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Molecules 2012, 17(1), 303-314; doi:10.3390/molecules17010303
Article

Enantiomeric Separation and Determination of the Enantiomeric Impurity of Armodafinil by Capillary Electrophoresis with Sulfobutyl Ether-β-cyclodextrin as Chiral Selector

1, 2, 1, 1 and 3,4,*
Received: 16 December 2011; in revised form: 27 December 2011 / Accepted: 28 December 2011 / Published: 30 December 2011
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Abstract: A selective capillary electrophoresis method using sulfobutyl ether-β-cyclodextrin as a chiral selector was developed and validated for the determination of the enantiomeric impurity of (R)-modafinil, i.e., armodafinil. Several parameters were optimized for a satisfactory enantioresolution, including the type and concentration of chiral selector and organic modifier, pH of background electrolyte (BGE), capillary temperature. The finally adopted condition was: 20 mmol/L phosphate buffer at pH 7.5, containing 20 mmol/L sulfobutyl ether-β-cyclodextrin and 20% methanol, at temperature of 25 °C. A good resolution of 3.3 for the two enantiomers of modafinil was achieved by applying the optimal conditions. The limit of detection (LOD) and limit of quantification (LOQ) of (S)-modafinil were 1.25 μg/mL and 2.50 μg/mL, respectively. The established method was also proven to display good selectivity, repeatability, linearity and accuracy. Finally, the method was used to investigate the enantiomeric purity of armodafinil in bulk samples.
Keywords: capillary electrophoresis; armodafinil; enantiomeric impurity; sulfobutyl ether-β-cyclodextrin capillary electrophoresis; armodafinil; enantiomeric impurity; sulfobutyl ether-β-cyclodextrin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, W.; Xiang, S.; Zhou, X.; Ji, Y.; Xiang, B. Enantiomeric Separation and Determination of the Enantiomeric Impurity of Armodafinil by Capillary Electrophoresis with Sulfobutyl Ether-β-cyclodextrin as Chiral Selector. Molecules 2012, 17, 303-314.

AMA Style

Wang W, Xiang S, Zhou X, Ji Y, Xiang B. Enantiomeric Separation and Determination of the Enantiomeric Impurity of Armodafinil by Capillary Electrophoresis with Sulfobutyl Ether-β-cyclodextrin as Chiral Selector. Molecules. 2012; 17(1):303-314.

Chicago/Turabian Style

Wang, Wei; Xiang, Suyun; Zhou, Xiaojuan; Ji, Yibing; Xiang, Bingren. 2012. "Enantiomeric Separation and Determination of the Enantiomeric Impurity of Armodafinil by Capillary Electrophoresis with Sulfobutyl Ether-β-cyclodextrin as Chiral Selector." Molecules 17, no. 1: 303-314.


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