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Molecules 2012, 17(12), 14219-14229; doi:10.3390/molecules171214219

An Improved Method for Removal of Azo Dye Orange II from Textile Effluent Using Albumin as Sorbent

1 Technical School SENAI-Paulista, Paulista 53417-710, PE, Brazil 2 Nucleus of Research in Environmental Sciences-NPCIAMB, Catholic University of Pernambuco, UNICAP, 50050-590 Recife, PE, Brazil 3 Network of Biotechnology, Catholic University of Pernambuco, Recife 50050-900, PE, Brazil 4 Microbiology Department, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil 5 Chemical Engineering Department, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
* Author to whom correspondence should be addressed.
Received: 10 October 2012 / Revised: 24 November 2012 / Accepted: 27 November 2012 / Published: 30 November 2012
(This article belongs to the Section Metabolites)
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Azo dyes are generally resistant to biodegradation due to their complex structures. Acid orange II is one of the most widely used dyes in the textile industry. The influence of bovine serum albumin (BSA) in different concentrations, pH, and time of contact on Orange II was investigated using kinetics and adsorption-isotherm experiments. The results showed that the maximum colour removed from dye/albumin was 99.50% and that a stable dye-protein complex had been formed at pH 3.5 and in a proportion of 1:3 (v/v), respectively. The synthetic effluent did not show toxicity to the microcrustacean Artemia salina, and showed a CL50 equal to 97 µg/mL to azo dye orange II. Additionally, the methodology was effective in removing the maximum of orange II using BSA by adsorption at pH 3.5 which mainly attracted ions to the azo dye during the adsorption process. This suggests that this form of treatment is economical and easy to use which potentially could lead to bovine serum albumin being used as a sorbent for azo dyes.
Keywords: acid orange II; albumin; decolourization; toxicity; adsorption; isotherm acid orange II; albumin; decolourization; toxicity; adsorption; isotherm
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ohashi, T.; Jara, A.M.T.; Batista, A.C.L.; Franco, L.O.; Lima, M.A.B.; Benachour, M.; da Silva, C.A.A.; Campos-Takaki, G.M. An Improved Method for Removal of Azo Dye Orange II from Textile Effluent Using Albumin as Sorbent. Molecules 2012, 17, 14219-14229.

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