Next Article in Journal
The Role of Bioactive Compounds on the Promotion of Neurite Outgrowth
Previous Article in Journal / Special Issue
Calculation of the Stabilization Energies of Oxidatively Damaged Guanine Base Pairs with Guanine
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

1H-Nuclear Magnetic Resonance Analysis of the Triacylglyceride Composition of Cold-Pressed Oil from Camellia japonica

1
Areeiro Phytopathological Station, Pontevedra Deputation, Subida a la Robleda s/n., E36153 Pontevedra, Spain
2
Department of Organic Chemistry, Faculty of Science, University of Santiago de Compostela, E27002 Lugo, Spain
3
Department of Plant Production, University of Santiago de Compostela, E27002 Lugo, Spain
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(6), 6716-6727; https://doi.org/10.3390/molecules17066716
Submission received: 1 March 2012 / Revised: 23 May 2012 / Accepted: 28 May 2012 / Published: 4 June 2012
(This article belongs to the Special Issue ECSOC-15)

Abstract

:
Camellia japonica (CJ) has oil-rich seeds, but the study of these oils has received little attention and has mainly focused only on their health properties. In the present work the relative composition of the fatty acid (FA) components of the triglycerides in cold-pressed oil from CJ is studied by 1H-NMR. The results obtained were: 75.75%, 6.0%, 0.17% and 18.67%, for oleic, linoleic, linolenic and saturated FA respectively. Levels of C18 unsaturated FA found in CJ oil were similar to those reported for olive oils. We also checked the possibility of using 13C-NMR spectroscopy; however, the results confirmed the drawback of 13C over 1H-NMR for the study of FA components of CJ triglycerides due to its low gyromagnetic ratio and its very low natural abundance.

1. Introduction

The genus Camellia, comprising more than 200 species, includes evergreen shrubs and trees belonging to the Theaceae family, which are grown mainly for the preparation of tea with the leaves and buds, for the seeds in order to obtain oil, and as ornamental plants [1]. Although the Camellia is native to Asia [2], the cultivated species are well adapted in several other countries and possess great economic value. Among the camellia species, the economic value of the Camellia japonica (CJ) ranks the highest due to its beautiful ornamental flowers. Indeed, over 32,000 cultivars are registered [3]. In Galicia (NW Spain), one of the most important Camellia producing-regions in Europe, about 2.5 million Camellia plants (most of them CJ) are produced in nurseries for use as houseplants and in gardening each year and then exported to markets in Belgium, The Netherlands, France, the United Kingdom and Portugal.
CJ does not only have an ornamental value, as several parts of the plant, including leaves, bark, flowers, flower buds, twigs, seeds, the pericarp and roots, have been used traditionally in oriental ethnomedicine for health purposes, such as the treatment of stomach disorders, blood vomiting and bleeding due to internal and external injury, as well as a tonic and anti-inflammatory agent [4].
As chemical constituents of this natural medicine, several triterpenes, flavonoids and phenolic compounds were reported [5,6,7,8,9,10,11,12]. Moreover, when analyzing and studying the therapeutic properties of CJ, modern science has made it possible to specify the potential medical significance of their antimicrobial [13], antioxidant [14,15], antitumoral [16], neuronal cell protective [17], antihistaminic and anti-allergic [18,19,20], antiviral [21,22], hypoglycaemic potential [23], ethanol absorption inhibition [24] and skin healing properties [25].
Seeds of several species belonging to the Oleifera, Paracamellia, Camellia, and Furfuracea sections are used to extract high quality edible oil [26]. With a unique flavor and taste, good storage stability and claimed health benefits, Camellia oil, mainly obtained from wild plants of the species C. oleifera, C. semiserrata, and C. chekiangolomy, is often a target for adulteration or mislabeling in China because it is a high priced product with high nutritional and medicinal values [27,28]. Surprisingly, data on Camellia oil in the referred literature is scarce, and mainly focused on C. oleifera oil (tea seed oil), since in central and south China it is used extensively as a cooking oil.
Edible oils are mainly made up of triacylglycerols (TAG) which comprise more than 95 to 99% of the total lipids present. In TAG the HO- of the glycerol joins the -COOH of the fatty acid (FA) to form ester bonds. Because there are a large number of individual FAs, with different chain lengths, and degrees of unsaturation and position on the glycerol molecules, defining the TAG composition of an oil is a very challenging task [29]. In fact, each type of oil has a different TAG profile which determines the nature of its physicochemical and nutritional properties, and also provides information on the quality of the oil. In recent years, both industry and consumers have shown an increased interest in the latter.
Our literature survey revealed that several methods for the qualitative and quantitative determination of TAGs in oil samples are available [29,30,31]. These techniques include mainly gas-liquid chromatography, high performance chromatography in normal and reversed phase mode, thin-layer chromatography and supercritical fluid chromatography. However, these methods are labor-intensive and time consuming and also involve a complex series of chemical manipulation steps.
Nuclear magnetic resonance (NMR) has become one of the most promising methods to determine organic structures in complex matrices such as foods, and pharmaceutical and biological samples [32,33]. 1H nuclear magnetic resonance (1H-NMR) offers many advantages over alternative analytical methods to study edible oils because it allows the rapid, simultaneous, noninvasive, and nondestructive study of oil composition, and also provides information about the acyl distribution and the acyl positional distribution of TAGs [34,35,36,37].
Although Galicia is one of the most important Camellia japonica producing-regions in Europe, there is no information available on the composition of CJ oil from this region. This lack of knowledge commonly leads to the waste of this quite easily obtained product. Thus, most of the Galician producers are not able to maximize their profits. In this context, the characterization and standardization of CJ oil would allow for the increase of its use in industries, and as a consequence, the economic value of this natural resource. All of this would stimulate its production. At the same time, it is necessary to measure elemental composition in order to provide correct denominations that can establish the minimum marketing level of the product and provide adequate consumer protection. The aim of this work was to study the TAG composition of cold pressed oil obtained from C. japonica cultivated in NW Spain by 1H-NMR analysis.

