Next Article in Journal
Influence of Nonpolar Substances on the Extraction Efficiency of Six Alkaloids in Zoagumhwan Investigated by Ultra Performance Liquid Chromatography and Photodiode Array Detection
Next Article in Special Issue
Supramolecular Ring Structures of 7-Methylguanine: A Computational Study of Its Self-assembly and Anion Binding
Previous Article in Journal
Lipases as Tools in the Synthesis of Prodrugs from Racemic 9-(2,3-Dihydroxypropyl)adenine
Previous Article in Special Issue
5'-Chloro-5'-deoxy-(±)-ENBA, a Potent and Selective Adenosine A1 Receptor Agonist, Alleviates Neuropathic Pain in Mice Through Functional Glial and Microglial Changes without Affecting Motor or Cardiovascular Functions
Molecules 2012, 17(12), 13825-13843; doi:10.3390/molecules171213825
Article

Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays

1,2
, 3
, 3
, 3
 and 1,2,*
Received: 17 October 2012; in revised form: 16 November 2012 / Accepted: 16 November 2012 / Published: 22 November 2012
(This article belongs to the Special Issue Nucleic Acid Analogs)
View Full-Text   |   Download PDF [285 KB, updated 18 June 2014; original version uploaded 18 June 2014]   |   Browse Figures
Abstract: Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients.
Keywords: oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides oligonucleotide-peptide conjugates; ELISA; solid-phase synthesis; rheumatoid arthritis; epitopes; anti-citrullinated protein/peptide antibodies; ACPA; fibrin; filaggrin; citrullinated peptides
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Aviñó, A.; Gómara, M.J.; Malakoutikhah, M.; Haro, I.; Eritja, R. Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays. Molecules 2012, 17, 13825-13843.

AMA Style

Aviñó A, Gómara MJ, Malakoutikhah M, Haro I, Eritja R. Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays. Molecules. 2012; 17(12):13825-13843.

Chicago/Turabian Style

Aviñó, Anna; Gómara, Maria J.; Malakoutikhah, Morteza; Haro, Isabel; Eritja, Ramon. 2012. "Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays." Molecules 17, no. 12: 13825-13843.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert