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Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates
Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil
Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Boa Vista 50050-590 Recife, PE, Brazil
Post-graduation Program of Chemical Engineering, State Campinas University, Campinas 13083-852, São Paulo, Brazil
Post-graduation Program of Biological Sciences, Federal University of Pernambuco, Recife 50670-420 Pernambuco, Brazil
* Author to whom correspondence should be addressed.
Received: 9 February 2012; in revised form: 16 April 2012 / Accepted: 26 April 2012 / Published: 27 April 2012
Abstract: This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (IC), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
Keywords: Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates
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MDPI and ACS Style
Cardoso, A.; Lins, C.I.M.; dos Santos, E.R.; Silva, M.C.F.; Campos-Takaki, G.M. Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates. Molecules 2012, 17, 4904-4914.
Cardoso A, Lins CIM, dos Santos ER, Silva MCF, Campos-Takaki GM. Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates. Molecules. 2012; 17(5):4904-4914.
Cardoso, Antonio; Lins, Clarissa Izabel M.; dos Santos, Ednaldo Ramos; Silva, Marta C. Freitas; Campos-Takaki, Galba M. 2012. "Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates." Molecules 17, no. 5: 4904-4914.