2. Results and Discussion

The structure of the major TAGs present in CJ oil highlight the most singular kinds of hydrogen from the NMR point of view, and is represented in Figure 1. Vinylic hydrogens (Hv) have a characteristic chemical shift, and could be used to determine the ratio of saturated to unsaturated esters. Bisallylic hydrogens (Hd, Ht) could be used to differentiate the nature of the unsaturated components. Finally, the tertiary hydrogen in the glycerin moiety (Hg) could be used to quantify the ratio of saturated to unsaturated esters since there is only one hydrogen for each TAG molecule.
Figure 1. Chemical structure of the main triacylglycerols in oils.
Figure 1. Chemical structure of the main triacylglycerols in oils.
Molecules 17 06716 g001
The proton resonances of the TAG acyl chains were assigned according to the literature data [38,39] and are shown in Table 1 and Table 2.
Table 1. Assignment of the signals of Camellia japonica oil 1H-NMR spectra (300 MHz for 1H).
Table 1. Assignment of the signals of Camellia japonica oil 1H-NMR spectra (300 MHz for 1H).
SignalFunctional groupMultiplicityChemical shift (ppm)
1I (dd) –CH3dd0.96–0.82
2H (m) –CH2m1.43–1.16
3G (m) –CH2–C–CO2m1.70–1.51
4F (m) –CH2–CO2m2.11–1.91
5E (m) –C–CH2–C=C–m2.38–2.21
6D (t) –C=C–CH2–C=C––C=C–CH2–C=C–CH2–C=Ct2.83–2.73
7C (dd) –C–CH2–O–CO–Cdd4.21–4.08
8B (dd) –C–CH2–O–CO–Cdd4.36–4.22
9A (m) CH(–C–O–CO–C–)2+ C–HC=CH–Cm5.43–5.13
Signal multiplicity: s, single; d, doublet; t, triplet; m, multiplet. The signal number agrees with those in Figure 2.
Table 2. Assignment of the signals of Camellia japonica oil 1H-NMR spectra (750 MHz for 1H).
Table 2. Assignment of the signals of Camellia japonica oil 1H-NMR spectra (750 MHz for 1H).
SignalFunctional groupMultiplicityChemical shift (ppm)
1I (t) –CH3t0.89–0.86
2H (m) –CH2m1.35–1.23
3G (m) –CH2–C–CO2m1.64–1.57
4D (m) –CH2–CO2m2.02–1.98
5E (m) –CH2–CO2m2.06–2.02
6F (dt) –C–CH2–C=C–dt2.33–2.28
7C (t) –C=C–CH2–C=C–t2.78–2.74
8L (m) –C=C–CH2–C=C–CH2–C=Cm2.81–2.78
9A (dd) –C–CH2–O–CO–Cdd4.15–4.06
10B (dd) –C–CH2–O–CO–Cdd4.30–4.26
11K (m) CH(–C–O–CO–C–)2m5.27–5.24
12J (m) C–HC=CH–Cm5.37–5.30
Signal multiplicity: s, single; d, doublet; t, triplet; m, multiplet; dt, double of triplet; dd, doublet of doblet. The signal number agrees with those in Figure 3.
We studied the 300 MHz spectra for CJ oil (Figure 2); however the spectra obtained did not allow for the accurate integration of the tertiary hydrogen Hg of the glycerine moiety, and no difference was found between bisallylic hydrogens (δ = 2.80 ppm approx.).
The use of a 750 MHz spectrometer to analyze the samples rendered a higher resolution spectra that in turn allowed for the separated integration of the signal from the tertiary glycerin hydrogen, and the vinylic ones (Figure 3). For CJ oil, once the peak of Hg was normalized to 100, the signal of vinyl hydrogens was integrated as 5.26 (5.37–5.50 ppm), the bisallylic hydrogens giving 0.36 (linoleic) and 0.02 (linolenic).
Figure 2. Camellia japonica oil 1H-NMR spectra (300 MHz for 1H).
Figure 2. Camellia japonica oil 1H-NMR spectra (300 MHz for 1H).
Molecules 17 06716 g002
Figure 3. Camellia japonica oil 1H-NMR spectra (750 MHz for 1H).
Figure 3. Camellia japonica oil 1H-NMR spectra (750 MHz for 1H).
Molecules 17 06716 g003
The equations were defined as follows, with A, B, C and D as the fractions of each kind of FA involved in the TAG structure:
Molecules 17 06716 i001
The measured integrals [40] can be summarized as: Hv = 568.98, Hd = 39.88, Ht = 3.45 and Hg = 100. Thus the values of each kind of acid are: A 80.67%, B 6.65%, C 0.29% and D 12.39%, for oleic, linoleic, linolenic, and saturated fatty acids, respectively.
Table 3 shows the typical levels of C18 unsaturated and total saturated FA reported in literature for common oils. The values recorded for linoleic, linolenic and oleic FA were similar to those obtained for olive oils.
Table 3. Typical levels (in %) of C18 unsaturated fatty acids and total saturated fatty acids in common oils.
Table 3. Typical levels (in %) of C18 unsaturated fatty acids and total saturated fatty acids in common oils.
OilLinoleicLinolelicOleicSaturated
Virgin olive a6.00.781.312
Hazelnut a13.50.077.98.6
Peanut a21.60.061.816.6
Virgin olive b5.90.780.013.4
Olive b7.40.777.514.4
Hazelnut b10.70.081.08.3
Corn b51.00.733.015.3
Sunflower b58.80.029.212.0
Soybean b54.210.420.415.0
Linseed b17.154.220.08.7
Avocado c1016520
Tea seed oil c,*10<18010
Pumpkin c40<14010
Soybean c5072515
Canola c2010607
Olive c8<17514
Camellia japonica **6.650.2980.6712.39
a [41]; b [42]; c [43]; * Oil fromCamellia oleifera; ** Present work.
We also checked the possibility of using 13C-NMR spectroscopy for the rapid analysis of CJ oil [44]. Although this technique has been used for the study of the composition of several oils, its main drawback is the low natural abundance of the 13C isotope. The same sample used for the 1H-NMR experiment was used for the 13C acquisition (40 min, 1107 scans) and Figure 4 shows the spectrum obtained without any apodization. The carbonyl region showed four peaks (Figure 5 top): 173.42 corresponds to the carbonyl group in saturated fatty ester chains, 173.38 and 172.97 to the carbonyls in oleic esters, and 173.37 and a shoulder at 172.96 to linoleic ester chains.
The characteristic vinylic hydrogen region (Figure 5 bottom) showed peaks of oleic ester at 130.16, 130.14, 129.84, and 129.81, together with 130.35, 130.11, 120.20, and 128.02 from linoleic esters, but no peaks corresponding to linolenic could be detected. These results confirmed the well known handicap of 13C for rapid analysis of TAGs due to its low gyromagnetic ratio and its very low natural abundance.
Figure 4. Camellia japonica oil 13C-NMR spectra (189 MHz).
Figure 4. Camellia japonica oil 13C-NMR spectra (189 MHz).
Molecules 17 06716 g004
Figure 5. Camellia japonica oil 13C-NMR spectra (189 MHz) of carbonyl (top) and vynilic (bottom) regions.
Figure 5. Camellia japonica oil 13C-NMR spectra (189 MHz) of carbonyl (top) and vynilic (bottom) regions.
Molecules 17 06716 g005

3. Experimental

3.1. Oil Obtention

The extraction of Camellia japonica oil (CJO) essentially involved preconditioning of camellia fruits, mechanical pressing of seeds and filtering to remove oil impurities. Mature Camellia japonica fruits were obtained from healthy plants grown in the live germplasm camellia bank at the Estación Fitopatolóxica do Areeiro (Pontevedra, Galicia, Spain). The fruits are large apple-shaped woody capsules that can contain several seeds, which are dropped on to the ground in late summer. For the study, ripe fruits were collected as soon as the capsules began to split open, and allowed to dry at room temperature for a week. Then seeds were removed from capsules. After crushing, seeds were cold-pressed for oil. The oil often had suspended particles in it which had to be filtered out. Finally, the CJ oil obtained was stored in amber glass bottles at ambient temperature.

4.2. 1H-NMR Analysis

1H-NMR analyses were performed on Varian Mercury 300 (300 MHz for 1H) and Varian Inova 750 (750 MHz for 1H) instruments (Agilent Technologies®, Palo Alto, CA, USA), equipped with a 5 mm probe. Each oil sample, weighing 0.2 g, was dissolved in 400 µL of deuterated chloroform CDCl3, (Sigma-Aldrich®, Madrid, Spain) shaken in a vortex mixer, and the resulting mixture was placed into a 5-mm diameter ultra-precision NMR sample tubes (Norell®, Landisville, PA, USA). The temperature of the sample in the probe was 30 °C. The chemical shifts are reported in ppm, using the solvent proton signal as standard. The area of the signals was determined by using the equipment software, and the integrations were carried out three times to obtain average values. All figures of the 1H-NMR spectra and of the expanded 1H-NMR spectrum regions were plotted at a fixed value of absolute intensity to be valid for comparative purposes.

4.3. 13C-NMR Analysis

13C-NMR analysis was performed on a Varian Inova 750 spectrometer working at 189 MHz for 13C. The same samples subjected to 1H-NMR analyses were used for 13C-NMR analysis.

