Yunfei; Zhang, Chunfeng; Yang, Zhonglin. 2012. "Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation." Molecules 17, no. 7: 7798-7809.