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Molecules 2012, 17(5), 4904-4914; doi:10.3390/molecules17054904
Article

Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates

1,2
, 2,3
, 2,4
, 2
 and 2,*
1 Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil 2 Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Boa Vista 50050-590 Recife, PE, Brazil 3 Post-graduation Program of Chemical Engineering, State Campinas University, Campinas 13083-852, São Paulo, Brazil 4 Post-graduation Program of Biological Sciences, Federal University of Pernambuco, Recife 50670-420 Pernambuco, Brazil
* Author to whom correspondence should be addressed.
Received: 9 February 2012 / Revised: 16 April 2012 / Accepted: 26 April 2012 / Published: 27 April 2012
(This article belongs to the Section Metabolites)
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Abstract

This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (IC), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
Keywords: Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cardoso, A.; Lins, C.I.M.; dos Santos, E.R.; Silva, M.C.F.; Campos-Takaki, G.M. Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates. Molecules 2012, 17, 4904-4914.

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