4. Conclusions

In summary, 750 MHz 1H-NMR spectroscopy has proven to be a useful tool for the direct analysis of the triacylglyceride composition of cold-pressed oil from the Camellia japonica.

Acknowledgements

XUNTA DE GALICIA for financial support: Grants INCITE09 262346PR and PGIDIT06RAG26103PR. X.F. would also like to thank the Xunta de Galicia (Isidro Parga Pondal Program for young researchers, Grant No. IPP-020). JoDee Anderson for her linguistic support.
  • Sample Availability: Samples of the Camellia japonica oil used are available from the authors.

References and notes

  1. Ming, T.L.; Bartholomew, B. Theaceae. In Flora of China; Wu, Z.Y., Raven, P.H., Hong, D.Y., Eds.; Science Press: Beijing, China, and Missouri Botanical Garden: St. Louis, MO, USA, 2007; Volume 12, pp. 366–478. [Google Scholar]
  2. Gao, J.Y.; Parks, C.R.; Du, Y.Q. Collected Species of the Genus Camellia-An Illustrated Outline; Zhejiang Scientific & Technology: Hangzhou, China, 2005. [Google Scholar]
  3. Savige, T.J. The International Camellia Register; The International Camellia Society: Wirlinga, NSW, Australia, 1993. [Google Scholar]
  4. Yoshikawa, M.; Morikawa, T.; Asao, Y.; Fujiwara, E.; Nakamura, S.; Matsuda, H. Medicinal Flowers. XV. The Structures of Noroleanane- and Oleanane-Type Triterpene Oligoglycosides with Gastroprotective and Platelet Aggregation Activities from Flower Buds of Camellia Japonica. Chem. Pharm. Bull. 2007, 55, 606–612. [Google Scholar]
  5. Han, L.; Hatano, T.; Yoshida, T.; Okuda, T. Tannins of Theaceous Plants. V. Camelliatannins F, G and H, Three New Tannins from Camellia Jjaponica L. Chem. Pharm. Bull. 1994, 42, 1399–1409. [Google Scholar]
  6. Saito, N.; Yokoi, M.; Yamaji, M.; Honda, T. Cyanidin 3-p-Coumaroylglucoside in Camellia Species and Cultivars. Phytochemistry 1987, 26, 2761–2762. [Google Scholar]
  7. Thao, N.T.P.; Hung, T.M.; Lee, M.K.; Kim, J.C.; Min, B.S.; Bae, K. Triterpenoids from Camellia Japonica and their Cytotoxic Activity. Chem. Pharm. Bull. 2010, 58, 121–124. [Google Scholar] [CrossRef]
  8. Yoshikawa, M.; Morikawa, T.; Fujiwara, E.; Ohgushi, T.; Asao, Y.; Matsuda, H. New Noroleanane-Type Triterpene Saponins with Gastroprotective Effect and Platelet Aggregation Activity from the Flowers of Camellia Japonica: Revised Structures of Camellenodiol and Camelledionol. Heterocycles 2001, 55, 1653–1657. [Google Scholar] [CrossRef]
  9. Hatano, T.; Han, L.; Taniguchi, S.; Shingu, T.; Okuda, T.; Yoshida, T. Tannins and Related Polyphenols of Theaceous Plants. VIII. Camelliatannins C and E, New Complex Tannins from Camellia Japonica Leaves. Chem. Pharm. Bull. 1995, 43, 1629–1633. [Google Scholar]
  10. Azuma, C.M.; dos Santos, F.C.S.; Lago, J.H.G. Flavonoids and Fatty Acids of Camellia Japonica Leaves Extract. Braz. J. Pharamacogn. 2011, 21, 1159–1162. [Google Scholar]
  11. Cho, J.; Ji, S.; Moon, J.; Lee, K.; Jung, K.; Park, K. A Novel Benzoyl Glucoside and Phenolic Compounds from the Leaves of Camellia Japonica. Food Sci. Biotechnol. 2008, 17, 1060–1065. [Google Scholar]
  12. Zhang, Y.; Yin, C.; Kong, L.; Jiang, D. Extraction Optimisation, Purification and Major Antioxidant Component of Red Pigments Extracted from Camellia Japonica. Food Chem. 2011, 129, 660–664. [Google Scholar] [CrossRef]
  13. Kim, K.Y.; Davidson, P.M.; Chung, H.J. Antibacterial Activity in Extracts of Camellia Japonica L. Petals and its Application to a Model Food System. J. Food Protect. 2001, 64, 1255–1260. [Google Scholar]
  14. Onodera, K.; Hanashiro, K.; Yasumoto, T. Camellianoside, a Novel Antioxidant Glycoside from the Leaves of Camellia Japonica. Biosci. Biotechnol. Biochem. 2006, 70, 1995–1998. [Google Scholar] [CrossRef]
  15. Piao, M.J.; Yoo, E.S.; Koh, Y.S.; Kang, H.K.; Kim, J.; Kim, Y.J.; Kang, H.H.; Hyun, J.W. Antioxidant Effects of the Ethanol Extract from Flower of Camellia japonica via Scavenging of Reactive Oxygen Species and Induction of Antioxidant Enzymes. Int. J. Mol. Sci. 2011, 12, 2618–2630. [Google Scholar] [CrossRef]
  16. Thao, N.T.P.; Hung, T.M.; Lee, M.K.; Kim, J.C.; Min, B.S.; Bae, K. Triterpenoids from Camellia Japonica and their Cytotoxic Activity. Chem. Pharm. Bull. 2010, 58, 121–124. [Google Scholar] [CrossRef]
  17. Jeong, C.; Kim, J.H.; Choi, G.N.; Kwak, J.H.; Kim, D.; Heo, H.J. Protective Effects of Extract with Phenolics from Camellia (Camellia Japonica) Leaf Against Oxidative Stress-Induced Neurotoxicity. Food Sci. Biotechnol. 2010, 19, 1347–1353. [Google Scholar] [CrossRef]
  18. Onodera, K.I.; Tsuha, K.; Yasumoto-Hirose, M.; Tsuha, K.; Hanashiro, K.; Naoki, H.; Yasumoto, T. Okicamelliaside, an Extraordinarily Potent Anti-Degranulation Glucoside Isolated from Leaves of Camellia Japonica. Biosci. Biotechnol. Biochem. 2010, 74, 2532–2534. [Google Scholar] [CrossRef]
  19. Kuba, M.; Tsuha, K.; Tsuha, K.; Matsuzaki, G.; Yasumoto, T. In Vivo Analysis of the Anti-Allergic Activities of Camellia Japonica Extract and Okicamelliaside, a Degranulation Inhibitor. J. Health Sci. 2008, 54, 584–588. [Google Scholar] [CrossRef]
  20. Lee, J.; Kim, J.; Ko, N.; Mun, S.; Kim, D.; Kim, J.; Kim, H.; Lee, K.; Kim, Y.; Radinger, M.; et al. Camellia Japonica Suppresses Immunoglobulin E-Mediated Allergic Response by the Inhibition of Syk Kinase Activation in Mast Cells. Clin. Exp. Allergy 2008, 38, 794–804. [Google Scholar] [CrossRef]
  21. Park, J.C.; Hur, J.M.; Park, J.G.; Hatano, T.; Yoshida, T.; Miyashiro, H.; Min, B.S.; Hattori, M. Inhibitory Effects of Korean Medicinal Plants and Camelliatannin H from Camellia Japonica on Human Immunodeficiency Virus Type 1 Protease. Phytother. Res. 2002, 16, 422–426. [Google Scholar] [CrossRef]
  22. Akihisa, T.; Tokuda, H.; Ukiya, M.; Suzuki, T.; Enjo, F.; Koike, K.; Nikaido, T.; Nishino, H. 3-Epicabraleahydroxylactone and Other Triterpenoids from Camellia Oil and their Inhibitory Effects on Epstein-Barr Virus Activation. Chem. Pharm. Bull. 2004, 52, 153–156. [Google Scholar] [CrossRef]
  23. Somasundaram, A. Antidiabetic Activity of Methanolic Extract of Camellia japonica Leaves against Alloxan Induced Diabetes in Rats. Int. J. Pharm. BioSci. 2011, 1, 43–46. [Google Scholar]
  24. Yoshikawa, M.; Harada, E.; Murakami, T.; Matsuda, H.; Yamahara, J.; Murakami, N. Camelliasaponins B1, B2, C1 and C2, New Type Inhibitors of Ethanol Absorption in Rats from the Seeds of Camellia Japonica. Chem. Pharm. Bull. 1994, 42, 742–744. [Google Scholar] [CrossRef]
  25. Jung, E.; Lee, J.; Baek, J.; Jung, K.; Lee, J.; Huh, S.; Kim, S.; Koh, J.; Park, D. Effect of Camellia Japonica Oil on Human Type I Procollagen Production and Skin Barrier Function. J. Ethnopharmacol. 2007, 112, 127–131. [Google Scholar] [CrossRef]
  26. Ming, T.L. Monograph of the Genus Camellia; Yunnan Science and Technology Press: Kunming, China, 2000. [Google Scholar]
  27. Mondal, T.K. Camellia. In Wild Crop Relatives: Genomic and Breeding Resources; Kole, C., Ed.; Springer-Verlag: Berlin/Heidelberg, Germany, 2011; pp. 15–39. [Google Scholar]
  28. Wang, L.; Lee, F.S.C.; Wang, X.; He, Y. Feasibility Study of Quantifying and Discriminating Soybean Oil Adulteration in Camellia Oils by Attenuated Total Reflectance MIR and Fiber Optic Diffuse Reflectance NIR. Food Chem. 2006, 95, 529–536. [Google Scholar] [CrossRef]
  29. Buchgraber, M.; Ulberth, F.; Emons, H.; Anklam, E. Triacylglycerol Profiling by Using Chromatographic Techniques. Eur. J. Lipid Sci. Technol. 2004, 106, 621–648. [Google Scholar] [CrossRef]
  30. Laakso, P. Analysis of Triacylglycerols-Approaching the Molecular Composition of Natural Mixtures. Food Rev. Int. 1996, 12, 199–250. [Google Scholar] [CrossRef]
  31. Ribeiro, A.P.B.; Basso, R.C.; Grimaldi, R.; Gioielli, L.A.; Guaraldo, L.A. Instrumental Methods for the Evaluation of Interesterified Fats. Food Anal. Methods 2009, 2, 282–302. [Google Scholar] [CrossRef]
  32. Martinez, I.; Aursand, M.; Erikson, U.; Singstad, T.E.; Veliyulin, E.; Van Der Zwaag, C. Destructive and Non-Destructive Analytical Techniques for Authentication and Composition Analyses of Foodstuffs. Trends Food Sci. Technol. 2003, 14, 489–498. [Google Scholar] [CrossRef]
  33. Beyer, T.; Diehl, B.; Holzgrabe, U. Quantitative NMR Spectroscopy of Biologically Active Substances and Excipients. Bioanal. Rev. 2010, 2, 1–22. [Google Scholar]
  34. Guillén, M.D.; Ruiz, A. High Resolution 1H Nuclear Magnetic Resonance in the Study of Edible Oils and Fats. Trends Food Sci. Technol. 2001, 12, 328–338. [Google Scholar] [CrossRef]
  35. Guillén, M.D.; Ruiz, A. 1H nuclear magnetic resonance as a fast tool for determining the composition of acyl chains in acylglycerol mixtures. Eur. J. Lipid Sci. Technol. 2003, 105, 502–507. [Google Scholar] [CrossRef]
  36. Guillen, M.D.; Ruiz, A. Rapid simultaneous determination by proton NMR of unsaturation and composition of acyl groups in vegetable oils. Eur. J. Lipid Sci. Technol. 2003, 105, 688–696. [Google Scholar] [CrossRef]
  37. Lie Ken Jie, M.S.F.; Mustafa, J. High-Resolution Nuclear Magnetic Resonance Spectroscopy-Applications to Fatty Acids and Triacylglycerols. Lipids 1997, 32, 1019–1034. [Google Scholar] [CrossRef]
  38. Vlahov, G. Application of NMR to the Study of Olive Oils. Prog. Nucl. Magn. Reson. Spectrosc. 1999, 35, 341–357. [Google Scholar]
  39. Mannina, L.; Luchinat, C.; Patumi, M.; Emanuele, M.C.; Rossi, E.; Segre, A.L. Concentration Dependence of 13C-NMR Spectra of Triglycerides: Implications for the NMR Analysis of Olive Oil. Magn. Reson. Chem. 2000, 38, 886–890. [Google Scholar] [CrossRef]
  40. The acquisition of the data was carried out with a relaxation delay of 14 s and a pulse of 20° which ensures quantitative integrals for al protons with T1 < 3s.
  41. Guillén, M.D.; Ruiz, A. Study by Means of Proton Nuclear Magnetic Resonance of the Thermal Oxidation Process Undergone by Oils Rich in Oleic Acyl Groups. J. Am. Oil Chem. Soc. 2005, 82, 349–355. [Google Scholar] [CrossRef]
  42. Guillén, M.D.; Ruiz, A. Monitoring of Heat-Induced Degradation of Edible Oils by Proton NMR. Eur. J. Lipid Sci. Technol. 2008, 110, 52–60. [Google Scholar] [CrossRef]
  43. Haiyan, Z.; Bedgood, D.R.; Bishop, A.G.; Prenzler, P.D.; Robards, K. Endogenous biophenol, fatty acid and volatile profiles of selected oils. Food Chem. 2006, 100, 1544–1551. [Google Scholar]
  44. 13C-NMR (189 MHz, CDCl3) δ: 173.42, 173.38, 173.37, 172.97, 172.96, 130.35, 130.16, 130.14, 130.11, 129.84, 129.81, 128.20, 128.02, 69.02, 62.24, 34.34, 34.20, 34.17, 32.05, 29.91, 29.85, 29.81, 29.77, 29.68, 29.47, 29.35, 29.33, 29.26, 29.24, 29.20, 27.37, 27.32, 25.77, 25.03, 24.99, 22.83, 20.75, 14.26 ppm.

Share and Cite

MDPI and ACS Style

Salinero, C.; Feás, X.; Mansilla, J.P.; Seijas, J.A.; Vázquez-Tato, M.P.; Vela, P.; Sainz, M.J. 1H-Nuclear Magnetic Resonance Analysis of the Triacylglyceride Composition of Cold-Pressed Oil from Camellia japonica. Molecules 2012, 17, 6716-6727. https://doi.org/10.3390/molecules17066716

AMA Style

Salinero C, Feás X, Mansilla JP, Seijas JA, Vázquez-Tato MP, Vela P, Sainz MJ. 1H-Nuclear Magnetic Resonance Analysis of the Triacylglyceride Composition of Cold-Pressed Oil from Camellia japonica. Molecules. 2012; 17(6):6716-6727. https://doi.org/10.3390/molecules17066716

Chicago/Turabian Style

Salinero, Carmen, Xesús Feás, J. Pedro Mansilla, Julio A. Seijas, M. Pilar Vázquez-Tato, Pilar Vela, and María J. Sainz. 2012. "1H-Nuclear Magnetic Resonance Analysis of the Triacylglyceride Composition of Cold-Pressed Oil from Camellia japonica" Molecules 17, no. 6: 6716-6727. https://doi.org/10.3390/molecules17066716

Article Metrics

Back to TopTop