Molecules 2012, 17(3), 2714-2724; doi:10.3390/molecules17032714
Article

Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites

Lefki-Maria Papaspyridi 1 email, Nektarios Aligiannis 2 email, Evangelos Topakas 1 email, Paul Christakopoulos 1 email, Alexandros-Leandros Skaltsounis 2 email and Nikolas Fokialakis 2,* email
1 BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, GR-15700 Athens, Greece 2 Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Panepistimioupolis, GR-15771 Athens, Greece
* Author to whom correspondence should be addressed.
Received: 23 December 2011; in revised form: 20 February 2012 / Accepted: 22 February 2012 / Published: 6 March 2012
(This article belongs to the Section Natural Products)
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Abstract: The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus. The biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. The dichloromethane and methanol extract were fractioned by different techniques including Adsorption Chromatography and Fast Centrifugal Partition Chromatography (FCPC). The structures of pure compounds were elucidated with 1D/2D NMR-spectroscopic analyses, and chemical correlations combined with GC/MS and LC/MS experiments. Nineteen metabolites (e.g., fatty acids, phenolic metabolites, nucleotides and alkaloids) were isolated. Beyond the production of known metabolites, we report herein the production also of trans-3,4-dihydro-3,4,8-trihydroxynapthalen-1(2H)-one, indolo-3-carboxylic acid, 3-formylpyrrole and 4-hydroxybenzoic acid, that have pharmaceutical interest and are isolated for the first time from Pleurotus strains.This work indicates the great potential of the established bioprocess for the production of P. ostreatus mycelia with enhanced metabolic profile.
Keywords: Pleurotus ostreatus; submerged fermentation; stirred tank bioreactor; bioactive metabolites; fast centrifugal partition chromatography

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MDPI and ACS Style

Papaspyridi, L.-M.; Aligiannis, N.; Topakas, E.; Christakopoulos, P.; Skaltsounis, A.-L.; Fokialakis, N. Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites. Molecules 2012, 17, 2714-2724.

AMA Style

Papaspyridi L-M, Aligiannis N, Topakas E, Christakopoulos P, Skaltsounis A-L, Fokialakis N. Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites. Molecules. 2012; 17(3):2714-2724.

Chicago/Turabian Style

Papaspyridi, Lefki-Maria; Aligiannis, Nektarios; Topakas, Evangelos; Christakopoulos, Paul; Skaltsounis, Alexandros-Leandros; Fokialakis, Nikolas. 2012. "Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites." Molecules 17, no. 3: 2714-2724.

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