Next Article in Journal
Suffruyabiosides A and B, Two New Monoterpene Diglycosides from Moutan Cortex
Previous Article in Journal
Gossypol Exhibits a Strong Influence Towards UDP-Glucuronosyltransferase (UGT) 1A1, 1A9 and 2B7-Mediated Metabolism of Xenobiotics and Endogenous Substances
Molecules 2012, 17(5), 4904-4914; doi:10.3390/molecules17054904

Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates

1 Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil 2 Nucleus of Research in Environmental Sciences (NPCIAMB), Catholic University of Pernambuco, Boa Vista 50050-590 Recife, PE, Brazil 3 Post-graduation Program of Chemical Engineering, State Campinas University, Campinas 13083-852, São Paulo, Brazil 4 Post-graduation Program of Biological Sciences, Federal University of Pernambuco, Recife 50670-420 Pernambuco, Brazil
* Author to whom correspondence should be addressed.
Received: 9 February 2012 / Revised: 16 April 2012 / Accepted: 26 April 2012 / Published: 27 April 2012
(This article belongs to the Section Natural Products)
Download PDF [530 KB, uploaded 18 June 2014]


This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (IC), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
Keywords: Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates Rhizopus arrhizus; chitosan; biopolymer; agroindustrial substrates
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Cardoso, A.; Lins, C.I.M.; dos Santos, E.R.; Silva, M.C.F.; Campos-Takaki, G.M. Microbial Enhance of Chitosan Production by Rhizopus arrhizus Using Agroindustrial Substrates. Molecules 2012, 17, 4904-4914.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert