Molecules 2012, 17(7), 7798-7809; doi:10.3390/molecules17077798
Article

Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation

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Received: 13 April 2012; in revised form: 13 June 2012 / Accepted: 21 June 2012 / Published: 26 June 2012
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb Dipsaci Radix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from Dipsaci Radix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from Dipsaci Radix.
Keywords: akebia saponin D; Dipsaci Radix; large-scale preparation; macroporous resin
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MDPI and ACS Style

Wu, Y.; Ji, D.; Liu, Y.; Zhang, C.; Yang, Z. Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation. Molecules 2012, 17, 7798-7809.

AMA Style

Wu Y, Ji D, Liu Y, Zhang C, Yang Z. Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation. Molecules. 2012; 17(7):7798-7809.

Chicago/Turabian Style

Wu, Yue; Ji, De; Liu, Yunfei; Zhang, Chunfeng; Yang, Zhonglin. 2012. "Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation." Molecules 17, no. 7: 7798-7809.

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