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Molecules 2012, 17(5), 4904-4914; doi:10.3390/molecules17054904
Article

Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates

1,2
,
2,3
,
2,4
,
2
 and
2,*
1 Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil 2 Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Boa Vista 50050-590 Recife, PE, Brazil 3 Post-graduation Program of Chemical Engineering, State Campinas University, Campinas 13083-852, São Paulo, Brazil 4 Post-graduation Program of Biological Sciences, Federal University of Pernambuco, Recife 50670-420 Pernambuco, Brazil
* Author to whom correspondence should be addressed.
Received: 9 February 2012 / Revised: 16 April 2012 / Accepted: 26 April 2012 / Published: 27 April 2012
(This article belongs to the Section Natural Products)
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Abstract

This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (IC), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
Keywords: Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Cardoso, A.; Lins, C.I.M.; dos Santos, E.R.; Silva, M.C.F.; Campos-Takaki, G.M. Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates. Molecules 2012, 17, 4904-4914.

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