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Molecules 2011, 16(9), 7949-7957; doi:10.3390/molecules16097949

Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

1
College of Pharmacy, Harbin University, Daqing 163319, China
2
Petrochemical Research Institute Daqing Petrochemical Research Center, Daqing 163714, China
*
Author to whom correspondence should be addressed.
Received: 18 July 2011 / Revised: 24 August 2011 / Accepted: 25 August 2011 / Published: 14 September 2011
(This article belongs to the Section Natural Products)
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Abstract

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.
Keywords: Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

He, F.; Bai, Y.; Wang, J.; Wei, J.; Yu, C.; Li, S.; Yang, W.; Han, C. Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 7949-7957.

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