Simple, Rapid and Reliable Preparation of [11C]-(+)-a-DTBZ of High Quality for Routine Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Production
2.2. Purification with Sep-Pak C-18
Sep-Pak | Elution yields (%) | Break out (%) |
---|---|---|
C-18 | 22.1 ± 7.8 | 0.5 ± 0.4 |
tC-18 | 55.6 ± 10.2 | 1.2 ± 0.6 |
Vac tC-18 | 95.3 ± 2.6 | 2.5 ± 1.4 |
2.3. Quality Control
2.4. Biodistribution of [11C]-(+)-α-DTBZ in Normal Mice
0 min | 10 min | 20 min | 30 min | 60 min | |
---|---|---|---|---|---|
Blood | 12.10 ± 1.85 | 1.83 ± 0.19 | 1.93 ± 0.30 | 1.13 ± 0.07 | 1.33 ± 0.06 |
Heart | 20.87 ± 2.40 | 3.81 ± 0.69 | 2.56 ± 0.66 | 1.82 ± 0.12 | 1.43 ± 0.08 |
Liver | 2.85 ± 0.20 | 10.27 ± 1.47 | 13.16 ± 2.26 | 9.50 ± 0.99 | 10.61 ± 1.69 |
Spleen | 1.79 ± 0.51 | 5.36 ± 0.59 | 5.35 ± 0.69 | 3.19 ± 0.15 | 2.57 ± 0.28 |
Lung | 27.24 ± 9.56 | 5.03 ± 0.63 | 5.70 ± 0.30 | 3.34 ± 0.33 | 1.85 ± 0.44 |
Kidney | 3.32 ± 1.30 | 7.57 ± 0.87 | 7.62 ± 1.12 | 4.70 ± 0.04 | 3.42 ± 0.19 |
Striatum | 19.17 ± 1.25 | 13.00 ± 0.29 | 10.85 ± 1.56 | 5.75 ± 0.39 | 2.32 ± 0.16 |
Cortex | 16.29 ± 1.97 | 3.42 ± 0.08 | 2.76 ± 0.44 | 1.71 ± 0.10 | 0.93 ± 0.11 |
Striatum/cortex | 1.2 | 3.8 | 3.9 | 3.4 | 2.5 |
3. Experimental
3.1. Materials
3.2. Synthesis of 11C-(+)-α-DTBZ
3.3. Fully Automated Radiosynthesis
3.4. Instruments
3.5. Production of [11C]-Methyl Iodide and [11C]-Methyl-Triflate [11,12]
3.6. Automated On-Line Synthesis of [11C]-(+)-α-DTBZ
3.7. Quality Control
3.8. Biodistribution of [11C]-(+)-α-DTBZ in Normal Mice
4. Conclusions
Acknowledgment
References
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Zhang, J.; Zhang, X.; Li, Y.; Tian, J. Simple, Rapid and Reliable Preparation of [11C]-(+)-a-DTBZ of High Quality for Routine Applications. Molecules 2012, 17, 6697-6704. https://doi.org/10.3390/molecules17066697
Zhang J, Zhang X, Li Y, Tian J. Simple, Rapid and Reliable Preparation of [11C]-(+)-a-DTBZ of High Quality for Routine Applications. Molecules. 2012; 17(6):6697-6704. https://doi.org/10.3390/molecules17066697
Chicago/Turabian StyleZhang, Jinming, Xiaojun Zhang, Yungang Li, and Jiahe Tian. 2012. "Simple, Rapid and Reliable Preparation of [11C]-(+)-a-DTBZ of High Quality for Routine Applications" Molecules 17, no. 6: 6697-6704. https://doi.org/10.3390/molecules17066697