Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases
Abstract
:1. Introduction
2. Results
2.1. Effect of Essential oil on KB Cell Proliferation
2.2. Effect of Essential Oil on Cell Cycle Phase Distribution in KB Cells
2.3. Effect of Essential Oil on Determination of Morphological Changes in KB Cells
2.4. Effect of Essential Oil on DNA Fragmentation in KB Cells
2.5. Essential Oil-Mediated Apoptosis of KB Cells Involves a Caspase-Dependent Mechanism
2.6. Mitochondrial Stress is an Important Event in the Essential Oil-Mediated Apoptosis of KB Cells
3. Discussion
4. Experimental
4.1. Plant Material and Isolation of the Essential Oil
4.2. Cell Culture
4.3. Measurement of Cell Viability
4.4. Cell Cycle Analysis
4.5. Nuclear Morphology Analysis
4.6. DNA Extraction and DNA Gel Electrophoresis
4.7. Preparation of Cytosolic and Mitochondrial Extract
4.8. Western Blot Analysis
4.9. Statistical Analysis
5. Conclusions
Conflict of interest
Acknowledgements
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Cha, J.-D.; Kim, J.-Y. Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases. Molecules 2012, 17, 3890-3901. https://doi.org/10.3390/molecules17043890
Cha J-D, Kim J-Y. Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases. Molecules. 2012; 17(4):3890-3901. https://doi.org/10.3390/molecules17043890
Chicago/Turabian StyleCha, Jeong-Dan, and Ji-Young Kim. 2012. "Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases" Molecules 17, no. 4: 3890-3901. https://doi.org/10.3390/molecules17043890