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Molecules 2012, 17(6), 6886-6900; doi:10.3390/molecules17066886
Article

Dot Immunobinding Assay Method with Chlorophyll Removal for the Detection of Southern Rice Black-Streaked Dwarf Virus

1, 1, 2, 1, 1, 1, 1, 1,*  and 1,*
Received: 23 April 2012; in revised form: 30 May 2012 / Accepted: 31 May 2012 / Published: 5 June 2012
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Abstract: Southern rice black-streaked dwarf virus (SRBSDV), a new virus from Fiji, has seriously damaged rice crops in southern China and northern Vietnam in recent years. This virus is difficult to diagnose in the early stages of infection, and is very destructive at the late stage. In the present study, a dot immunobinding assay (DIBA) that has a high sensitivity for diagnosing SRBSDV was developed. Two kinds of treatment for the DIBA were evaluated to determine the most effective one for removing chlorophyll interferences via rice extraction. The first included several reagents to remove chlorophyll, namely, the alkaline reagents like magnesium oxide and alumina oxide, the adsorbent reagents like activated carbon and bentonite, as well as the extraction agent acetone. The second and third treatments, which were used to remove chlorophyll in blot membrane-nitrocellulose and polyvinylidene fluoride (PVDF), included several organic solvents containing methanol, ethanol, acetone, ethyl acetate, and diethyl ether. The results showed that activated carbon and methanol yielded the best contrasting purple color for the infected samples by decreasing the chlorophyll content.
Keywords: Southern rice black-streaked dwarf virus (SRBSDV); dot immunobinding assay (DIBA); chlorophyll removal Southern rice black-streaked dwarf virus (SRBSDV); dot immunobinding assay (DIBA); chlorophyll removal
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, Z.; Liu, J.; Zeng, M.; Wang, Z.; Yu, D.; Yin, C.; Jin, L.; Yang, S.; Song, B. Dot Immunobinding Assay Method with Chlorophyll Removal for the Detection of Southern Rice Black-Streaked Dwarf Virus. Molecules 2012, 17, 6886-6900.

AMA Style

Chen Z, Liu J, Zeng M, Wang Z, Yu D, Yin C, Jin L, Yang S, Song B. Dot Immunobinding Assay Method with Chlorophyll Removal for the Detection of Southern Rice Black-Streaked Dwarf Virus. Molecules. 2012; 17(6):6886-6900.

Chicago/Turabian Style

Chen, Zhuo; Liu, Jiaju; Zeng, Mengjiao; Wang, Zhenchao; Yu, Dandan; Yin, Chengjun; Jin, Linhong; Yang, Song; Song, Baoan. 2012. "Dot Immunobinding Assay Method with Chlorophyll Removal for the Detection of Southern Rice Black-Streaked Dwarf Virus." Molecules 17, no. 6: 6886-6900.



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