Next Article in Journal
Bioactive Phenolic Amides from Celtis africana
Previous Article in Journal
A Microwave-Assisted Bismuth Nitrate-Catalyzed Unique Route Toward 1,4-Dihydropyridines
Molecules 2012, 17(3), 2663-2674; doi:10.3390/molecules17032663
Article

Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications

1
, 1
, 2
, 1
, 3
, 1
, 1
, 1
, 4
 and 5,*
Received: 31 January 2012; in revised form: 22 February 2012 / Accepted: 27 February 2012 / Published: 5 March 2012
Download PDF [376 KB, uploaded 18 June 2014]
Abstract: : A rapid LC-MS/MS method with good accuracy and sensitivity was developed and validated for the pharmacokinetics study of metoprolol (MP) in beagle dogs. The plasma samples were simply precipitated by methanol and then analyzed by LC-MS/MS. An Ultimate XB-C18 column (150 × 2.1 mm ID, 5 μm) was used for separation, with methanol-water containing 0.2% formic acid (65:35, v/v) as the mobile phase at a flow rate of 0.2 mL/min. Monitoring ions of MP and internal standard (hydroxypioglitazone) were m/z 268.1/115.6 and m/z 373.1/150.2, respectively. The linear range was 3.03–416.35 ng/mL with an average correlation coefficient of 0.9996, and the limit of quantification was 3.03 ng/mL. The intra- and inter-day precision was less than 15%. At low, middle and high concentrations, the recovery, the matrix effect and the accuracy was in the range of 76.06%–95.25%, 93.67%–104.19% and 95.20%–99.96% respectively. The method was applied for the pharmacokinetics study of MP tartrate tablets (50 mg). The AUC0-t, Tmax and Cmax were respectively 919.88 ± 195.67 μg/L·h, 0.96 ± 0.33 h, 349.12 ± 78.04 ng/mL.
Keywords: metoprolol tartrate; LC-MS/MS; pharmacokinetics; protein precipitation method metoprolol tartrate; LC-MS/MS; pharmacokinetics; protein precipitation method
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Li, S.; Wang, X.; Peng, K.; Ma, Z.; Zhang, X.; Fu, S.; Li, X.; Li, L.; Hong, A.; Jiang, J. Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications. Molecules 2012, 17, 2663-2674.

AMA Style

Li S, Wang X, Peng K, Ma Z, Zhang X, Fu S, Li X, Li L, Hong A, Jiang J. Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications. Molecules. 2012; 17(3):2663-2674.

Chicago/Turabian Style

Li, Sha; Wang, Xingli; Peng, Kelong; Ma, Zhiguo; Zhang, Xiaoqi; Fu, Shaolian; Li, Xiaofei; Li, Linlin; Hong, Aihua; Jiang, Jie. 2012. "Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications." Molecules 17, no. 3: 2663-2674.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert