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Molecules 2011, 16(9), 7949-7957; doi:10.3390/molecules16097949
Article

Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

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Received: 18 July 2011; in revised form: 24 August 2011 / Accepted: 25 August 2011 / Published: 14 September 2011
(This article belongs to the Section Metabolites)
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Abstract: Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.
Keywords: Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

He, F.; Bai, Y.; Wang, J.; Wei, J.; Yu, C.; Li, S.; Yang, W.; Han, C. Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 7949-7957.

AMA Style

He F, Bai Y, Wang J, Wei J, Yu C, Li S, Yang W, Han C. Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography. Molecules. 2011; 16(9):7949-7957.

Chicago/Turabian Style

He, Fa; Bai, Yuhua; Wang, Jing; Wei, Jing; Yu, ChunYue; Li, Sen; Yang, Weili; Han, Chenghua. 2011. "Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography." Molecules 16, no. 9: 7949-7957.


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