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Molecules 2012, 17(7), 7798-7809; doi:10.3390/molecules17077798
Article

Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation

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*  and *
State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Ministry of Education, No. 24 Tongjiaxiang, Nanjing 210009, China
* Authors to whom correspondence should be addressed.
Received: 13 April 2012 / Revised: 13 June 2012 / Accepted: 21 June 2012 / Published: 26 June 2012
(This article belongs to the Section Natural Products)
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Abstract

A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb Dipsaci Radix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from Dipsaci Radix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from Dipsaci Radix.
Keywords: akebia saponin D; Dipsaci Radix; large-scale preparation; macroporous resin akebia saponin D; Dipsaci Radix; large-scale preparation; macroporous resin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wu, Y.; Ji, D.; Liu, Y.; Zhang, C.; Yang, Z. Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation. Molecules 2012, 17, 7798-7809.

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