Molecules 2012, 17(12), 13825-13843; doi:10.3390/molecules171213825
Article

Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays

Anna Aviñó 1,2 email, Maria José Gómara 3 email, Morteza Malakoutikhah 3 email, Isabel Haro 3 email and Ramon Eritja 1,2,* email
1 Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Jordi Girona 18, E-08034 Barcelona, Spain 2 Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, E-08028 Barcelona, Spain 3 Unit of Synthesis & Biomedical Applications of Peptides, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Jordi Girona 18, E-08034 Barcelona, Spain
* Author to whom correspondence should be addressed.
Received: 17 October 2012; in revised form: 16 November 2012 / Accepted: 16 November 2012 / Published: 22 November 2012
(This article belongs to the Special Issue Nucleic Acid Analogs)
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Abstract: Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients.
Keywords: oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides

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MDPI and ACS Style

Aviñó, A.; Gómara, M.J.; Malakoutikhah, M.; Haro, I.; Eritja, R. Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays. Molecules 2012, 17, 13825-13843.

AMA Style

Aviñó A, Gómara MJ, Malakoutikhah M, Haro I, Eritja R. Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays. Molecules. 2012; 17(12):13825-13843.

Chicago/Turabian Style

Aviñó, Anna; Gómara, Maria J.; Malakoutikhah, Morteza; Haro, Isabel; Eritja, Ramon. 2012. "Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays." Molecules 17, no. 12: 13825-13843.

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