Isolation and Partial Characterization of an Antifungal Protein Produced by Bacillus licheniformis BS-3
Abstract
:1. Introduction
2. Results
2.1. Purification of the Antifungal Protein
Step | Amount of proteins (mg) | Total activity (AU) | Specific activity (AU/mg) | Recovery (%) | Purification (-fold) |
---|---|---|---|---|---|
Crude supernatant | 747.24 | 53,770 | 71.96 | 100.00 | 1.00 |
Ammonium sulfate precipitation | 186.86 | 50,482 | 270.16 | 93.89 | 3.75 |
DEAE cation exchange | 113.67 | 43,940 | 386.56 | 81.72 | 5.40 |
Gel filtration chromatography | 74.31 | 23,200 | 1352.85 | 43.15 | 18.80 |
2.2. Inhibitory Spectrum
2.3. Effects of Enzymes, Heat, and pH
2.4. Enzymatic Activities
Enzymes | Substrates | Concentration | Enzyme activity (U/mL) |
---|---|---|---|
Chitinase | Colloidal chitin | 1% | n.d. |
Cellulase | CM-cellulose | 1.25% | n.d. |
Xylanase | Xylan | 0.5% | n.d. |
Protease | Casein | 1% | 5,140 |
Lyozyme | M. lysodeikticus | Optical density of 1.5 | n.d. |
β- N-acetyl-glucosaminidase | p-Nitrophenyl-N-acetylglucosaminide | 5 mM | n.d. |
3. Discussion
4. Experimental
4.1. Antifungal Strain
4.2. Indicator Strains
4.3. Growth and Antifungal Protein Production
4.4. Quantification of Antifungal Activity
4.5. Extraction and Purification of Antifungal Protein
4.6. Electrophoresis and HPLC
4.7. Effects of Enzymes, Heat, and pH
4.8. Measurement of Enzyme Activities
5. Conclusions
Acknowledgements
- Sample Availability: Samples of the F2 protein are available from the authors.
References and notes
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Cui, T.-B.; Chai, H.-Y.; Jiang, L.-X. Isolation and Partial Characterization of an Antifungal Protein Produced by Bacillus licheniformis BS-3. Molecules 2012, 17, 7336-7347. https://doi.org/10.3390/molecules17067336
Cui T-B, Chai H-Y, Jiang L-X. Isolation and Partial Characterization of an Antifungal Protein Produced by Bacillus licheniformis BS-3. Molecules. 2012; 17(6):7336-7347. https://doi.org/10.3390/molecules17067336
Chicago/Turabian StyleCui, Tang-Bing, Hai-Yun Chai, and Li-Xiang Jiang. 2012. "Isolation and Partial Characterization of an Antifungal Protein Produced by Bacillus licheniformis BS-3" Molecules 17, no. 6: 7336-7347. https://doi.org/10.3390/molecules17067336