Pristimerin, a Triterpenoid, Inhibits Tumor Angiogenesis by Targeting VEGFR2 Activation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Pristimerin Inhibits Tumor Growth and Tumor Angiogenesis in a Xenograft Mouse Model
2.2. Pristimerin Inhibits Angiogenesis in Vivo and VEGF-induced Vessel Sprouting ex Vivo
2.3. Pristimerin Inhibits the VEGF-Induced Chemotactic Motility, Capillary Structure Formation and Proliferation of HUVECs in Vitro
2.4. Influence of Pristimerin on VEGFR2 and Related Signaling Pathways
2.5. Discussion
3. Experimental
3.1. Cell lines and Cell Culture
3.2. Reagents and Antibodies
3.3. Human Breast Tumor Xenograft Mouse Model
3.4. Histology and Immunohistochemistry
3.5. Chicken Chorioallantoic Membrane (CAM) Assay
3.5. Rat Aortic Ring Assay
3.7. Cell Proliferation Assay by Bromodeoxyuridine (BrdU) Incorporation
3.8. Wound-Healing Migration Assay
3.9. Transwell Migration Assay
3.10. Capillary-Like Tube Formation Assay
3.11. Western Immunoblot Analysis
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
References and Notes
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Mu, X.; Shi, W.; Sun, L.; Li, H.; Jiang, Z.; Zhang, L. Pristimerin, a Triterpenoid, Inhibits Tumor Angiogenesis by Targeting VEGFR2 Activation. Molecules 2012, 17, 6854-6868. https://doi.org/10.3390/molecules17066854
Mu X, Shi W, Sun L, Li H, Jiang Z, Zhang L. Pristimerin, a Triterpenoid, Inhibits Tumor Angiogenesis by Targeting VEGFR2 Activation. Molecules. 2012; 17(6):6854-6868. https://doi.org/10.3390/molecules17066854
Chicago/Turabian StyleMu, Xianmin, Wei Shi, Lixin Sun, Han Li, Zhenzhou Jiang, and Luyong Zhang. 2012. "Pristimerin, a Triterpenoid, Inhibits Tumor Angiogenesis by Targeting VEGFR2 Activation" Molecules 17, no. 6: 6854-6868. https://doi.org/10.3390/molecules17066854