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Toxicological Assessment of β-(1à6)-Glucan (Lasiodiplodan) in Mice during a 28-Day Feeding Study by Gavage
Pharmaceutical Science and Biodiversity Postgraduate Programs, Midwest State University, Campus CEDETEG, Guarapuava, PR, 85040-080, Brazil
Department of Chemistry, Federal Technological University of Parana, Pato Branco, PR, 85503-390, Brazil,
Biorefining Research Institute, Lakehead University, Thunder Bay, ON P7B 5E1, Canada
* Author to whom correspondence should be addressed.
Received: 29 September 2012; in revised form: 19 November 2012 / Accepted: 20 November 2012 / Published: 3 December 2012
Abstract: Studies evaluating the toxicity caused by fungal exopolysaccharides of the β-(1→6)-D-glucan type are rare. In this study, the toxicological effects of sub-chronic treatments with lasiodiplodan (β-(1→6)-D-glucan from Lasiodiplodia theobromae MMPI) were evaluated in mice through the assessment of biochemical, hematological, and histopathological alterations. Thirty-two mice (16 male, 16 female) were used in this study divided in two groups; one group received lasiodiplodan (50 mg/kg body weight) daily for 28 days via gavage, and another (control group) received saline during the same period. Blood samples were collected via cardiac puncture for hematological and biochemical analyses. Liver, heart, kidney, and spleen were collected for histopathological analysis. Statistical analysis was performed through one-way analysis of variance and only p < 0.05 F-values were presented. Significant reduction in blood glucose in the male group (35%; p < 0.01), transaminases activity in both sexes (AST and ALT; ~35%; p < 0.05), and urea (20%; p < 0.01) in the female group was observed with the lasiodiplodan treatment. The results showed that sub-chronic treatments with lasiodiplodan did not generate hematological and histopathological alterations leading to signs of toxicity in healthy mice, independent of gender.
Keywords: Lasiodiplodia theobromae MMPI; fungal β-glucan; toxicity evaluation; Swiss albino mice
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Túrmina, J.A.; Carraro, E.; Alves da Cunha, M.A.; Dekker, R.F.H.; Barbosa, A.M.; dos Santos, F.S.; Silva, L.A.; Malfatti, C.R.M. Toxicological Assessment of β-(1à6)-Glucan (Lasiodiplodan) in Mice during a 28-Day Feeding Study by Gavage. Molecules 2012, 17, 14298-14309.
Túrmina JA, Carraro E, Alves da Cunha MA, Dekker RFH, Barbosa AM, dos Santos FS, Silva LA, Malfatti CRM. Toxicological Assessment of β-(1à6)-Glucan (Lasiodiplodan) in Mice during a 28-Day Feeding Study by Gavage. Molecules. 2012; 17(12):14298-14309.
Túrmina, Janaína A.; Carraro, Emerson; Alves da Cunha, Mário A.; Dekker, Robert F.H.; Barbosa, Aneli M.; dos Santos, Fábio S.; Silva, Luiz A.; Malfatti, Carlos R.M. 2012. "Toxicological Assessment of β-(1à6)-Glucan (Lasiodiplodan) in Mice during a 28-Day Feeding Study by Gavage." Molecules 17, no. 12: 14298-14309.