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Molecules 2012, 17(6), 6557-6568; doi:10.3390/molecules17066557

Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide

1,2,†, 2,†, 3, 4, 2, 5, 2, 6 and 2,7,*
1 School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China 2 Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China 3 School of Life Sciences, Hubei University, Wuhan 430062, China 4 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China 5 Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China 6 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China 7 School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China These authors contributed equally to the work.
* Author to whom correspondence should be addressed.
Received: 23 March 2012 / Revised: 9 May 2012 / Accepted: 22 May 2012 / Published: 30 May 2012
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Radix Glycyrrhizae polysaccharide (GP), the most important component of Radix Glycyrrhizae, has been reported to have many immunopharmacological activities. However, the mechanism by which GP affects dendritic cells (DCs) has not been elucidated. In this study, we investigated the effect of GP on murine bone marrow-derived DCs and the potential pathway through which GP exerts this effect. Mononuclear cells (MNCs) were isolated from murine bone marrow and induced to become DCs by culturing with GM-CSF and IL-4. Six days later, DCs were divided into three groups: control group, GP group and LPS group. After 48 h of treatment, phenotypic figures and antigen uptake ability were determined by FACS analysis. The proliferation of DC-stimulated allogenic CD3+ T cells was detected by WST-1. IL-12 p70 and IFN-γ, which are secreted by DCs and CD3+ T cells respectively, were quantified by ELISA. Additionally, IL-12 p40 mRNA expression was determined by real-time PCR. Alterations in TLR4-related signaling pathways were examined by performing an antibody neutralization experiment. Treatment of DCs with GP resulted in the enhanced expression of the cell surface molecules CD80, CD86 and MHC I-A/I-E. GP also increased the production of IL-12 p70 by DCs in a time-dependent manner. The endocytosis of FITC-dextran by DCs was suppressed by GP administration. Furthermore, GP-treated DCs enhanced both the proliferation and IFN-γ secretion of allogenic CD3+ T cells. Finally, the effects of GP on DCs were partially reduced by using inhibitors of TLR4, NF-κB, p38 MAPK or JNK. In conclusion, GP can induce the maturation of DCs, and does so, in part, by regulating a TLR4-related signaling pathway.
Keywords: Radix Glycyrrhizae polysaccharide; dendritic cells; maturation; immune regulation Radix Glycyrrhizae polysaccharide; dendritic cells; maturation; immune regulation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Li, X.; He, X.; Liu, B.; Xu, L.; Lu, C.; Zhao, H.; Niu, X.; Chen, S.; Lu, A. Maturation of Murine Bone Marrow-Derived Dendritic Cells Induced by Radix Glycyrrhizae Polysaccharide. Molecules 2012, 17, 6557-6568.

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