Molecules 2012, 17(10), 11800-11815; doi:10.3390/molecules171011800
Article

Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis

Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
* Author to whom correspondence should be addressed.
Received: 14 August 2012; in revised form: 27 September 2012 / Accepted: 28 September 2012 / Published: 9 October 2012
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Abstract: Streptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. Culturing and environmental factors causing structural differences in mutans were specified. All of the obtained biopolymers (76 samples) were water-insoluble and most of them (72) had a structure with a predominance of α-(1→3)-linked glucose (i.e., the content of α-(1→3)-linkages in the glucan was always higher than 50%, but did not exceed 76%). An exception were four glucans containing more than 50% of α-(1→6)-sequences. In these structurally unique mutans, the ratio of α-(1→3)- to α-(1→6)-bonds ranged from 0.75 to 0.97. Aside from one polymer, all others had a heavily branched structures and differed in the number of α-(1→3), α-(1→6), and α-(1→3,6) linkages and their mutual proportion. The induction of mutanase production in shaken flask cultures of Trichoderma harzianum by the structurally diverse mutans resulted in enzyme activities ranging from 0.144 to 1.051 U/mL. No statistical correlation was found between the total percentage content of α-(1→3)-linkages in the α-glucan and mutanase activity. Thus, despite biosynthetic differences causing structural variation in the mutans, it did not matter which mutan structures were used to induce mutanase production.
Keywords: mutan; glucosyltransferase; cariogenic streptococci; NMR spectroscopy

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MDPI and ACS Style

Wiater, A.; Pleszczyńska, M.; Próchniak, K.; Szczodrak, J. Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis. Molecules 2012, 17, 11800-11815.

AMA Style

Wiater A, Pleszczyńska M, Próchniak K, Szczodrak J. Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis. Molecules. 2012; 17(10):11800-11815.

Chicago/Turabian Style

Wiater, Adrian; Pleszczyńska, Małgorzata; Próchniak, Katarzyna; Szczodrak, Janusz. 2012. "Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis." Molecules 17, no. 10: 11800-11815.

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