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Molecules 2012, 17(3), 2725-2737; doi:10.3390/molecules17032725
Article

Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation

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Received: 13 December 2011; in revised form: 24 February 2012 / Accepted: 28 February 2012 / Published: 6 March 2012
(This article belongs to the Section Metabolites)
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Abstract: Menispermum dauricum rhizome has been widely used in China to treat various cardiovascular and thrombosis disorders. Some studies have reported that the phenolic alkaloids of Menispermum dauricum rhizome (PAM) have protective effects against brain ischemia injury, but the mechanism of this action remains to be clarified. In the present study, we investigated the possible mechanisms of action of PAM on experimental brain ischemia injury. Oxygen and glucose deprivation (OGD) in rat primary cortical cultures and middle cerebral artery occlusion in rats were used to mimic ischemia-reperfusion injury, respectively. The results suggested that PAM protected rat primary cortical cultures against OGD-reoxygenation induced cytotoxicity. PAM decreased extracellular glutamate content and markedly prevented the effects induced by OGD on protein level of GLT-1 and EAAC1 glutamate transporters. In addition, it reduced intracellular ROS generation. In vivo, PAM significantly reduced cerebral infarct area and ameliorated neurological functional deficits at different time points. Our findings revealed that the possible mechanism of action of PAM protected against brain ischemia injury involves regulation of GLT-1, EAAC1 and ROS generation.
Keywords: Menispermum dauricum; phenolic alkaloids; brain ischemia-reperfusion; glutamate transporter; reactive oxygen species Menispermum dauricum; phenolic alkaloids; brain ischemia-reperfusion; glutamate transporter; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhao, B.; Chen, Y.; Sun, X.; Zhou, M.; Ding, J.; Zhan, J.-J.; Guo, L.-J. Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation. Molecules 2012, 17, 2725-2737.

AMA Style

Zhao B, Chen Y, Sun X, Zhou M, Ding J, Zhan J-J, Guo L-J. Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation. Molecules. 2012; 17(3):2725-2737.

Chicago/Turabian Style

Zhao, Bo; Chen, Yang; Sun, Xi; Zhou, Mei; Ding, Jie; Zhan, Jin-Jin; Guo, Lian-Jun. 2012. "Phenolic Alkaloids from Menispermum dauricum Rhizome Protect against Brain Ischemia Injury via Regulation of GLT-1, EAAC1 and ROS Generation." Molecules 17, no. 3: 2725-2737.


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