Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

doi:10.3390/molecules16097949

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Molecules 2011, 16(9), 7949-7957; doi:10.3390/molecules16097949

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Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

Fa He¹, Yuhua Bai^1,* , Jing Wang¹, Jing Wei², ChunYue Yu¹, Sen Li¹, Weili Yang¹ and Chenghua Han¹

¹ College of Pharmacy, Harbin University, Daqing 163319, China ² Petrochemical Research Institute Daqing Petrochemical Research Center, Daqing 163714, China

* Author to whom correspondence should be addressed.

Received: 18 July 2011; in revised form: 24 August 2011 / Accepted: 25 August 2011 / Published: 14 September 2011

(This article belongs to the Section Natural Products)

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Abstract: Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, ¹H-NMR and ¹³C-NMR.

Keywords: Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin

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Cite This Article

MDPI and ACS Style

He, F.; Bai, Y.; Wang, J.; Wei, J.; Yu, C.; Li, S.; Yang, W.; Han, C. Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 7949-7957.

AMA Style

He F, Bai Y, Wang J, Wei J, Yu C, Li S, Yang W, Han C. Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography. Molecules. 2011; 16(9):7949-7957.

Chicago/Turabian Style

He, Fa; Bai, Yuhua; Wang, Jing; Wei, Jing; Yu, ChunYue; Li, Sen; Yang, Weili; Han, Chenghua. 2011. "Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography." Molecules 16, no. 9: 7949-7957.

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